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Home / Equine Research Bank / Eur Cell Mater / Lentiviral-based reporter constructs for profiling chondroge...
European cells & materials2018; 36; 156-170; doi: 10.22203/eCM.v036a12

Lentiviral-based reporter constructs for profiling chondrogenic activity in primary equine cell populations.

Abstract: Successful clinical translation of mesenchymal stem cell (MSC)-based therapies for cartilage repair will likely require the implementation of standardised protocols and broadly applicable tools to facilitate the comparisons among cell types and chondroinduction methods. The present study investigated the utility of recombinant lentiviral reporter vectors as reliable tools for comparing chondrogenic potential among primary cell populations and distinguishing cellular-level variations of chondrogenic activity in widely used three-dimensional (3D) culture systems. Primary equine MSCs and chondrocytes were transduced with vectors containing combinations of fluorescent and luciferase reporter genes under constitutive cytomeglavirus (CMV) or chondrocyte-lineage (Col2) promoters. Reporter activity was measured by fluorescence imaging and luciferase assay. In 3D cultures of MSC aggregates and polyethylene glycol-hyaluronic acid (PEG-HA) hydrogels, transforming growth factor beta 3 (TGF-β3)-mediated chondroinduction increased Col2 reporter activity, demonstrating close correlation with histology and mRNA expression levels of COL2A1 and SOX9. Comparison of chondrogenic activities among MSC populations using a secretable luciferase reporter revealed enhanced chondrogenesis in bone-marrow-derived MSCs relative to MSC populations from synovium and adipose tissues. A dual fluorescence reporter - enabling discrimination of highly chondrogenic (Col2-GFP) cells within an MSC population (CMV-tdTomato) - revealed marked heterogeneity in differentiating aggregate cultures and identified chondrogenic cells in chondrocyte-seeded PEG-HA hydrogels after 6 weeks in a subcutaneous implant model - indicating stable, long-term reporter expression in vivo. These results suggested that lentiviral reporter vectors may be used to address fundamental questions regarding chondrogenic activity in chondroprogenitor cell populations and accelerate clinical translation of cell-based cartilage repair strategies.
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Publication Date: 2018-10-12 PubMed ID: 30311630PubMed Central: PMC6788286DOI: 10.22203/eCM.v036a12Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the utility of lentiviral reporter vectors as tools for comparing the potential to develop into cartilage among different primary cell populations. The study used equine MSCs and chondrocytes and found that these vectors can be used effectively in common three-dimensional cell culture systems. The study also brought light to differences in the chondrogenic activity among different types of MSCs.

Objective of the Research

  • The objective of the research was to test the utility of recombinant lentiviral reporter vectors as tools for comparing the chondrogenic activity among various primary cell populations. The study further aimed to investigate variability in chondrogenic activity in widely used three-dimensional (3D) culture systems.

Methodology and Findings

  • The researchers used primary equine mesenchymal stem cells (MSCs) and chondrocytes, which were transduced with vectors containing a combination of fluorescent and luciferase reporter genes under constitutive cytomegalovirus (CMV) or chondrocyte-lineage (Col2) promoters.
  • They then measured the reporter activity using fluorescence imaging and luciferase assays.
  • Furthermore, they discovered that in 3D cultures of MSC aggregates and polyethylene glycol-hyaluronic acid (PEG-HA) hydrogels, transforming growth factor beta 3 (TGF-β3) increased Col2 reporter activity, showing a close correlation with histology and mRNA expression levels of COL2A1 and SOX9.
  • The research revealed enhanced chondrogenesis in bone-marrow-derived MSCs compared to MSC populations from synovium and adipose tissues when using a secretable luciferase reporter.

Significant Conclusions

  • A crucial finding was that a dual fluorescence reporter could distinguish highly chondrogenic cells (Col2-GFP) within an MSC population. This indicative of marked heterogeneity in differentiating aggregate cultures was identified.
  • Moreover, the study revealed that this system could detect chondrogenic cells in chondrocyte-seeded PEG-HA hydrogels after six weeks in a subcutaneous implant model, indicating stable, long-term reporter expression in vivo.
  • Collectively, the findings suggested that lentiviral reporter vectors might be a helpful tool to explore fundamental questions regarding chondrogenic activity in chondroprogenitor cell populations and could potentially accelerate the clinical translation of cell-based cartilage repair strategies.

Cite This Article

APA
Martin-Pena A, Porter RM, Plumton G, McCarrel TM, Morton AJ, Guijarro MV, Ghivizzani SC, Sharma B, Palmer GD. (2018). Lentiviral-based reporter constructs for profiling chondrogenic activity in primary equine cell populations. Eur Cell Mater, 36, 156-170. https://doi.org/10.22203/eCM.v036a12

Publication

European cells & materials
ISSN: 1473-2262
NlmUniqueID: 100973416
Country: Switzerland
Language: English
Volume: 36
Pages: 156-170

Researcher Affiliations

Martin-Pena, A
    Porter, R M
      Plumton, G
        McCarrel, T M
          Morton, A J
            Guijarro, M V
              Ghivizzani, S C
                Sharma, B
                  Palmer, G D
                  • Department of Orthopaedics and Rehabilitation, University of Florida, 1600 SW Archer Rd, MSB, M2-235, Gainesville, FL, 32610, USA.glynpal@gmail.com.

                  MeSH Terms

                  • Animals
                  • Cell Aggregation
                  • Cell Differentiation
                  • Cells, Cultured
                  • Chondrocytes / cytology
                  • Chondrocytes / metabolism
                  • Chondrogenesis
                  • Collagen Type II / genetics
                  • Collagen Type II / metabolism
                  • Fluorescence
                  • Genes, Reporter
                  • Horses
                  • Hyaluronic Acid / pharmacology
                  • Hydrogen / pharmacology
                  • Implants, Experimental
                  • Lentivirus / genetics
                  • Luciferases / metabolism
                  • Mesenchymal Stem Cells / cytology
                  • Mesenchymal Stem Cells / metabolism
                  • Polyethylene Glycols / pharmacology
                  • Promoter Regions, Genetic / genetics

                  Grant Funding

                  • R21 AR069844 / NIAMS NIH HHS

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                  Citations

                  This article has been cited 2 times.
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