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Animals : an open access journal from MDPI2021; 11(12); 3379; doi: 10.3390/ani11123379

Ion Channel and Ubiquitin Differential Expression during Erythromycin-Induced Anhidrosis in Foals.

Abstract: Macrolide drugs are the treatment of choice for infections, despite severe side-effects temporary anhidrosis as a. To better understand the molecular biology leading to macrolide induced anhidrosis, we performed skin biopsies and Quantitative Intradermal Terbutaline Sweat Tests (QITSTs) in six healthy pony-cross foals for three different timepoints during erythromycin administration-pre-treatment (baseline), during anhidrosis and post-recovery. RNA sequencing of biopsies followed by differential gene expression analysis compared both pre and post normal sweating timepoints to the erythromycin induced anhidrosis episode. After Bonferroni correction for multiple testing, 132 gene transcripts were significantly differentially expressed during the anhidrotic timepoint. Gene ontology analysis of the full differentially expressed gene set identified over-represented biological functions for ubiquitination and ion-channel function, both biologically relevant to sweat production. These same mechanisms were previously implicated in heritable equine idiopathic anhidrosis and sweat gland function and their involvement in macrolide-induced temporary anhidrosis warrants further investigation.
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Publication Date: 2021-11-25 PubMed ID: 34944156PubMed Central: PMC8697959DOI: 10.3390/ani11123379Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study investigates how the drug erythromycin, a widely-used macrolide antibiotic, affects the molecular biology behind sweat production in young horses. Researchers found that during periods of erythromycin administration, gene expressions related to ubiquitination and ion-channel functions, both crucial to sweat production, changed significantly.

Research Methodology

  • The researchers hired six healthy pony-cross foals as test subjects for this experiment.
  • They performed skin biopsies and Quantitative Intradermal Terbutaline Sweat Tests (QITSTs) on these foals.
  • The testing was conducted at three different stages during the erythromycin treatment: before the treatment (baseline), during the anhidrosis (inability to sweat) period, and after recovery.

Analysis and Findings

  • The team executed RNA sequencing of the biopsies and subsequntly analyzed for differential gene expression. They compared the gene expressions at normal sweat production timepoints (both before and after erythromycin treatment) to those during the anhidrotic episode induced by erythromycin.
  • After implementing a Bonferroni correction to adjust for multiple testing, the team identified 132 gene transcripts that were significantly differentially expressed during the anhidrotic period.
  • A gene ontology analysis was carried out on the all differentially expressed genes. The researchers discovered that the functions most represented in the gene set were ubiquitination and ion-channel function.

Significance of the Findings

  • Ubiquitination and ion-channel function are both known to be biologically relevant to sweat production. Macrolide-induced anhidrosis is a serious side-effect that can severely impact the health of foals.
  • These mechanisms have been previously associated with heritable equine idiopathic anhidrosis and sweat gland function. Their involvement in temporary anhidrosis caused by macrolides warrants further investigation to better understand these side-effects and develop possible treatments.

Cite This Article

APA
Patterson Rosa L, Mallicote MF, MacKay RJ, Brooks SA. (2021). Ion Channel and Ubiquitin Differential Expression during Erythromycin-Induced Anhidrosis in Foals. Animals (Basel), 11(12), 3379. https://doi.org/10.3390/ani11123379

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 11
Issue: 12
PII: 3379

Researcher Affiliations

Patterson Rosa, Laura
  • Department of Animal Sciences, UF Genetics Institute, University of Florida, Gainesville, FL 32611, USA.
  • Etalon Diagnostics, Menlo Park, CA 94025, USA.
Mallicote, Martha F
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.
MacKay, Robert J
  • Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA.
Brooks, Samantha A
  • Department of Animal Sciences, UF Genetics Institute, University of Florida, Gainesville, FL 32611, USA.

Grant Funding

  • 2015 grant cycle / Grayson-Jockey Club Research Foundation

Conflict of Interest Statement

Samantha A. Brooks has been involved as a consultant at Etalon Diagnostics, a genetic testing company. The authors declare no conflict of interest.

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Citations

This article has been cited 1 times.
  1. Rakowska A, Marciniak-Karcz A, Bereznowski A, Cywińska A, Żychska M, Witkowski L. Less Typical Courses of Rhodococcus equi Infections in Foals. Vet Sci 2022 Oct 31;9(11).
    doi: 10.3390/vetsci9110605pubmed: 36356082google scholar: lookup
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