Isolation of equine herpesvirus type 2 (equine gammaherpesvirus 2) from foals with keratoconjunctivitis.

This research paper discusses identification, isolation and treatment of ocular problems due to equine herpesvirus type 2 (EHV-2) in Thoroughbred weanling foals. The scientists successfully treated the identified ocular problems through ophthalmic medication containing idoxuridine, and confirmed the infection’s viral cause through various testing methods.

Introduction and Study Objectives

• The study involves the investigation of ocular issues characterized by conjunctivitis, epiphora, and keratopathy in 35 Thoroughbred weanling foals out of 80 that showed respiratory problems.
• The aim of the study was to identify the causative agent for the ocular problems and establish the appropriate treatment.

Methodology and Identification

• The researchers collected samples from 5 foals exhibiting the ocular symptoms and isolated equine herpesvirus type 2 (EHV-2) successfully in 3 of the cases.
• To confirm EHV-2 as the causative agent, they utilized electron microscopy, restriction endonuclease DNA fingerprinting, and Southern blot analysis. These techniques allowed them to visualize the virus, investigate its DNA structure and identify its unique pattern respectively.

Treatment and Conclusion

• After conclusively identifying EHV-2 as the cause for the ocular problems, the researchers treated the affected foals with an ophthalmic medication that contains idoxuridine. Idoxuridine is a type of antiviral drug often used in treatments of viral infections such as herpes.
• This study provides insights into the complications EHV-2 infection can play in the ocular health of young horses and emphasises the importance of early detection and application of specific antiviral treatment to manage and control potential outbreaks.