Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential.
Abstract: Mesoangioblasts (MABs) are vessel-associated stem cells that express pericyte markers and are originally isolated from the embryonic dorsal aorta. From postnatal small vessels of skeletal muscle and heart, it is possible to isolate cells with similar characteristics to embryonic MABs. Adult MABs have the capacity to self-renew and to differentiate into cell types of mesodermal lineages upon proper culture conditions. To date, the origin of MABs and the relationship with other muscle stem cells are still debated. Recently, in a phase I-II clinical trial, intra-arterial HLA-matched MABs were proved to be relatively safe. Novel information on MAB pure populations is desirable, and implementation of their therapeutic potential is mandatory to approach efficacy in MAB-based treatments. This chapter provides an overview of the current techniques for isolation and characterization of rodent, canine, human, and equine adult MABs.
Publication Date: 2021-02-13 PubMed ID: 33576976DOI: 10.1007/978-1-0716-1056-5_11Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research article focuses on exploring the nature of mesoangioblasts (MABs), which are a type of stem cells associated with vessels. MABs possess the ability to self-renew and differentiate into other cell types within certain conditions, making them of particular interest for therapeutic applications.
Understanding Mesoangioblasts
Mesoangioblasts (MABs) are a type of stem cells that are connected with vessels and demonstrate pericyte markers. They were originally isolated from the embryonic dorsal aorta, but further studies have confirmed that cells with similar characteristics can also be isolated from the small vessels of skeletal muscle and heart of postnatal mammals. These adult MABs possess the capacity to self-replenish and differentiate into other cell types of mesodermal lineage, given the right culture conditions.
- This research is crucial because the origin of MABs and their relationship with other muscle stem cells are still topics of ongoing debate in the scientific community.
Therapeutic Potential of MABs
Studies have provided empirical evidence supporting the therapeutic potential of mesoangioblasts. A phase I-II clinical trial has proved that HLA (Human Leukocyte Antigen) matched MABs, delivered intra-arterially, are relatively safe.
- Due to their capacity for self-renewal and differentiation, MABs are viewed as promising candidates for a range of potential therapeutic applications, particularly for treating various muscle and heart conditions.
- However, additional facts on pure MAB populations are needed, and plans to implement their therapeutic potential must be developed with a view to ensuring efficacy in MAB-based treatments.
Current Techniques for Isolation and Characterization of MABs
This research article provides an overview of current methodologies for isolating and characterizing adult MABs from several species including rodents, canines, humans, and horses. These methods offer essential insights for researchers seeking to better understand MABs and their role in treatment.
- The examination and understanding of the complexity of these procedures and techniques are essential for further advancements in MAB research and their potential therapeutic uses.
Cite This Article
APA
(2021).
Isolation of Mammalian Mesoangioblasts: A Subset of Pericytes with Myogenic Potential.
Methods Mol Biol, 2235, 155-167.
https://doi.org/10.1007/978-1-0716-1056-5_11 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Aorta / cytology
- Cell Differentiation / physiology
- Cell Separation / methods
- Dogs
- Horses
- Humans
- Mesoderm / cytology
- Mice
- Muscle Development
- Muscle, Skeletal / cytology
- Myoblasts / cytology
- Pericytes / cytology
- Pericytes / physiology
- Rats
- Stem Cells / cytology
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Citations
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