Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.
Abstract: This study was conducted to isolate and identify the cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigate their effect on the nutritional value of wheat straw (WS). Results: Fresh faeces were collected from four Arabian horses. The cellulose-hydrolytic bacteria were isolated by using a medium amended with carboxymethyl cellulose (CMC). The activity of CMC was determined by measuring the release of reducing sugars from CMC. Among the isolates, four isolates (L11, L12, L2 and Z2) showed maximum hydrolysis capacity. 16S rRNA sequence analysis showed that these isolates possessed 99, 99, 99 and 98% similarity with Paenibacillus polymyxa, Paenibacillus polymyxa, Enterobacter cloacae and Escherichia coli respectively. Wheat straw was incubated with the isolated bacteria in liquid medium. Disappeared dry matter (DM) and crude protein (CP), neutral detergent fibre (NDF), indigestible NDF (iNDF) and acid detergent fibre (ADF) contents of WS were affected by microbial treatments (P < 0·05). The highest disappearance of DM and CP contents and the lowest NDF, iNDF and ADF contents of WS were observed by treating with P. polymyxa L11. The values of NDF, iNDF and ADF were the highest and CP was the lowest in control. Gas production parameters and digestibility of processed samples were determined. Bacterial treatments enhanced (P < 0·05) DM, OM, CP, NDF and ADF digestibility of WS. The highest and lowest (P < 0·05) DM, OM, CP, NDF and ADF digestibilities were observed for WS treated with isolates L12 and control respectively. Potential of gas production (b), truly degraded organic matter (TDOM) and microbial biomass of bacteria treatments were significantly higher (P < 0·05) compared to control. Conclusions: Generally, the results of this study showed that the isolated bacteria from horse faeces are capable of changing the chemical composition, increasing digestibility as well as enhancing nutrition value of WS. Conclusions: Wheat straw is a major agro-residue fed to ruminants. However, high fibre contents reduce digestibility and limit animal productivity. It seems that enhancement of ruminal degradation of these by-products is necessary. The results of this study revealed that cellulolytic bacteria isolated from gastrointestinal tract of horse can be used for biological treatment of WS.
© 2019 The Society for Applied Microbiology.
Publication Date: 2019-06-07 PubMed ID: 30873704DOI: 10.1111/jam.14251Google Scholar: Lookup
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- Journal Article
Summary
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The research explores the isolation and identification of bacteria that can degrade cellulose, extracted from Arabian horse guts. The impact of these bacteria on the nutritional quality of wheat straw, a common animal feed, was also investigated.
Methodology
- Firstly, the sample for the study, fresh fecal matter, was secured from four Arabian horses.
- Then, the cellulose-hydrolytic bacteria were isolated. This was achieved by using a medium mixed with carboxymethyl cellulose (CMC).
- The CMC activity was assessed by quantifying the release of reducing sugars from the CMC reacting with the isolated bacteria.
Results of Bacteria Isolation
- A few of the isolated bacteria showed a high cellulose hydrolysis capacity. These isolates, labeled as L11, L12, L2, and Z2, were identified by 16S rRNA sequence analysis.
- The results showed these isolates were similar, by percentages ranging from 98% to 99%, to known bacteria including Paenibacillus polymyxa and Enterobacter cloacae.
Effect on Wheat Straw
- The wheat straw was subsequently exposed to these isolated bacteria. The aim was to observe the impact these bacteria had on the feed’s nutritional value and overall digestibility.
- Measurements on the amount of disappeared dry matter (DM) and crude protein (CP), along with the neutral detergent fiber (NDF), indigestible NDF (iNDF), and acid detergent fiber (ADF) content of the wheat straw were taken.
- The most significant changes were observed upon treating the straw with isolate bacteria P. polymyxa L11.
- Gas production parameters were also researched, providing insight into the digestive process and extent of nutrient release.
Conclusions
- Overall, the research concludes that the bacteria isolated from equine feces have the potential to alter the nutritive properties of common livestock feeds, specifically wheat straw.
- They do this by changing the chemical composition, improving digestibility, and increasing the feed’s nutritional value. This indicates the potential these bacteria have in enhancing the use of high-fiber agro-residues like wheat straw in livestock feeding, thus improving animal productivity.
Cite This Article
APA
Shakarami MH, Mohammadabadi T, Motamedi H, Sari M, Teimouri Yansari A.
(2019).
Isolation and identification of cellulolytic bacteria from gastrointestinal tract of Arabian horse and investigation of their effect on the nutritional value of wheat straw.
J Appl Microbiol, 127(2), 344-353.
https://doi.org/10.1111/jam.14251 Publication
Researcher Affiliations
- Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran.
- Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran.
- Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Khuzestan, Iran.
- Biotechnology and Biological Science Research Center, Shahid Chamran University of Ahvaz, Khuzestan, Iran.
- Department of Animal Science, Faculty of Animal Science and Food Technology, Agricultural Sciences and Natural Resources University of Khuzestan, Mollasani, Ahvaz, Iran.
- Department of Animal Science, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
MeSH Terms
- Animal Feed
- Animals
- Bacteria / genetics
- Bacteria / isolation & purification
- Bacteria / metabolism
- Carbohydrates
- Cellulose / metabolism
- Feces / microbiology
- Gastrointestinal Tract / microbiology
- Horses / microbiology
- Nutritive Value
- RNA, Ribosomal, 16S / genetics
- Triticum
Grant Funding
- Agricultural Sciences and Natural Resources University of Khuzestan
Citations
This article has been cited 2 times.- Chen H, Hao D, Chen C, Sun Y, Yu X. Effects of midgut bacteria in Hyphantria cunea (Lepidoptera: Erebidae) on nuclear polyhedrosis virus and Bacillus thuringiensis (Bacillales: Bacillaceae).. J Insect Sci 2023 Mar 1;23(2).
- Wunderlich G, Bull M, Ross T, Rose M, Chapman B. Understanding the microbial fibre degrading communities & processes in the equine gut.. Anim Microbiome 2023 Jan 12;5(1):3.
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