Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes.
Abstract: Using laser microdissection we prepared a set of horse chromosome arm-specific probes. Most of the probes were generated from horse chromosomes, some of them were derived from Equus zebra hartmannae. The set of probes were hybridized onto E. grevyi chromosomes in order to establish a genome-wide chromosomal correspondence between this zebra and horse. The use of arm-specific probes provided us with more information on the mutual arrangement of the genomes than we could obtain by means of whole-chromosome paints generated by flow sorting, even if we used reciprocal painting with probe sets from both species. By comparison of our results and results of comparative mapping in E. burchelli, we also established the chromosomal correspondence between E. grevyi and E. burchelli, providing evidence for a very close karyotypic relationship between these two zebra species. Establishment of the comparative map for E. grevyi contributes to the knowledge of the karyotypic phylogeny in the Equidae family.
Publication Date: 2007-08-23 PubMed ID: 17874215DOI: 10.1007/s10577-007-1164-8Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article investigates the genetic relationships among the Grevy’s zebra (Equus grevyi), the plains zebra (Equus burchelli), and domestic horses by using specific chromosome arm probes. The study reveals a close genealogical relationship between the two zebra species, contributing to our understanding of genetic evolution in the Equidae family.
Methodology
- The researchers used a technique called laser microdissection to prepare a variety of probes specific to different horse chromosome arms.
- Some of the probes came from horse chromosomes, while a few were derived from a zebra species, specifically Equus zebra hartmannae.
- They then applied these probes onto the chromosomes of the Grevy’s zebra (E. grevyi).
Findings
- This enabled the scientists to draw a genome-wide chromosomal correlation or correspondence between the zebra (E. grevyi) and a domestic horse.
- By using such arm-specific probes, the researchers could obtain more detailed information about the genomes’ mutual arrangement than through earlier methods, such as the use of whole-chromosome paints generated by flow sorting.
- This approach also remained effective even if reciprocal painting was conducted using probe sets from both species.
Comparison and Conclusion
- The researchers compared their findings with results of comparative mapping in the Plains zebra (E. burchelli).
- This comparison allowed the establishment of the chromosomal correspondence between the Grevy’s zebra and the Plains zebra, indicating a close karyotypic (relating to the chromosomes) relationship between these two zebra species.
- Finally, by delineating this comparative map for E. grevyi, the research contributes to the broader knowledge of chromosomal evolution and karyotypic phylogeny in the Equidae family, which includes horses, donkeys, and zebras.
Cite This Article
APA
Musilova P, Kubickova S, Zrnova E, Horin P, Vahala J, Rubes J.
(2007).
Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes.
Chromosome Res, 15(6), 807-813.
https://doi.org/10.1007/s10577-007-1164-8 Publication
Researcher Affiliations
- Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic. musilova@vri.cz
MeSH Terms
- Animals
- Chromosome Banding
- Chromosome Mapping
- Chromosome Painting
- Chromosomes / ultrastructure
- DNA Probes / chemistry
- Equidae
- Horses
- Karyotyping
- Metaphase
- Models, Genetic
- Nucleic Acid Hybridization
- Species Specificity
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Citations
This article has been cited 6 times.- Musilova P, Drbalova J, Kubickova S, Cernohorska H, Stepanova H, Rubes J. Illegitimate recombination between T cell receptor genes in humans and pigs (Sus scrofa domestica).. Chromosome Res 2014 Dec;22(4):483-93.
- Musilova P, Kubickova S, Vahala J, Rubes J. Subchromosomal karyotype evolution in Equidae.. Chromosome Res 2013 Apr;21(2):175-87.
- Piras FM, Nergadze SG, Magnani E, Bertoni L, Attolini C, Khoriauli L, Raimondi E, Giulotto E. Uncoupling of satellite DNA and centromeric function in the genus Equus.. PLoS Genet 2010 Feb 12;6(2):e1000845.
- Musilova P, Kubickova S, Horin P, Vodicka R, Rubes J. Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes.. Chromosome Res 2009;17(6):783-90.
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