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Epidemiology and infection2013; 142(4); 759-766; doi: 10.1017/S095026881300143X

Isolation and genetic characterization of naturally NS-truncated H3N8 equine influenza virus in South Korea.

Abstract: Equine influenza virus (EIV) causes a highly contagious respiratory disease in equids, with confirmed outbreaks in Europe, America, North Africa, and Asia. Although China, Mongolia, and Japan have reported equine influenza outbreaks, Korea has not. Since 2011, we have conducted a routine surveillance programme to detect EIV at domestic stud farms, and isolated H3N8 EIV from horses showing respiratory disease symptoms. Here, we characterized the genetic and biological properties of this novel Korean H3N8 EIV isolate. This H3N8 EIV isolate belongs to the Florida sublineage clade 1 of the American H3N8 EIV lineage, and surprisingly, possessed a non-structural protein (NS) gene segment, where 23 bases of the NS1-encoding region were naturally truncated. Our preliminary biological data indicated that this truncation did not affect virus replication; its effect on biological and immunological properties of the virus will require further study.
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Publication Date: 2013-06-26 PubMed ID: 23800580PubMed Central: PMC9151127DOI: 10.1017/S095026881300143XGoogle Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The researchers isolated and genetically characterized H3N8 equine influenza virus from a horse in South Korea, a previously unaffected area. The novel virus belongs to the Florida sublineage clade 1 of the American H3N8 lineage and features a naturally truncated non-structural protein gene segment. The impact of this truncation on the virus’s properties needs additional investigation.

Isolating and Identification of H3N8 Influenza in South Korea

  • The researchers conducted a routine surveillance program from 2011 onwards, aimed at detecting the presence of equine influenza virus (EIV) at local stud farms in South Korea.
  • During these inspections, they isolated the H3N8 EIV subtype from horses exhibiting symptoms of a respiratory disease.
  • This finding marked the first confirmation of an EIV outbreak in South Korea, an area previously thought to be unaffected by equine influenza.

Genetic Characterization of South Korean H3N8 EIV

  • The newly isolated South Korean H3N8 EIV was genetically examined to understand its evolutionary lineage and specific properties.
  • The analysis revealed that the new virus strain belongs to the Florida sublineage clade 1, which is a part of the American H3N8 EIV lineage.
  • This characterizes the virus as genetically similar to those found in recent outbreaks in the United States.

Natural Truncation in the Non-Structural Protein Gene Segment

  • Interestingly, the South Korean H3N8 EIV featured a naturally occurring truncation in its non-structural protein (NS) gene segment.
  • Specifically, 23 bases of the NS1-encoding region within the NS gene were found to be missing or truncated, which is unusual in influenza viruses.
  • This truncation was found not to affect the virus’s ability to replicate in preliminary analyses, but the ramifications of this mutation on the overall biological and immunological properties of the virus are still unclear and need to be explored further.

Cite This Article

APA
Na W, Kang B, Kim HI, Hong M, Park SJ, Jeoung HY, An DJ, Moon H, Kim JK, Song D. (2013). Isolation and genetic characterization of naturally NS-truncated H3N8 equine influenza virus in South Korea. Epidemiol Infect, 142(4), 759-766. https://doi.org/10.1017/S095026881300143X

Publication

Epidemiology and infection
ISSN: 1469-4409
NlmUniqueID: 8703737
Country: England
Language: English
Volume: 142
Issue: 4
Pages: 759-766

Researcher Affiliations

Na, W
  • Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
Kang, B
  • Research Unit, Green Cross Veterinary Products, Yong-in, South Korea.
Kim, H-I
  • Optifarm Solution, Chungnam, South Korea.
Hong, M
  • Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
Park, S-J
  • Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.
Jeoung, H-Y
  • Animal, Plant and Fisheries Quarantine and Inspection Agency, Gyeonggi, South Korea.
An, D-J
  • Animal, Plant and Fisheries Quarantine and Inspection Agency, Gyeonggi, South Korea.
Moon, H
  • Research Unit, Green Cross Veterinary Products, Yong-in, South Korea.
Kim, J-K
  • Department of Pharmacy, College of Pharmacy, Korea University, Sejong, South Korea.
Song, D
  • Viral Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, South Korea.

MeSH Terms

  • Animals
  • Base Sequence
  • Dogs
  • Horses
  • Influenza A Virus, H3N8 Subtype / classification
  • Influenza A Virus, H3N8 Subtype / genetics
  • Influenza A Virus, H3N8 Subtype / isolation & purification
  • Madin Darby Canine Kidney Cells
  • Molecular Sequence Data
  • Nasal Cavity / virology
  • Orthomyxoviridae Infections / veterinary
  • Orthomyxoviridae Infections / virology
  • Phylogeny
  • Republic of Korea
  • Viral Nonstructural Proteins / genetics
  • Virus Cultivation
  • Virus Replication

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This article has been cited 7 times.
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