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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2007; 15(6); 807-813; doi: 10.1007/s10577-007-1164-8

Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes.

Abstract: Using laser microdissection we prepared a set of horse chromosome arm-specific probes. Most of the probes were generated from horse chromosomes, some of them were derived from Equus zebra hartmannae. The set of probes were hybridized onto E. grevyi chromosomes in order to establish a genome-wide chromosomal correspondence between this zebra and horse. The use of arm-specific probes provided us with more information on the mutual arrangement of the genomes than we could obtain by means of whole-chromosome paints generated by flow sorting, even if we used reciprocal painting with probe sets from both species. By comparison of our results and results of comparative mapping in E. burchelli, we also established the chromosomal correspondence between E. grevyi and E. burchelli, providing evidence for a very close karyotypic relationship between these two zebra species. Establishment of the comparative map for E. grevyi contributes to the knowledge of the karyotypic phylogeny in the Equidae family.
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Publication Date: 2007-08-23 PubMed ID: 17874215DOI: 10.1007/s10577-007-1164-8Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article investigates the genetic relationships among the Grevy’s zebra (Equus grevyi), the plains zebra (Equus burchelli), and domestic horses by using specific chromosome arm probes. The study reveals a close genealogical relationship between the two zebra species, contributing to our understanding of genetic evolution in the Equidae family.

Methodology

  • The researchers used a technique called laser microdissection to prepare a variety of probes specific to different horse chromosome arms.
  • Some of the probes came from horse chromosomes, while a few were derived from a zebra species, specifically Equus zebra hartmannae.
  • They then applied these probes onto the chromosomes of the Grevy’s zebra (E. grevyi).

Findings

  • This enabled the scientists to draw a genome-wide chromosomal correlation or correspondence between the zebra (E. grevyi) and a domestic horse.
  • By using such arm-specific probes, the researchers could obtain more detailed information about the genomes’ mutual arrangement than through earlier methods, such as the use of whole-chromosome paints generated by flow sorting.
  • This approach also remained effective even if reciprocal painting was conducted using probe sets from both species.

Comparison and Conclusion

  • The researchers compared their findings with results of comparative mapping in the Plains zebra (E. burchelli).
  • This comparison allowed the establishment of the chromosomal correspondence between the Grevy’s zebra and the Plains zebra, indicating a close karyotypic (relating to the chromosomes) relationship between these two zebra species.
  • Finally, by delineating this comparative map for E. grevyi, the research contributes to the broader knowledge of chromosomal evolution and karyotypic phylogeny in the Equidae family, which includes horses, donkeys, and zebras.

Cite This Article

APA
Musilova P, Kubickova S, Zrnova E, Horin P, Vahala J, Rubes J. (2007). Karyotypic relationships among Equus grevyi, Equus burchelli and domestic horse defined using horse chromosome arm-specific probes. Chromosome Res, 15(6), 807-813. https://doi.org/10.1007/s10577-007-1164-8

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 15
Issue: 6
Pages: 807-813

Researcher Affiliations

Musilova, P
  • Department of Genetics and Reproduction, Veterinary Research Institute, Brno, Czech Republic. musilova@vri.cz
Kubickova, S
    Zrnova, E
      Horin, P
        Vahala, J
          Rubes, J

            MeSH Terms

            • Animals
            • Chromosome Banding
            • Chromosome Mapping
            • Chromosome Painting
            • Chromosomes / ultrastructure
            • DNA Probes / chemistry
            • Equidae
            • Horses
            • Karyotyping
            • Metaphase
            • Models, Genetic
            • Nucleic Acid Hybridization
            • Species Specificity

            References

            This article includes 28 references
            1. Chaudhary R, Raudsepp T, Guan XY, Zhang H, Chowdhary BP. Zoo-FISH with microdissected arm specific paints for HSA2, 5, 6, 16, and 19 refines known homology with pig and horse chromosomes.. Mamm Genome 1998 Jan;9(1):44-9.
              pubmed: 9434944doi: 10.1007/s003359900677google scholar: lookup
            2. Richard F, Messaoudi C, Lombard M, Dutrillaux B. Chromosome homologies between man and mountain zebra (Equus zebra hartmannae) and description of a new ancestral synteny involving sequences homologous to human chromosomes 4 and 8.. Cytogenet Cell Genet 2001;93(3-4):291-6.
              pubmed: 11528128doi: 10.1159/000057000google scholar: lookup
            3. Andersson L, Archibald A, Ashburner M, Audun S, Barendse W, Bitgood J, Bottema C, Broad T, Brown S, Burt D, Charlier C, Copeland N, Davis S, Davisson M, Edwards J, Eggen A, Elgar G, Eppig JT, Franklin I, Grewe P, Gill T 3rd, Graves JA, Hawken R, Hetzel J, Womack J. Comparative genome organization of vertebrates. The First International Workshop on Comparative Genome Organization.. Mamm Genome 1996 Oct;7(10):717-34.
              pubmed: 8854859doi: 10.1007/s003359900222google scholar: lookup
            4. Rubes J, Pagacova E, Kopecna O, Kubickova S, Cernohorska H, Vahala J, Di Berardino D. Karyotype, centric fusion polymorphism and chromosomal aberrations in captive-born mountain reedbuck (Redunca fulvorufula).. Cytogenet Genome Res 2007;116(4):263-8.
              pubmed: 17431324doi: 10.1159/000100410google scholar: lookup
            5. Meltzer PS, Guan XY, Burgess A, Trent JM. Rapid generation of region specific probes by chromosome microdissection and their application.. Nat Genet 1992 Apr;1(1):24-8.
              pubmed: 1301994doi: 10.1038/ng0492-24google scholar: lookup
            6. Telenius H, Pelmear AH, Tunnacliffe A, Carter NP, Behmel A, Ferguson-Smith MA, Nordenskjöld M, Pfragner R, Ponder BA. Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes.. Genes Chromosomes Cancer 1992 Apr;4(3):257-63.
              pubmed: 1382568doi: 10.1002/gcc.2870040311google scholar: lookup
            7. Ryder OA, Epel NC, Benirschke K. Chromosome banding studies of the Equidae.. Cytogenet Cell Genet 1978;20(1-6):332-50.
              pubmed: 648186
            8. Yang F, Fu B, O'Brien PC, Robinson TJ, Ryder OA, Ferguson-Smith MA. Karyotypic relationships of horses and zebras: results of cross-species chromosome painting.. Cytogenet Genome Res 2003;102(1-4):235-43.
              pubmed: 14970709doi: 10.1159/000075755google scholar: lookup
            9. Kubickova S, Cernohorska H, Musilova P, Rubes J. The use of laser microdissection for the preparation of chromosome-specific painting probes in farm animals.. Chromosome Res 2002;10(7):571-7.
              pubmed: 12498346doi: 10.1023/a:1020914702767google scholar: lookup
            10. Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Committee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996.. Chromosome Res 1997 Nov;5(7):433-43.
              pubmed: 9421259doi: 10.1023/a:1018408811881google scholar: lookup
            11. Pinton A, Ducos A, Yerle M. Chromosomal rearrangements in cattle and pigs revealed by chromosome microdissection and chromosome painting.. Genet Sel Evol 2003 Nov-Dec;35(6):685-96.
              pubmed: 14604515doi: 10.1186/1297-9686-35-7-685google scholar: lookup
            12. Seabright M. A rapid banding technique for human chromosomes.. Lancet 1971 Oct 30;2(7731):971-2.
              pubmed: 4107917doi: 10.1016/s0140-6736(71)90287-xgoogle scholar: lookup
            13. George M Jr, Ryder OA. Mitochondrial DNA evolution in the genus Equus.. Mol Biol Evol 1986 Nov;3(6):535-46.
              pubmed: 2832696doi: 10.1093/oxfordjournals.molbev.a040414google scholar: lookup
            14. Mrasek K, Heller A, Rubtsov N, Trifonov V, Starke H, Claussen U, Liehr T. Detailed Hylobates lar karyotype defined by 25-color FISH and multicolor banding.. Int J Mol Med 2003 Aug;12(2):139-46.
              pubmed: 12851708
            15. Scherthan H, Cremer T, Arnason U, Weier HU, Lima-de-Faria A, Frönicke L. Comparative chromosome painting discloses homologous segments in distantly related mammals.. Nat Genet 1994 Apr;6(4):342-7.
              pubmed: 8054973doi: 10.1038/ng0494-342google scholar: lookup
            16. Goureau A, Yerle M, Schmitz A, Riquet J, Milan D, Pinton P, Frelat G, Gellin J. Human and porcine correspondence of chromosome segments using bidirectional chromosome painting.. Genomics 1996 Sep 1;36(2):252-62.
              pubmed: 8812451doi: 10.1006/geno.1996.0460google scholar: lookup
            17. Mrasek K, Heller A, Rubtsov N, Trifonov V, Starke H, Rocchi M, Claussen U, Liehr T. Reconstruction of the female Gorilla gorilla karyotype using 25-color FISH and multicolor banding (MCB).. Cytogenet Cell Genet 2001;93(3-4):242-8.
              pubmed: 11528119doi: 10.1159/000056991google scholar: lookup
            18. Wienberg J. The evolution of eutherian chromosomes.. Curr Opin Genet Dev 2004 Dec;14(6):657-66.
              pubmed: 15531161doi: 10.1016/j.gde.2004.10.001google scholar: lookup
            19. Chowdhary BP, Raudsepp T, Frönicke L, Scherthan H. Emerging patterns of comparative genome organization in some mammalian species as revealed by Zoo-FISH.. Genome Res 1998 Jun;8(6):577-89.
              pubmed: 9647633doi: 10.1101/gr.8.6.577google scholar: lookup
            20. Gross M, Starke H, Trifonov V, Claussen U, Liehr T, Weise A. A molecular cytogenetic study of chromosome evolution in chimpanzee.. Cytogenet Genome Res 2006;112(1-2):67-75.
              pubmed: 16276092doi: 10.1159/000087515google scholar: lookup
            21. Yang F, Fu B, O'Brien PC, Nie W, Ryder OA, Ferguson-Smith MA. Refined genome-wide comparative map of the domestic horse, donkey and human based on cross-species chromosome painting: insight into the occasional fertility of mules.. Chromosome Res 2004;12(1):65-76.
              pubmed: 14984103doi: 10.1023/b:chro.0000009298.02689.8agoogle scholar: lookup
            22. Goh G, Raudsepp T, Durkin K, Wagner ML, Schäffer AA, Agarwala R, Tozaki T, Mickelson JR, Chowdhary BP. High-resolution gene maps of horse chromosomes 14 and 21: additional insights into evolution and rearrangements of HSA5 homologs in mammals.. Genomics 2007 Jan;89(1):89-112.
              pubmed: 16916595doi: 10.1016/j.ygeno.2006.06.012google scholar: lookup
            23. Brinkmeyer-Langford C, Raudsepp T, Lee EJ, Goh G, Schäffer AA, Agarwala R, Wagner ML, Tozaki T, Skow LC, Womack JE, Mickelson JR, Chowdhary BP. A high-resolution physical map of equine homologs of HSA19 shows divergent evolution compared with other mammals.. Mamm Genome 2005 Aug;16(8):631-49.
              pubmed: 16180145doi: 10.1007/s00335-005-0023-1google scholar: lookup
            24. Raudsepp T, Chowdhary BP. Construction of chromosome-specific paints for meta- and submetacentric autosomes and the sex chromosomes in the horse and their use to detect homologous chromosomal segments in the donkey.. Chromosome Res 1999;7(2):103-14.
              pubmed: 10328622doi: 10.1023/a:1009234814635google scholar: lookup
            25. Wijers ER, Zijlstra C, Lenstra JA. Rapid evolution of horse satellite DNA.. Genomics 1993 Oct;18(1):113-7.
              pubmed: 8276394doi: 10.1006/geno.1993.1433google scholar: lookup
            26. Trifonov V, Yang F, Ferguson-Smith MA, Robinson TJ. Cross-species chromosome painting in the Perissodactyla: delimitation of homologous regions in Burchell's zebra (Equus Burchellii) and the white (Ceratotherium Simum) and black rhinoceros (Diceros Bicornis).. Cytogenet Genome Res 2003;103(1-2):104-10.
              pubmed: 15004472doi: 10.1159/000076297google scholar: lookup
            27. Carbone L, Nergadze SG, Magnani E, Misceo D, Francesca Cardone M, Roberto R, Bertoni L, Attolini C, Francesca Piras M, de Jong P, Raudsepp T, Chowdhary BP, Guérin G, Archidiacono N, Rocchi M, Giulotto E. Evolutionary movement of centromeres in horse, donkey, and zebra.. Genomics 2006 Jun;87(6):777-82.
              pubmed: 16413164doi: 10.1016/j.ygeno.2005.11.012google scholar: lookup
            28. Schermelleh L, Thalhammer S, Heckl W, Pösl H, Cremer T, Schütze K, Cremer M. Laser microdissection and laser pressure catapulting for the generation of chromosome-specific paint probes.. Biotechniques 1999 Aug;27(2):362-7.
              pubmed: 10457845doi: 10.2144/99272rr04google scholar: lookup

            Citations

            This article has been cited 8 times.
            1. Musilova P, Drbalova J, Kubickova S, Cernohorska H, Stepanova H, Rubes J. Illegitimate recombination between T cell receptor genes in humans and pigs (Sus scrofa domestica). Chromosome Res 2014 Dec;22(4):483-93.
              doi: 10.1007/s10577-014-9434-8pubmed: 25038896google scholar: lookup
            2. Musilova P, Kubickova S, Vahala J, Rubes J. Subchromosomal karyotype evolution in Equidae. Chromosome Res 2013 Apr;21(2):175-87.
              doi: 10.1007/s10577-013-9346-zpubmed: 23532666google scholar: lookup
            3. Piras FM, Nergadze SG, Magnani E, Bertoni L, Attolini C, Khoriauli L, Raimondi E, Giulotto E. Uncoupling of satellite DNA and centromeric function in the genus Equus. PLoS Genet 2010 Feb 12;6(2):e1000845.
              doi: 10.1371/journal.pgen.1000845pubmed: 20169180google scholar: lookup
            4. Musilova P, Kubickova S, Horin P, Vodicka R, Rubes J. Karyotypic relationships in Asiatic asses (kulan and kiang) as defined using horse chromosome arm-specific and region-specific probes. Chromosome Res 2009;17(6):783-90.
              doi: 10.1007/s10577-009-9069-3pubmed: 19731053google scholar: lookup
            5. Trifonov VA, Stanyon R, Nesterenko AI, Fu B, Perelman PL, O'Brien PC, Stone G, Rubtsova NV, Houck ML, Robinson TJ, Ferguson-Smith MA, Dobigny G, Graphodatsky AS, Yang F. Multidirectional cross-species painting illuminates the history of karyotypic evolution in Perissodactyla. Chromosome Res 2008;16(1):89-107.
              doi: 10.1007/s10577-007-1201-7pubmed: 18293107google scholar: lookup
            6. Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
              doi: 10.1007/s10577-008-1204-zpubmed: 18274866google scholar: lookup
            7. Flack N, Hughes L, Cassens J, Enriquez M, Gebeyehu S, Alshagawi M, Hatfield J, Kauffman A, Brown B, Klaeui C, Mabrouk IF, Walls C, Yeater T, Rivas A, Faulk C. The genome of Przewalski's horse (Equus ferus przewalskii). G3 (Bethesda) 2024 Aug 7;14(8).
              doi: 10.1093/g3journal/jkae113pubmed: 38805182google scholar: lookup
            8. Flack N, Hughes L, Cassens J, Enriquez M, Gebeyehu S, Alshagawi M, Hatfield J, Kauffman A, Brown B, Klaeui C, Mabrouk IF, Walls C, Yeater T, Rivas A, Faulk C. The genome of Przewalski's horse (Equus ferus przewalskii). bioRxiv 2024 Feb 28;.
              doi: 10.1101/2024.02.20.581252pubmed: 38464182google scholar: lookup
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