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Home / Equine Research Bank / Open Vet J / Isolated limb perfusion electrochemotherapy for the treatmen...
Open veterinary journal2017; 7(2); 192-196; doi: 10.4314/ovj.v7i2.18

Isolated limb perfusion electrochemotherapy for the treatment of an advanced squamous cell carcinoma of the hoof in a mare.

Abstract: A twenty-year-old female saddle horse was referred for evaluation of a seven month, non-healing erosive lesion of the right hind hoof with proliferation and bleeding of the underlying soft tissues. This lesion had been twice surgically treated as a canker but rapidly recurred. Histological examination of the second excision revealed a well-differentiated squamous cell carcinoma. At presentation, the horse was mildly depressed, lame and partially non-weight-bearing on the right hind leg, which exhibited a 10 x 10 cm erosive and proliferative lesion remodeling the hoof. After completing staging procedures, the lesion was approached with surgery and intraoperative electrochemotherapy (ECT) administration of bleomycin in isolated limb perfusion. A second session of surgery and ECT was performed one month later, followed by three additional monthly sessions of ECT. During periodic recheck, the mare showed continuous improvement. One year after presentation, the mare was in complete remission and her gait markedly improved. ECT was well-tolerated and resulted in improved local control of a tumor in a challenging anatomical district.
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Publication Date: 2017-06-28 PubMed ID: 28717603PubMed Central: PMC5498771DOI: 10.4314/ovj.v7i2.18Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article discusses a successful case of treating advanced squamous cell carcinoma in the hoof of a mare using a combination of surgery and electrochemotherapy (ECT) with isolated limb perfusion.

Introduction

  • The article begins with background information about the case: a twenty-year-old saddle horse presenting an advanced squamous cell carcinoma in the right hind hoof. This horse had previously undergone two unsuccessful surgeries for what had been diagnosed as a canker.
  • Upon reevaluation, the cancerous tumor was identified and the horse was reported to be suffering from depression and lameness, bearing little weight on the afflicted hind leg.

Treatment Approach

  • The research team opted for a combined treatment approach involving surgery and intraoperative electrochemotherapy (ECT), a method that uses electric pulses to increase the effect of chemotherapy.
  • The chemotherapy drug used in this treatment process was bleomycin, which was administered using isolated limb perfusion — a process that targets the drug delivery to a specific area, in this case, the affected leg.

Procedure and Follow-up

  • The treatment procedure included two sessions of surgery and ECT with an interval of one month in between, followed by three additional monthly ECT sessions.
  • During the follow-up period, the mare consistently showed improvement, and one year after the initial presentation, the horse was in complete remission and her gait had significantly improved, pointing towards the successful control of the tumor.

Conclusion

  • The findings from this case study demonstrate that ECT in combination with surgery can successfully treat advanced squamous cell carcinoma in horses. It presents an effective solution that is well-tolerated by the animal, and provides increased local control in areas challenging to treat.
  • This case study could potentially guide future research into the treatment of cancer in horses, highlighting the potential benefits of electrochemotherapy.

Cite This Article

APA
Spugnini EP, Bolaffio C, Scacco L, Baldi A. (2017). Isolated limb perfusion electrochemotherapy for the treatment of an advanced squamous cell carcinoma of the hoof in a mare. Open Vet J, 7(2), 192-196. https://doi.org/10.4314/ovj.v7i2.18

Publication

Open veterinary journal
ISSN: 2226-4485
NlmUniqueID: 101653182
Country: Libya
Language: English
Volume: 7
Issue: 2
Pages: 192-196

Researcher Affiliations

Spugnini, Enrico Pierluigi
  • Biopulse S.r.l., Naples, Italy.
  • Equivet Roma Hospital, Rome, Italy.
Bolaffio, Carlo
  • Equivet Roma Hospital, Rome, Italy.
Scacco, Licia
  • Equivet Roma Hospital, Rome, Italy.
Baldi, Alfonso
  • Biopulse S.r.l., Naples, Italy.
  • Department of Environmental, Biological and Pharmaceutical Sciences and Technologies Campania University "Luigi Vanvitelli", Caserta, Italy.

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This article includes 22 references
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Citations

This article has been cited 3 times.
  1. Stefanik E, Górski K, Turek B, Drewnowska-Szczepakowska O, Kliczkowska-Klarowicz K, Stefanik A. From Keratoma to Anaplastic Malignant Melanoma in a Horse's Hoof. Animals (Basel) 2022 Nov 9;12(22).
    doi: 10.3390/ani12223090pubmed: 36428318google scholar: lookup
  2. Spugnini EP, Scacco L, Bolaffio C, Baldi A. Electrochemotherapy for the treatment of cutaneous solid tumors in equids: A retrospective study. Open Vet J 2021 Jul-Sep;11(3):385-389.
    doi: 10.5455/OVJ.2021.v11.i3.8pubmed: 34722200google scholar: lookup
  3. Condello M, D'Avack G, Vona R, Spugnini EP, Scacco L, Meschini S. Electrochemotherapy with Mitomycin C Potentiates Apoptosis Death by Inhibiting Autophagy in Squamous Carcinoma Cells. Cancers (Basel) 2021 Jul 31;13(15).
    doi: 10.3390/cancers13153867pubmed: 34359775google scholar: lookup
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