English
French
Isolation of a compound from Eupatorium adenophorum (Spreng.) [Ageratina adenophora (Spreng.)] causing hepatotoxicity in mice.
Abstract: Regular ingestion of Eupatorium adenophorum [Ageratina adenophora (Spreng.)] or Crofton weed causes chronic pulmonary disease in horses mainly in Australia, New Zealand, and the Himalayas. The disease is characterized by pulmonary interstitial fibrosis, emphysema, alveolar epithelisation and reduced tolerance to exercise. Horses apparently are the only animals affected and there are numerous reports of farms losing all their horses. The disorder was produced experimentally in horse feeding trials, and it was shown that characteristic lesions occurred in the lungs. In studies with laboratory animals, mice were shown to be suitable test animals, but in this species lesions occur in the liver rather than the lungs. The hepatic injury in these animals is characterized by multiple areas of focal necrosis of the parenchyma associated with degeneration and loss of the epithelium lining the small bile ducts. The active principle 9-oxo-10,11 dehydroagerophorone responsible for these lesions in mice has been isolated from E. adenophorum. Although the compound has been shown to exhibit toxicity to larvae of invertebrate species, no mammalian toxicity studies have been previously reported involving the isolated toxin. The mechanism of the toxic effect of the compound as well as its possible relevance to the respiratory disease in the horse remain to be investigated.
Publication Date: 1995-01-01 PubMed ID: 8581319DOI: 10.1002/nt.2620030505Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research focuses on isolating a compound from the Eupatorium adenophorum plant, known for causing chronic lung disease in horses and liver damage in mice. The identified compound, 9-oxo-10,11 dehydroagerophorone, has not been previously studied for its impact on mammalian toxicity.
Understanding the Problem
- The study underscores a chronic pulmonary disease in horses, primarily reported in Australia, New Zealand, and the Himalayas.
- The disease is marked by pulmonary interstitial fibrosis, emphysema, alveolar epithelisation, and lower exercise tolerance.
- It mentions an experimentally reproduced disorder in horses via feeding trials where lung lesions were observed.
- The disorder seems exclusive to horses, with various incidents of farms losing all their equines.
Mice as Test Subjects
- Conversely, with mice used as test animals in laboratory investigations, the lesions appeared in the liver and not the lungs.
- These liver injuries were characterized by multiple areas of parenchyma focal necrosis coupled with degeneration and loss of the epithelium lining of the small bile ducts.
Isolation of Toxin Compound
- The research successfully isolated the active principle 9-oxo-10,11 dehydroagerophorone from E. adenophorum, which is responsible for causing the liver lesions in mice.
- This particular compound has prior records of exhibiting toxicity to the larvae of certain invertebrate species. However, no reported studies have analyzed its associated mammalian toxicity.
Further Research
- The study concludes by affirming the need for further investigation into the toxic mechanism of the compound.
- The authors point out the potential relevance to the respiratory disease in horses, which remains an open area for study.
Cite This Article
APA
Oelrichs PB, Calanasan CA, MacLeod JK, Seawright AA, Ng JC.
(1995).
Isolation of a compound from Eupatorium adenophorum (Spreng.) [Ageratina adenophora (Spreng.)] causing hepatotoxicity in mice.
Nat Toxins, 3(5), 350-354.
https://doi.org/10.1002/nt.2620030505 Publication
Researcher Affiliations
- National Research Centre for Environmental Toxicology, Brisbane, Australia.
MeSH Terms
- Administration, Oral
- Animals
- Bile Ducts / drug effects
- Bile Ducts / pathology
- Liver / drug effects
- Liver / pathology
- Magnetic Resonance Spectroscopy
- Male
- Mass Spectrometry
- Mice
- Necrosis / chemically induced
- Plant Extracts / chemistry
- Plant Extracts / isolation & purification
- Plant Extracts / toxicity
- Plant Proteins / chemistry
- Plant Proteins / isolation & purification
- Plant Proteins / toxicity
- Sesquiterpenes / administration & dosage
- Sesquiterpenes / chemistry
- Sesquiterpenes / isolation & purification
- Sesquiterpenes / metabolism
- Sesquiterpenes / toxicity
Citations
This article has been cited 17 times.- Shapter FM, Granados-Soler JL, Stewart AJ, Bertin FR, Allavena R. Equine Crofton Weed (Ageratina spp.) Pneumotoxicity: What Do We Know and What Do We Need to Know?. Animals (Basel) 2023 Jun 23;13(13).
- Sun W, Liu SS, Zhao CC. Biological properties of active compounds from Ageratina adenophora.. SAGE Open Med 2023;11:20503121231167964.
- Lan M, Zeng S, Hussain M, Tang P, Ma S, Yi J, Li L, Wang J, Guo J, Wu G, Gao X. Bio-accumulation effects of heavy metals Pb, Zn and Cd on Procecidochares utilis parasitism to Eupatorium adenophorum at Suzu metal mines, Yunnan.. Heliyon 2022 Sep;8(9):e10381.
- Jamloki A, Trivedi VL, Nautiyal MC, Semwal P, Cruz-Martins N. Poisonous Plants of the Indian Himalaya: An Overview.. Metabolites 2022 Jun 13;12(6).
- Ren Z, Okyere SK, Wen J, Xie L, Cui Y, Wang S, Wang J, Cao S, Shen L, Ma X, Yu S, Deng J, Hu Y. An Overview: The Toxicity of Ageratina adenophora on Animals and Its Possible Interventions.. Int J Mol Sci 2021 Oct 27;22(21).
- Cui Y, Okyere SK, Gao P, Wen J, Cao S, Wang Y, Deng J, Hu Y. Ageratina adenophora Disrupts the Intestinal Structure and Immune Barrier Integrity in Rats.. Toxins (Basel) 2021 Sep 15;13(9).
- Ren Z, Gao P, Okyere SK, Cui Y, Wen J, Jing B, Deng J, Hu Y. Ageratina adenophora Inhibits Spleen Immune Function in Rats via the Loss of the FRC Network and Th1-Th2 Cell Ratio Elevation.. Toxins (Basel) 2021 Apr 26;13(5).
- Sun W, Zeng C, Yue D, Liu S, Ren Z, Zuo Z, Deng J, Peng G, Hu Y. Ageratina adenophora causes spleen toxicity by inducing oxidative stress and pyroptosis in mice.. R Soc Open Sci 2019 Jul;6(7):190127.
- Li L, Lan M, Lu W, Li Z, Xia T, Zhu J, Ye M, Gao X, Wu G. De novo transcriptomic analysis of the alimentary tract of the tephritid gall fly, Procecidochares utilis.. PLoS One 2018;13(8):e0201679.
- Mo Q, Hu L, Weng J, Zhang Y, Zhou Y, Xu R, Zuo Z, Deng J, Ren Z, Zhong Z, Peng G, Nong X, Wei Y, Hu Y. Euptox A Induces G1 Arrest and Autophagy via p38 MAPK- and PI3K/Akt/mTOR-Mediated Pathways in Mouse Splenocytes.. J Histochem Cytochem 2017 Sep;65(9):543-558.
- Liu B, Cao L, Zhang L, Yuan X, Zhao B. Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum.. Molecules 2016 Dec 31;22(1).
- He Y, Mo Q, Luo B, Qiao Y, Xu R, Zuo Z, Deng J, Nong X, Peng G, He W, Wei Y, Hu Y. Induction of apoptosis and autophagy via mitochondria- and PI3K/Akt/mTOR-mediated pathways by E. adenophorum in hepatocytes of saanen goat.. Oncotarget 2016 Aug 23;7(34):54537-54548.
- He Y, Mo Q, Hu Y, Chen W, Luo B, Wu L, Qiao Y, Xu R, Zhou Y, Zuo Z, Deng J, He W, Wei Y. E. adenophorum induces Cell Cycle Arrest and Apoptosis of Splenocytes through the Mitochondrial Pathway and Caspase Activation in Saanen Goats.. Sci Rep 2015 Nov 3;5:15967.
- He Y, Chen W, Hu Y, Luo B, Wu L, Qiao Y, Mo Q, Xu R, Zhou Y, Ren Z, Zuo Z, Deng J, Peng G, He W, Wei Y. E. adenophorum Induces Cell Cycle and Apoptosis of Renal Cells through Mitochondrial Pathway and Caspase Activation in Saanen Goat.. PLoS One 2015;10(9):e0138504.
- Hu Y, Liao F, Hu Y, Luo B, He Y, Mo Q, Zuo Z, Ren Z, Deng J, Wei Y. Clinical efficacy of 9-oxo-10, 11-dehydroageraphorone extracted from Eupatorium adenophorum against Psoroptes cuniculi in rabbits.. BMC Vet Res 2014 Dec 20;10:970.
- Ouyang CB, Liu XM, Liu Q, Bai J, Li HY, Li Y, Wang QX, Yan DD, Mao LG, Cao A, Guo MX. Toxicity Assessment of Cadinene Sesquiterpenes from Eupatorium adenophorum in Mice.. Nat Prod Bioprospect 2014 Dec 12;5(1):29-36.
- Kaushal V, Dawra RK, Sharma OP, Kurade NP. Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum.. Vet Res Commun 2001 Oct;25(7):601-8.
Secure Payments
Satisfaction Guarantee
We offer a 60-day guarantee to ensure you are completely satisfied.
Fast & Free Shipping
We provide fast and free shipping on all orders over $40.