Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding.
Abstract: Horses are commonly infected by herpesviruses, but isolation of equine herpesvirus-5 (EHV-5) has only infrequently been reported. We describe the isolation and characterization of a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California. The virus was initially identified by EHV-5 specific polymerase chain reaction (PCR), and it caused lytic infection of cultured rabbit kidney cells only after repeated serial passage. Virions with characteristic herpesvirus morphology were readily demonstrated in cell culture lysate by transmission electron microscopy. A portion of the glycoprotein B gene of this strain of EHV-5 had 99% identity to the published EHV-5 sequence, and it was clearly distinguishable from other EHV (1-4) by virus-specific PCR assays. Prevalence of EHV-5 infection in a group of young racehorses was estimated at 64% using the EHV-5 specific PCR on nasopharyngeal secretions.
Publication Date: 2006-10-14 PubMed ID: 17037617DOI: 10.1177/104063870601800509Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Case Reports
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This study documents the isolation and analysis of the equine herpesvirus-5 (EHV-5) from the blood cells of a horse. The research further provides insights into the disease manifestation of EHV-5 and its prevalence in a young racehorse population.
Isolation of Equine Herpesvirus-5
- The researchers of this study isolated a strain of EHV-5 from the blood mononuclear cells of a healthy adult horse in California.
- The identification of the virus was achieved through a specific polymerase chain reaction (PCR) test designed for EHV-5.
- It was noticed that the virus only caused lytic infection (cell death) of rabbit kidney cells that they were testing on after several passages, suggesting that the virus may not be highly virulent or may require certain conditions to become infective.
Characterization of the virus
- The isolated virus was examined under a transmission electron microscope where virions (virus particles) with the characteristic shape of herpesviruses were identified in the cell culture.
- Glycoprotein B gene of this EHV-5 strain showed 99% similarity to the already documented EHV-5 sequence. This similarity affirms the identity of the virus as EHV-5.
- Furthermore, unique distinguishing features of this strain from other equine herpesviruses (EHV-1 to EHV-4) were identified through virus-specific PCR tests. These distinguishing characteristics provide additional assurance regarding the identity of the isolated virus.
Prevalence of EHV-5 in horses
- The researchers also studied the prevalence of EHV-5 in a group of young racehorses using the EHV-5 specific PCR on nasopharyngeal secretions.
- The outbreak of EHV-5 in this population was estimated to be around 64%, indicating a wide spread of the virus in the studied group.
Cite This Article
APA
Bell SA, Balasuriya UB, Nordhausen RW, MacLachlan NJ.
(2006).
Isolation of equine herpesvirus-5 from blood mononuclear cells of a gelding.
J Vet Diagn Invest, 18(5), 472-475.
https://doi.org/10.1177/104063870601800509 Publication
Researcher Affiliations
- Equine Viral Disease Laboratory, Department of Pathology, Microbiology, and Immunology, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
MeSH Terms
- Animals
- DNA, Viral / chemistry
- DNA, Viral / genetics
- Gammaherpesvirinae / genetics
- Gammaherpesvirinae / isolation & purification
- Gammaherpesvirinae / ultrastructure
- Glycoproteins / chemistry
- Glycoproteins / genetics
- Herpesviridae Infections / blood
- Herpesviridae Infections / veterinary
- Herpesviridae Infections / virology
- Horse Diseases / blood
- Horse Diseases / virology
- Horses
- Male
- Microscopy, Electron, Transmission / veterinary
- Phylogeny
- Polymerase Chain Reaction / veterinary
- Viral Envelope Proteins / chemistry
- Viral Envelope Proteins / genetics
Citations
This article has been cited 8 times.- Miglinci L, Reicher P, Nell B, Koch M, Jindra C, Brandt S. Detection of Equine Papillomaviruses and Gamma-Herpesviruses in Equine Squamous Cell Carcinoma.. Pathogens 2023 Jan 23;12(2).
- Temesgen T, Getachew Y, Negussie H. Molecular Identification of Equine Herpesvirus 1, 2, and 5 in Equids with Signs of Respiratory Disease in Central Ethiopia.. Vet Med (Auckl) 2021;12:337-345.
- Anis E, Ilha MRS, Engiles JB, Wilkes RP. Evaluation of targeted next-generation sequencing for detection of equine pathogens in clinical samples.. J Vet Diagn Invest 2021 Mar;33(2):227-234.
- Van Cleemput J, Poelaert KCK, Laval K, Nauwynck HJ. Unravelling the first key steps in equine herpesvirus type 5 (EHV5) pathogenesis using ex vivo and in vitro equine models.. Vet Res 2019 Feb 18;50(1):13.
- Marenzoni ML, Stefanetti V, Danzetta ML, Timoney PJ. Gammaherpesvirus infections in equids: a review.. Vet Med (Auckl) 2015;6:91-101.
- Thorsteinsdóttir L, Torsteinsdóttir S, Svansson V. Establishment and characterization of fetal equine kidney and lung cells with extended lifespan. Susceptibility to equine gammaherpesvirus infection and transfection efficiency.. In Vitro Cell Dev Biol Anim 2016 Sep;52(8):872-7.
- Lu Z, Timoney PJ, White J, Balasuriya UB. Development of one-step TaqMan® real-time reverse transcription-PCR and conventional reverse transcription-PCR assays for the detection of equine rhinitis A and B viruses.. BMC Vet Res 2012 Jul 25;8:120.
- Marenzoni ML, Coppola G, Maranesi M, Passamonti F, Cappelli K, Capomaccio S, Verini Supplizi A, Thiry E, Coletti M. Age-dependent prevalence of equid herpesvirus 5 infection.. Vet Res Commun 2010 Dec;34(8):703-8.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
