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Home / Equine Research Bank / Animals (Basel) / Laissez-Faire Stallions? Males’ Fecal Cortisol Metabol...
Animals : an open access journal from MDPI2023; 13(1); doi: 10.3390/ani13010176

Laissez-Faire Stallions? Males’ Fecal Cortisol Metabolite Concentrations Do Not Vary with Increased Female Turnover in Feral Horses (Equus caballus).

Abstract: Stress responses can be triggered by several physical and social factors, prompting physiological reactions including increases in glucocorticoid concentrations. In a population of feral horses (Equus caballus) on Shackleford Banks, North Carolina, females previously immunized with the immunocontraceptive agent porcine zona pellucida (PZP) change social groups (bands) more often than unimmunized females, disrupting the social stability within the population. We assessed the effects of increased female group changing behavior (or female turnover) on individual male stress by comparing fecal cortisol metabolite (FCM) concentrations among stallions experiencing varying amounts of female group changing behavior. FCM concentrations did not significantly correlate with female turnover. Similarly, FCM concentrations were not dependent upon the timing of female group changing behavior. These findings suggest that female turnover rate has little influence on physiological measures of stress in associated stallions. That said, Shackleford stallions experiencing increased female turnover do engage in behaviors typically associated with stress (increased vigilance, highly escalated male-male conflicts). Future work should compare FCM concentrations across time within populations and among populations managed under different strategies to better isolate factors influencing stallion stress physiology. Such studies are especially important if we are to determine how changes in female behavior related to immunocontraception impact physiological and behavioral indicators of stress for non-target animals. Finally, our study highlights the importance of considering both physiological and behavioral measures when investigating animal responses to potentially challenging situations.
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Publication Date: 2023-01-03 PubMed ID: 36611784PubMed Central: PMC9817692DOI: 10.3390/ani13010176Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates whether the disruptive behavior of female horses, particularly their frequent changes in social groups, impacts the stress levels of male horses, measured through cortisol levels in their feces. Surprisingly, the study finds no correlation between female behavior and cortisol levels in males, hinting that males do not physiologically stress over such disruptions.

Introduction and Methodology

  • The study was conducted on a population of feral horses on Shackleford Banks, North Carolina.
  • In this population, it was observed that females who had been immunized with the contraceptive agent porcine zona pellucida (PZP) changed social groups more frequently than those who had not been immunized.
  • This behavior was seen to disrupt the social stability of the population.
  • The researchers sought to understand whether this increased female group changing behavior, or female turnover, had any effect on the stress levels of the individual male horses.
  • They measured the stress by comparing the levels of cortisol metabolite in the feces of stallions who were experiencing different amounts of female group changing behavior.

Results

  • The results revealed that the fecal cortisol metabolite (FCM) concentrations did not significantly correlate with female turnover.
  • In other words, the stress levels of the stallions did not seem to increase as a result of frequent changes in the female social groups.
  • Additionally, there was no significant change in FCM concentrations based on the timing of female group changing behavior.

Implications and Future Directions

  • Despite the lack of physiological stress indicators, the stallions did show behavioral changes like increased vigilance and escalated male-male conflicts which are typically associated with stress.
  • This hints that changes in female social groups do affect stallions, but this effect is not reflected in cortisol levels.
  • Future research should focus on comparing FCM concentrations across time within and among populations managed under different strategies.
  • It is crucial to understand how changes in female behavior, especially those associated with immunocontraception, can impact the stress indicators of non-target animals.
  • The study underlines the significance of considering both physiological and behavioral measures when studying animal responses to challenging situations.

Cite This Article

APA
Jones MM, Nuñez CMV. (2023). Laissez-Faire Stallions? Males’ Fecal Cortisol Metabolite Concentrations Do Not Vary with Increased Female Turnover in Feral Horses (Equus caballus). Animals (Basel), 13(1). https://doi.org/10.3390/ani13010176

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 1

Researcher Affiliations

Jones, Maggie M
  • Department of Natural Resource Ecology and Management, Iowa State University, 2310 Pammel Dr., Ames, IA 50011, USA.
  • School of Natural Resources and Environment, University of Florida, P.O. Box 116455, Building 0724, 103 Black Hall, Gainesville, FL 32611, USA.
Nuñez, Cassandra M V
  • Department of Biological Sciences, The University of Memphis, 3774 Walker Avenue, Ellington Hall, Room 239, Memphis, TN 38512, USA.

Grant Funding

  • 1744592 / National Science Foundation
  • project IOW05509 / USDA National Institute of Food and Agriculture Hatch Multistate Funding

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

References

This article includes 71 references
  1. Creel S, Dantzer B, Goymann W, Rubenstein D.R. The ecology of stress: Effects of the social environment.. Funct. Ecol. 2013;27:66–80.
    doi: 10.1111/j.1365-2435.2012.02029.xgoogle scholar: lookup
  2. Mostl E, Palme R. Hormones as indicators of stress.. Domest. Anim. Endocrinol. 2002;23:67–74.
    doi: 10.1016/S0739-7240(02)00146-7pubmed: 12142227google scholar: lookup
  3. Sapolsky R.M. The influence of social hierarchy on primate health.. Science 2005;308:648–652.
    doi: 10.1126/science.1106477pubmed: 15860617google scholar: lookup
  4. Blas J, Bortolotti G.R, Tella J.L, Baos R, Marchant T.A. Stress response during development predicts fitness in a wild, long lived vertebrate.. Proc. Natl. Acad. Sci. USA 2007;104:8880–8884.
    doi: 10.1073/pnas.0700232104pmc: PMC1868653pubmed: 17517658google scholar: lookup
  5. Rakotoniaina J.H, Kappeler P.M, Kaesler E, Hämäläinen A.M, Kirschbaum C, Kraus C. Hair cortisol concentrations correlate negatively with survival in a wild primate population.. BMC Ecol. 2017;17:30.
    doi: 10.1186/s12898-017-0140-1pmc: PMC5579956pubmed: 28859635google scholar: lookup
  6. Schoenle L.A, Zimmer C, Vitousek M.N. Understanding context dependence in glucocorticoid–fitness relationships: The role of the nature of the challenge, the intensity and frequency of stressors, and life history.. Integr. Comp. Biol. 2018;58:777–789.
    doi: 10.1093/icb/icy046pubmed: 29889246google scholar: lookup
  7. Beehner J.C, Bergman T.J. The next step for stress research in primates: To identify relationships between glucocorticoid secretion and fitness.. Horm. Behav. 2017;91:68–83.
    doi: 10.1016/j.yhbeh.2017.03.003pubmed: 28284709google scholar: lookup
  8. Boonstra R. Reality as the leading cause of stress: Rethinking the impact of chronic stress in nature.. Funct. Ecol. 2013;27:11–23.
    doi: 10.1111/1365-2435.12008google scholar: lookup
  9. Feist J.D, McCullough D.R. Behavior patterns and communication in feral horses.. Z. Für Tierpsychol. 1976;41:337–371.
    doi: 10.1111/j.1439-0310.1976.tb00947.xpubmed: 983427google scholar: lookup
  10. Rubenstein D.I. Behavioural ecology of island feral horses.. Equine Vet. J. 1981;13:27–34.
    doi: 10.1111/j.2042-3306.1981.tb03443.xgoogle scholar: lookup
  11. Klingel H. Social organization and reproduction in equids.. J. Reprod. Fertil. 1975;23:7–11.
    pubmed: 1060868
  12. Madosky J.M, Rubenstein D.I, Howard J.J, Stuska S. The effects of immunocontraception on harem fidelity in a feral horse (Equus caballus) population.. Appl. Anim. Behav. Sci. 2010;128:50–56.
    doi: 10.1016/j.applanim.2010.09.013google scholar: lookup
  13. Nuñez C.M.V, Adelman J.S, Mason C, Rubenstein D.I. Immunocontraception decreases group fidelity in a feral horse population during the non-breeding season.. Appl. Anim. Behav. Sci. 2009;117:74–83.
    doi: 10.1016/j.applanim.2008.12.001google scholar: lookup
  14. Ransom J.I, Cade B.S, Hobbs N.T. Influences of immunocontraception on time budgets, social behavior, and body condition in feral horses.. Appl. Anim. Behav. Sci. 2010;124:51–60.
    doi: 10.1016/j.applanim.2010.01.015google scholar: lookup
  15. Jones M.M, Nuñez C.M.V. Decreased female fidelity alters male behavior in a feral horse population managed with immunocontraception.. Appl. Anim. Behav. Sci. 2019;214:34–41.
    doi: 10.1016/j.applanim.2019.03.005google scholar: lookup
  16. Nuñez C.M.V, Adelman J.S, Carr H.A, Alvarez C.M, Rubenstein D.I. Lingering effects of contraception management on feral mare (Equus caballus) fertility and social behavior.. Conserv. Physiol. 2017;5:cox018.
    doi: 10.1093/conphys/cox018pmc: PMC6007543pubmed: 29977561google scholar: lookup
  17. Nuñez C.M.V, Adelman J.S, Smith J, Gesquiere L.R, Rubenstein D.I. Linking social environment and stress physiology in feral mares (Equus caballus): Group transfers elevate fecal cortisol levels.. Gen. Comp. Endocrinol. 2014;196:26–33.
    doi: 10.1016/j.ygcen.2013.11.012pubmed: 24275609google scholar: lookup
  18. Arlet M.E, Grote M.N, Molleman F, Isbell L.A, Carey J.R. Reproductive tactics influence cortisol levels in individual male gray-cheeked mangabeys (Lophocebus albigena). Horm. Behav. 2009;55:210–216.
    doi: 10.1016/j.yhbeh.2008.10.004pubmed: 18996388google scholar: lookup
  19. Lynch J.W, Ziegler T.E, Strier K.B. Individual and seasonal variation in fecal testosterone and cortisol levels of wild male tufted capuchin monkeys, Cebus apella nigritus.. Horm. Behav. 2002;41:275–287.
    doi: 10.1006/hbeh.2002.1772pubmed: 11971661google scholar: lookup
  20. Sands J, Creel S. Social dominance, aggression and faecal glucocorticoid levels in a wild population of wolves, Canis lupus.. Anim. Behav. 2004;67:387–396.
    doi: 10.1016/j.anbehav.2003.03.019google scholar: lookup
  21. Nuñez C.M.V, Adelman J.S, Rubenstein D.I. Immunocontraception in wild horses (Equus caballus) extends reproductive cycling beyond the normal breeding season.. PLoS ONE 2010;5:e13635.
    doi: 10.1371/journal.pone.0013635pmc: PMC2964306pubmed: 21049017google scholar: lookup
  22. Bro-Jørgensen J. Intra-and intersexual conflicts and cooperation in the evolution of mating strategies: Lessons learnt from ungulates.. Evol. Biol. 2011;38:28–41.
    doi: 10.1007/s11692-010-9105-4google scholar: lookup
  23. Wong B.B, Candolin U. How is female mate choice affected by male competition?. Biol. Rev. 2005;80:559–571.
    doi: 10.1017/S1464793105006809pubmed: 16221329google scholar: lookup
  24. Rubenstein D.I. Ecology and sociality in horses and zebras.. 1986. pp. 282–302.
  25. Huhman K.L, Moore T.O, Ferris C.F, Mougey E.H, Meyerhoff J.L. Acute and repeated exposure to social conflict in male golden hamsters: Increases in plasma POMC-peptides and cortisol and decreases in plasma testosterone.. Horm. Behav. 1991;25:206–216.
    doi: 10.1016/0018-506X(91)90051-Ipubmed: 1648544google scholar: lookup
  26. Muller M.N, Wrangham R.W. Dominance, cortisol and stress in wild chimpanzees (Pan troglodytes schweinfurthii). Behav. Ecol. Sociobiol. 2004;55:332–340.
    doi: 10.1007/s00265-003-0713-1pmc: PMC7990237pubmed: 33776193google scholar: lookup
  27. Øverli Ø, Harris C.A, Winberg S. Short-term effects of fights for social dominance and the establishment of dominant-subordinate relationships on brain monoamines and cortisol in rainbow trout.. Brain Behav. Evol. 1999;54:263–275.
    doi: 10.1159/000006627pubmed: 10640786google scholar: lookup
  28. Sheriff M, Wheeler H, Donker S, Krebs C, Palme R, Hik D, Boonstra R. Mountain-top and valley-bottom experiences: The stress axis as an integrator of environmental variability in arctic ground squirrel populations.. J. Zool. 2012;287:65–75.
    doi: 10.1111/j.1469-7998.2011.00888.xgoogle scholar: lookup
  29. . Cape Lookout NS (CALO) Special Report.. 2018.
  30. . Shackleford Banks Wild Horses Protection Act.. H.R. 765, 105th Congress; Washington, DC, USA: 1997. p. 6.
  31. Altmann J. Observational study of behavior: Sampling methods.. Behaviour 1974;49:227–267.
    doi: 10.1163/156853974X00534pubmed: 4597405google scholar: lookup
  32. Hynes C.A. Male-Male Aggression and Dominance in a Disturbed Population of Feral Horses.. 1998.
  33. . Horses of Shackleford Banks.. 2004.
  34. Aben J, Pellikka P, Travis J.M. A call for viewshed ecology: Advancing our understanding of the ecology of information through viewshed analysis.. Methods Ecol. Evol. 2018;9:624–633.
    doi: 10.1111/2041-210X.12902google scholar: lookup
  35. Alonso J.C, Álvarez-Martínez J.M, Palacín C. Leks in ground-displaying birds: Hotspots or safe places?. Behav. Ecol. 2012;23:491–501.
    doi: 10.1093/beheco/arr215google scholar: lookup
  36. . USGS Lidar Point Cloud NC Sandy-L15 2014; U.S. Geological Survey, 2015.. .
  37. Palme R, Fischer P, Schildorfer H, Ismail M. Excretion of infused 14C-steroid hormones via faeces and urine in domestic livestock.. Anim. Reprod. Sci. 1996;43:43–63.
    doi: 10.1016/0378-4320(95)01458-6google scholar: lookup
  38. Khan M.Z, Altmann J, Isani S.S, Yu J. A matter of time: Evaluating the storage of fecal samples for steroid analysis.. Gen. Comp. Endocrinol. 2002;128:57–64.
    doi: 10.1016/S0016-6480(02)00063-1pubmed: 12270788google scholar: lookup
  39. Kozlowski C.P, Clawitter H.L, Thier T, Fischer M.T, Asa C.S. Characterization of estrous cycles and pregnancy in Somali wild asses (Equus africanus somaliensis) through fecal hormone analyses.. Zoo Biol. 2018;37:35–39.
    doi: 10.1002/zoo.21397pubmed: 29377248google scholar: lookup
  40. Beehner J.C, Gesquiere L, Seyfarth R.M, Cheney D.L, Alberts S.C, Altmann J. Testosterone related to age and life-history stages in male baboons and geladas.. Horm. Behav. 2009;56:472–480.
    doi: 10.1016/j.yhbeh.2009.08.005pmc: PMC3630238pubmed: 19712676google scholar: lookup
  41. Hunt K.E, Wasser S.K. Effect of long-term preservation methods on fecal glucocorticoid concentrations of grizzly bear and African elephant.. Physiol. Biochem. Zool. 2003;76:918–928.
    doi: 10.1086/380209pubmed: 14988807google scholar: lookup
  42. Lynch J, Khan M, Altmann J, Njahira M, Rubenstein N. Concentrations of four fecal steroids in wild baboons: Short-term storage conditions and consequences for data interpretation.. Gen. Comp. Endocrinol. 2003;132:264–271.
    doi: 10.1016/S0016-6480(03)00093-5pubmed: 12812774google scholar: lookup
  43. Brown J, Walker S, Steinman K. Endocrine manual for the reproductive assessment of domestic and non-domestic species.. Endocr. Res. Lab. Dep. Reprod. Sci. Conserv. Res. Cent. Natl. Zool. Park Smithson. Inst. Handb. 2004;1:93.
  44. Robbins C.T. Wildlife Feeding and Nutrition.. 2nd ed. Academic Press; New York, NY, USA: 1993.
  45. Goymann W, East M.L, Wachter B, Höner O.P, Möstl E, Van’t Hof T.J, Hofer H. Social, state-dependent and environmental modulation of faecal corticosteroid levels in free-ranging female spotted hyenas.. Proc. R. Soc. B Biol. Sci. 2001;268:2453–2459.
    doi: 10.1098/rspb.2001.1828pmc: PMC1088899pubmed: 11747563google scholar: lookup
  46. Vera F, Zenuto R.R, Antenucci C.D. Seasonal variations in plasma cortisol, testosterone, progesterone and leukocyte profiles in a wild population of tuco-tucos.. J. Zool. 2013;289:111–118.
    doi: 10.1111/j.1469-7998.2012.00967.xgoogle scholar: lookup
  47. Rudman R, Keiper R.R. The body condition of feral ponies on Assateague Island.. Equine Vet. J. 1991;23:453–456.
    doi: 10.1111/j.2042-3306.1991.tb03760.xpubmed: 1778164google scholar: lookup
  48. R Core Team. R: A Language and Environment for Statistical Computing, 3.6.1.. R Foundation for Statistical Computing; Vienna, Austria: 2019.
  49. Berger J. Organizational systems and dominance in feral horses in Grand Canyon.. Behav. Ecol. Sociobiol. 1977;2:131–146.
    doi: 10.1007/BF00361898google scholar: lookup
  50. Ruis M.A, Te Brake J.H, Engel B, Ekkel E.D, Buist W.G, Blokhuis H.J, Koolhaas J.M. The circadian rhythm of salivary cortisol in growing pigs: Effects of age, gender, and stress.. Physiol. Behav. 1997;62:623–630.
    doi: 10.1016/S0031-9384(97)00177-7pubmed: 9272674google scholar: lookup
  51. Vervaecke H, Stevens J.M, Vandemoortele H, Sigurjónsdóttir H, De Vries H. Aggression and dominance in matched groups of subadult Icelandic horses (Equus caballus). J. Ethol. 2007;25:239–248.
    doi: 10.1007/s10164-006-0019-7google scholar: lookup
  52. Irvine C, Alexander S. Factors affecting the circadian rhythm in plasma cortisol concentrations in the horse.. Domest. Anim. Endocrinol. 1994;11:227–238.
    doi: 10.1016/0739-7240(94)90030-2pubmed: 8045104google scholar: lookup
  53. Sousa M, Ziegler T. Diurnal variation on the excretion patterns of fecal steroids in common marmoset (Callithrix jacchus) females.. Am. J. Primatol. 1998;46:105–117.
    doi: 10.1002/(SICI)1098-2345(1998)46:2<105::AID-AJP1>3.0.CO;2-#pubmed: 9773674google scholar: lookup
  54. Chinnadurai S.K, Millspaugh J.J, Matthews W.S, Canter K, Slotow R, Washburn B.E, Woods R.J. Validation of fecal glucocorticoid metabolite assays for South African herbivores.. J. Wildl. Manag. 2009;73:1014–1020.
    doi: 10.2193/2008-430google scholar: lookup
  55. du Dot T.J, Rosen D.A, Richmond J.P, Kitaysky A.S, Zinn S.A, Trites A.W. Changes in glucocorticoids, IGF-I and thyroid hormones as indicators of nutritional stress and subsequent refeeding in Steller sea lions (Eumetopias jubatus). Comp. Biochem. Physiol. Part A Mol. Integr. Physiol. 2009;152:524–534.
    doi: 10.1016/j.cbpa.2008.12.010pubmed: 19146974google scholar: lookup
  56. Madosky J.M. Factors that Affect Harem Stability in a Feral Horse (Equus caballus) Population on Shackleford Banks Island, NC.. University of New Orleans; New Orleans, LA, USA: 2011.
  57. Pavitt A.T, Walling C.A, Möstl E, Pemberton J.M, Kruuk L.E. Cortisol but not testosterone is repeatable and varies with reproductive effort in wild red deer stags.. Gen. Comp. Endocrinol. 2015;222:62–68.
    doi: 10.1016/j.ygcen.2015.07.009pubmed: 26209865google scholar: lookup
  58. Borstel U.K.v, Visser E, Hall C. Indicators of stress in equitation.. Appl. Anim. Behav. Sci. 2017;190:43–56.
    doi: 10.1016/j.applanim.2017.02.018google scholar: lookup
  59. Liu R, Shi J, Liu D, Dong S, Zhang Y, Wu Y, Guo D. Effect of group size and reproductive status on faecal glucocorticoid concentration and vigilance in a free-ranging population of Przewalski’s gazelle.. Conserv. Physiol. 2020;8:coaa027.
    doi: 10.1093/conphys/coaa027pmc: PMC7125043pubmed: 32274069google scholar: lookup
  60. Voellmy I.K, Goncalves I.B, Barrette M.-F, Monfort S.L, Manser M.B. Mean fecal glucocorticoid metabolites are associated with vigilance, whereas immediate cortisol levels better reflect acute anti-predator responses in meerkats.. Horm. Behav. 2014;66:759–765.
    doi: 10.1016/j.yhbeh.2014.08.008pubmed: 25218254google scholar: lookup
  61. Bailey M.T, Coe C.L. Maternal separation disrupts the integrity of the intestinal microflora in infant rhesus monkeys.. Dev. Psychobiol. 1999;35:146–155.
    doi: 10.1002/(SICI)1098-2302(199909)35:2<146::AID-DEV7>3.0.CO;2-Gpubmed: 10461128google scholar: lookup
  62. Bonier F, Martin P.R, Moore I.T, Wingfield J.C. Do baseline glucocorticoids predict fitness?. Trends Ecol. Evol. 2009;24:634–642.
    doi: 10.1016/j.tree.2009.04.013pubmed: 19679371google scholar: lookup
  63. Ralph C, Tilbrook A. Invited review: The usefulness of measuring glucocorticoids for assessing animal welfare.. J. Anim. Sci. 2016;94:457–470.
    doi: 10.2527/jas.2015-9645pubmed: 27065116google scholar: lookup
  64. Lupien S.J, McEwen B.S, Gunnar M.R, Heim C. Effects of stress throughout the lifespan on the brain, behaviour and cognition.. Nat. Rev. Neurosci. 2009;10:434–445.
    doi: 10.1038/nrn2639pubmed: 19401723google scholar: lookup
  65. Tilbrook A.J, Turner A.I, Clarke I.J. Effects of stress on reproduction in non-rodent mammals: The role of glucocorticoids and sex differences.. Rev. Reprod. 2000;5:105–113.
    doi: 10.1530/ror.0.0050105pubmed: 10864855google scholar: lookup
  66. Pawluski J, Jego P, Henry S, Bruchet A, Palme R, Coste C, Hausberger M. Low plasma cortisol and fecal cortisol metabolite measures as indicators of compromised welfare in domestic horses (Equus caballus). PloS ONE 2017;12:e0182257.
    doi: 10.1371/journal.pone.0182257pmc: PMC5590897pubmed: 28886020google scholar: lookup
  67. Dawkins M.S. Behaviour as a tool in the assessment of animal welfare.. Zoology 2003;106:383–387.
    doi: 10.1078/0944-2006-00122pubmed: 16351922google scholar: lookup
  68. Mason G.J, Mendl M. Why is there no simple way of measuring animal welfare?. Anim. Welf. 1993;2:301–319.
  69. Young T, Creighton E, Smith T, Hosie C. A novel scale of behavioural indicators of stress for use with domestic horses.. Appl. Anim. Behav. Sci. 2012;140:33–43.
    doi: 10.1016/j.applanim.2012.05.008google scholar: lookup
  70. Heilmann T.J, Garrott R.A, Cadwell L.L, Tiller B.L. Behavioral response of free-ranging elk treated with an immunocontraceptive vaccine.. J. Wildl. Manag. 1998;62:243–250.
    doi: 10.2307/3802284google scholar: lookup
  71. McShea W.J, Monfort S.L, Hakim S, Kirkpatrick J, Liu I, Turner J.W, Chassy L, Munson L. The effect of immunocontraception on the behavior and reproduction of white-tailed deer.. J. Wildl. Manag. 1997;61:560–569.
    doi: 10.2307/3802615google scholar: lookup

Citations

This article has been cited 5 times.
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  5. Górecka-Bruzda A, Jaworska J, Stanley CR. The Social and Reproductive Challenges Faced by Free-Roaming Horse (Equus caballus) Stallions. Animals (Basel) 2023 Mar 24;13(7).
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