Isolation from the horse genome of a new DNA transposon belonging to the Tigger family.
Abstract: Tigger elements are human DNA transposons homologous to the pogo element of Drosophila melanogaster. They contain an open reading frame for a transposase very similar to the major mammalian centromere protein CENP-B. We found in the horse genome a DNA element ( Ecatig3) sharing 88% homology with human Tigger3. The presence of Tigger elements in the horse genome confirms previous data that date these elements before the divergence between Perissodactyla and Primates (80-90 Myr ago). Copy number evaluation indicates that the horse element is much more abundant than its human counterpart. Southern blot analysis demonstrates that Ecatig3 elements are extremely homogeneous; this may indicate that the evolution of this DNA transposon has been driven by some kind of selection and has not been neutral.
Publication Date: 2004-06-01 PubMed ID: 15170229DOI: 10.1007/s00335-004-3040-6Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research paper unveils a new type of DNA transposon, related to the Tigger family, isolated from the horse genome. The finding of this DNA element, termed as Ecatig3, which shares significant similarity with its human counterpart, offers insights into genomic evolution, suggesting a presence of these elements dating back to a divergence period between Perissodactyla and Primates.
Discovery of New DNA Transposon
- The research focuses on the identification and characterization of a new DNA transposon, a type of mobile genetic element that can change its position within a genome.
- The newly discovered DNA transposon, known as Ecatig3, belongs to the Tigger family, the elements of which are found in human DNA and are known to be homologous to the pogo element of Drosophila melanogaster (common fruit fly).
- The new DNA transposon was isolated from the genome of a horse, adding a new species to the known hosts of Tigger elements.
Homology and Evolutionary Insights
- Ecatig3 shares approximately 88% homology with the human Tigger3, pointing towards a high level of genetic similarity between the two.
- The presence of Tigger elements in the horse genome provides supporting evidence to previous findings that these elements existed prior to the evolutionary split between Perissodactyla (odd-toed ungulates like the horse) and Primates, which is believed to have happened around 80 – 90 million years ago.
Abundance and Homogeneity
- The paper reveals that the number of Ecatig3 elements in the horse genome is much higher than its equivalent in the human genome.
- Using Southern blot analysis, a commonly employed technique to detect specific DNA sequences in DNA samples, the researchers discovered that Ecatig3 elements show much uniformity, indicating a high degree of sequence conservation.
- This uniformity in the sequences of Ecatig3 elements suggests that their evolution might not have been neutral but possibly influenced by a certain selective pressure leading to their conservation.
Cite This Article
APA
Paulis M, Moralli D, Bensi M, De Carli L, Raimondi E.
(2004).
Isolation from the horse genome of a new DNA transposon belonging to the Tigger family.
Mamm Genome, 15(5), 399-403.
https://doi.org/10.1007/s00335-004-3040-6 Publication
Researcher Affiliations
- Department of Genetics and Microbiology A. Buzzati Traverso, University of Pavia, Via Ferrata 1, 27100, Pavia, Italy.
MeSH Terms
- Animals
- Base Sequence
- Blotting, Southern
- DNA / genetics
- DNA Transposable Elements / genetics
- Evolution, Molecular
- Fibroblasts / metabolism
- Gene Dosage
- Genome
- Horses / genetics
- Molecular Sequence Data
- Selection, Genetic
- Sequence Homology, Nucleic Acid
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This article includes 13 references
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Citations
This article has been cited 2 times.- Casadei R, Pelleri MC, Vitale L, Facchin F, Canaider S, Strippoli P, Vian M, Piovesan A, Bianconi E, Mariani E, Piva F, Frabetti F. Characterization of human gene locus CYYR1: a complex multi-transcript system. Mol Biol Rep 2014 Sep;41(9):6025-38.
- Hellen EH, Brookfield JF. Investigation of the origin and spread of a Mammalian transposable element based on current sequence diversity. J Mol Evol 2011 Dec;73(5-6):287-96.
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