Larvae and adult flies of Rhinoestrus purpureus and R. usbekistanicus: morphology and pupation (Diptera: Oestridae).

The research study delves into the physical attributes and development phases of two specific species of Rhinoestrus, namely R. purpureus and R. usbekistanicus. The team used light and scanning electron microscopy to examine these species’ larvae and adult flies, revealing noticeable differences in detailed characteristics.

Objective and Methodology

• The study’s main aim was to descriptively identify and compare several aspects of the two Rhinoestrus species. This includes their larvae, adult flies, and pupation times.
• The research team used light and scanning electron microscopy to achieve their objectives. These technologies were able to present a clear and detailed observation of the characteristics and differences of the two Rhinoestrus species.

Findings on Larvae Characteristics

• Observable differences were found between the R. purpureus and R. usbekistanicus larvae, particularly:
  • Spination pattern, or the pattern of sharp point-like projections.
  • The shape of the spines on the larvae.
  • The shape and ultrastructures of the peritremes, structures involved in respiration.

Findings on Pupation Period

• For both the R. purpureus and R. usbekistanicus species, the pupa stage was found to last between 15 to 21 days at room temperature.
• Following this period, the larvae develop into adult flies.

Adult Flies Morphology

• The external genitalia of both male and female adult flies were identified.
• Physical features of R. purpureus and R. usbekistanicus adult flies were distinguished, including:
  • The disposition of parafrontalia and parafascialia tubercles, which are bumps or projections on the flies’ bodies.
  • Mesonotal weals, which refer to the welts or swollen areas on the cuticles of the flies.
  • Wings and abdominal pellonisity, or the hairy or bristly coverings on the abdomen of the flies.
• In addition, the antennae of the adult flies were examined, and the sensillae (sensory organs) were compared between the two species.