FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Anim Genet / Joint Report of the Fifth International Workshop on Lymphocy...
Animal genetics1988; 19(4); 447-456; doi: 10.1111/j.1365-2052.1988.tb00836.x

Joint Report of the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, Baton Rouge, Louisiana, 31 October-1 November 1987.

Abstract: Six laboratories participated in the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, testing 132 alloantisera against lymphocytes of 880 horses chosen to represent different families and breeds. Most of the alloantisera were produced by lymphocyte immunization between horses matched at the ELA-A locus. All horses were also tested with antisera contributed to the workshop by participating laboratories which identified ELA specificities A1-A10 and W12-W21. Previously identified workshop specificities ELA-W14, W15 and W19 were accepted as products of the ELA-A locus based on family and population studies by the workshop. Their designations were changed to ELA-A14, ELA-A15 and ELA-A19, respectively. Two new specificities were identified, namely ELA-W22 (W22) and ELA-W23 (W23). Population and family studies indicated that W22 and W23 as well as W13 are products of an ELA locus other than ELA-A. The presence of these specificities was correlated with the presence of certain ELA-A locus specificities, e.g. W13 with A3, W22 with A2 and W23 with A5. However, the association was not complete and W13, W22 and W23 also segregated with other ELA-A specificities in some families. Evidence for recombination was found between the ELA-A locus and the locus or loci encoding these specificities resulting in seven recombinant haplotypes found among the data presented in this workshop. Further studies are required for definitive assignment of the specificities to a class I or class II locus.
Get Full Text
Publication Date: 1988-01-01 PubMed ID: 2466424DOI: 10.1111/j.1365-2052.1988.tb00836.xGoogle Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research reports on a workshop in which six labs studied the immune system of horses, particularly lymphocyte alloantigens. The discoveries include identification of two new specificities and evidence for recombination. The analysis also suggested that there might be a relationship between these specificities and a particular locus, but further studies are needed to confirm this.

Workshop Description

  • The research article pertains to the Fifth International Workshop on Lymphocyte Alloantigens of the Horse where six laboratories conducted research on the lymphocytes and alloantisera of 880 horses selected from diverse families and breeds.
  • The main reason for the workshop was to reveal unknown patterns or relationships between the genetic makeups of different breeds and families of horses.

Research Methodology

  • Most of the alloantisera, which is a liquid that contains antibodies, were produced by injecting lymphocytes between horses matched at the ELA-A locus. A locus is a specific location of a gene on a chromosome.
  • All the horses were examined with antisera provided by the laboratories participating in the workshop. These antisera identified ELA specificities, which are essentially unique forms of a gene, from A1-A10 and W12-W21.

Findings from the Workshop

  • The pre-identified workshop specificities ELA-W14, W15 and W19 were confirmed as products of the ELA-A locus based on studies conducted among families and populations during the workshop. The designations of these specificities were subsequently changed to ELA-A14, ELA-A15 and ELA-A19 respectively.
  • Two new specificities, ELA-W22 and ELA-W23, were identified during the workshop. Studies suggested that W22, W23, and W13 are likely products of an ELA locus other than ELA-A.
  • The research showed evidence for recombination – the rearrangement of genetic material – which was found between the ELA-A locus and the locus or loci encoding these specificities. There were seven recombinant haplotypes recorded in the workshop’s data.

Next Steps from the Research

  • The presence of ELA-W22, W23 and W13 specificities was correlated with certain ELA-A locus specificities like W13 with A3, W22 with A2 and W23 with A5. However, the association was incomplete as W13, W22 and W23 also showed up with other ELA-A specificities in some families.
  • This research suggests further exploration is necessary to understand the complete relationship between these specificities and potentially assign them to class I or class II loci.

Cite This Article

APA
Lazary S, Antczak DF, Bailey E, Bell TK, Bernoco D, Byrns G, McClure JJ. (1988). Joint Report of the Fifth International Workshop on Lymphocyte Alloantigens of the Horse, Baton Rouge, Louisiana, 31 October-1 November 1987. Anim Genet, 19(4), 447-456. https://doi.org/10.1111/j.1365-2052.1988.tb00836.x

Publication

Animal genetics
ISSN: 0268-9146
NlmUniqueID: 8605704
Country: England
Language: English
Volume: 19
Issue: 4
Pages: 447-456

Researcher Affiliations

Lazary, S
  • Division of Immunogenetics, School of Veterinary Medicine, University of Berne, Switzerland.
Antczak, D F
    Bailey, E
      Bell, T K
        Bernoco, D
          Byrns, G
            McClure, J J

              MeSH Terms

              • Animals
              • Blood Grouping and Crossmatching / veterinary
              • Epitopes / genetics
              • Epitopes / immunology
              • Gene Frequency
              • Histocompatibility Antigens / analysis
              • Histocompatibility Antigens / genetics
              • Histocompatibility Antigens / immunology
              • Histocompatibility Antigens Class II / analysis
              • Histocompatibility Antigens Class II / genetics
              • Histocompatibility Antigens Class II / immunology
              • Horses / genetics
              • Horses / immunology
              • Isoantigens / analysis
              • Isoantigens / genetics
              • Isoantigens / immunology
              • Lymphocytes / immunology
              • Major Histocompatibility Complex

              Citations

              This article has been cited 23 times.
              1. Jaworska J, Tobolski D, Janowski T. Is similarity in Major Histocompatibility Complex (MHC) associated with the incidence of retained fetal membranes in draft mares? A cross-sectional study.. PLoS One 2020;15(8):e0237765.
                doi: 10.1371/journal.pone.0237765pubmed: 32804960google scholar: lookup
              2. Jeannerat E, Marti E, Thomas S, Herrera C, Sieme H, Wedekind C, Burger D. Embryo survival in the oviduct not significantly influenced by major histocompatibility complex social signaling in the horse.. Sci Rep 2020 Jan 23;10(1):1056.
                doi: 10.1038/s41598-020-58056-wpubmed: 31974438google scholar: lookup
              3. Sadeghi R, Moradi-Shahrbabak M, Miraei Ashtiani SR, Miller DC, Antczak DF. MHC haplotype diversity in Persian Arabian horses determined using polymorphic microsatellites.. Immunogenetics 2018 May;70(5):305-315.
                doi: 10.1007/s00251-017-1039-xpubmed: 29170799google scholar: lookup
              4. Miller D, Tallmadge RL, Binns M, Zhu B, Mohamoud YA, Ahmed A, Brooks SA, Antczak DF. Polymorphism at expressed DQ and DR loci in five common equine MHC haplotypes.. Immunogenetics 2017 Mar;69(3):145-156.
                doi: 10.1007/s00251-016-0964-4pubmed: 27889800google scholar: lookup
              5. Brosnahan MM, Silvela EJ, Crumb J, Miller DC, Erb HN, Antczak DF. Ectopic Trophoblast Allografts in the Horse Resist Destruction by Secondary Immune Responses.. Biol Reprod 2016 Dec;95(6):135.
                doi: 10.1095/biolreprod.115.137851pubmed: 27760752google scholar: lookup
              6. Bergmann T, Moore C, Sidney J, Miller D, Tallmadge R, Harman RM, Oseroff C, Wriston A, Shabanowitz J, Hunt DF, Osterrieder N, Peters B, Antczak DF, Sette A. The common equine class I molecule Eqca-1*00101 (ELA-A3.1) is characterized by narrow peptide binding and T cell epitope repertoires.. Immunogenetics 2015 Nov;67(11-12):675-89.
                doi: 10.1007/s00251-015-0872-zpubmed: 26399241google scholar: lookup
              7. Burger D, Dolivo G, Marti E, Sieme H, Wedekind C. Female major histocompatibility complex type affects male testosterone levels and sperm number in the horse (Equus caballus).. Proc Biol Sci 2015 May 22;282(1807):20150407.
                doi: 10.1098/rspb.2015.0407pubmed: 25904670google scholar: lookup
              8. Pezzanite LM, Fortier LA, Antczak DF, Cassano JM, Brosnahan MM, Miller D, Schnabel LV. Equine allogeneic bone marrow-derived mesenchymal stromal cells elicit antibody responses in vivo.. Stem Cell Res Ther 2015 Apr 12;6(1):54.
                doi: 10.1186/s13287-015-0053-xpubmed: 25889095google scholar: lookup
              9. Schnabel LV, Pezzanite LM, Antczak DF, Felippe MJ, Fortier LA. Equine bone marrow-derived mesenchymal stromal cells are heterogeneous in MHC class II expression and capable of inciting an immune response in vitro.. Stem Cell Res Ther 2014 Jan 24;5(1):13.
                doi: 10.1186/scrt402pubmed: 24461709google scholar: lookup
              10. Noronha LE, Huggler KE, de Mestre AM, Miller DC, Antczak DF. Molecular evidence for natural killer-like cells in equine endometrial cups.. Placenta 2012 May;33(5):379-86.
                doi: 10.1016/j.placenta.2012.01.018pubmed: 22357194google scholar: lookup
              11. Yao S, Qi J, Liu J, Chen R, Pan X, Li X, Gao F, Xia C. Expression, refolding and preliminary X-ray crystallographic analysis of equine MHC class I molecule complexed with an EIAV-Env CTL epitope.. Acta Crystallogr Sect F Struct Biol Cryst Commun 2012 Jan 1;68(Pt 1):20-3.
                doi: 10.1107/S1744309111038139pubmed: 22232164google scholar: lookup
              12. Tseng CT, Miller D, Cassano J, Bailey E, Antczak DF. Identification of equine major histocompatibility complex haplotypes using polymorphic microsatellites.. Anim Genet 2010 Dec;41 Suppl 2(Suppl 2):150-3.
                doi: 10.1111/j.1365-2052.2010.02125.xpubmed: 21070289google scholar: lookup
              13. Ramsay JD, Leib SR, Orfe L, Call DR, Tallmadge RL, Fraser DG, Mealey RH. Development of a DNA microarray for detection of expressed equine classical MHC class I sequences in a defined population.. Immunogenetics 2010 Sep;62(9):633-9.
                doi: 10.1007/s00251-010-0463-ypubmed: 20683590google scholar: lookup
              14. Tallmadge RL, Campbell JA, Miller DC, Antczak DF. Analysis of MHC class I genes across horse MHC haplotypes.. Immunogenetics 2010 Mar;62(3):159-72.
                doi: 10.1007/s00251-009-0420-9pubmed: 20099063google scholar: lookup
              15. de Mestre A, Noronha L, Wagner B, Antczak DF. Split immunological tolerance to trophoblast.. Int J Dev Biol 2010;54(2-3):445-55.
                doi: 10.1387/ijdb.082795adpubmed: 19876828google scholar: lookup
              16. Adams AP, Oriol JG, Campbell RE, Oppenheim YC, Allen WR, Antczak DF. The effect of skin allografting on the equine endometrial cup reaction.. Theriogenology 2007 Jul 15;68(2):237-47.
                doi: 10.1016/j.theriogenology.2007.04.058pubmed: 17559923google scholar: lookup
              17. Mealey RH, Lee JH, Leib SR, Littke MH, McGuire TC. A single amino acid difference within the alpha-2 domain of two naturally occurring equine MHC class I molecules alters the recognition of Gag and Rev epitopes by equine infectious anemia virus-specific CTL.. J Immunol 2006 Nov 15;177(10):7377-90.
                doi: 10.4049/jimmunol.177.10.7377pubmed: 17082657google scholar: lookup
              18. Tallmadge RL, Lear TL, Antczak DF. Genomic characterization of MHC class I genes of the horse.. Immunogenetics 2005 Nov;57(10):763-74.
                doi: 10.1007/s00251-005-0034-9pubmed: 16220348google scholar: lookup
              19. Chung C, Mealey RH, McGuire TC. Evaluation of high functional avidity CTL to Gag epitope clusters in EIAV carrier horses.. Virology 2005 Nov 25;342(2):228-39.
                doi: 10.1016/j.virol.2005.07.033pubmed: 16139857google scholar: lookup
              20. Chung C, Mealey RH, McGuire TC. CTL from EIAV carrier horses with diverse MHC class I alleles recognize epitope clusters in Gag matrix and capsid proteins.. Virology 2004 Sep 15;327(1):144-54.
                doi: 10.1016/j.virol.2004.06.035pubmed: 15327905google scholar: lookup
              21. Lonning SM, Zhang W, Leib SR, McGuire TC. Detection and induction of equine infectious anemia virus-specific cytotoxic T-lymphocyte responses by use of recombinant retroviral vectors.. J Virol 1999 Apr;73(4):2762-9.
                doi: 10.1128/JVI.73.4.2762-2769.1999pubmed: 10074123google scholar: lookup
              22. Broström H. Equine sarcoids. A clinical and epidemiological study in relation to equine leucocyte antigens (ELA).. Acta Vet Scand 1995;36(2):223-36.
                doi: 10.1186/BF03547691pubmed: 7484549google scholar: lookup
              23. Albright D, Bailey E, Woodward JG. Nucleotide sequence of a cDNA clone of the horse (Equus caballus) DRA gene.. Immunogenetics 1991;34(2):136-8.
                doi: 10.1007/BF00211427pubmed: 1869308google scholar: lookup
              Subscribe to our newsletter
              Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.