Liquid chromatography-tandem mass spectrometric method for determination of mosapride citrate in equine tissues.

The abstract describes a newly developed method for determining the levels of mosapride citrate and its metabolite in equine tissues. The method involves using liquid chromatography-tandem mass spectrometry, displaying a wide linear range for both substances and a high degree of precision.

Methodology

• The researchers used a simple method to detect levels of mosapride citrate and its des-p-fluorobenzyl mosapride (M-1) metabolite in a variety of equine tissues, including muscle, liver, kidney, adipose tissue, and intestine.
• (+/-)-4-Amino-5-chloro-2-ethoxy-N-[[4-(2-chlorobenzyl)morpholinyl]methyl]benzamide was used as an internal standard.
• The test substances were spiked and extracted from the tissues using acetonitrile.
• The researchers used a TSK-GEL SUPER ODS column to perform the chromatographic separation. The mobile phase consisted of acetonitrile-0.05% (v/v) formic acid containing 5 mmol/L nonafluoropentanoic acid (2:3, v/v).

Results

• The method displayed a large linear range from 0.0005 to 0.2 microg/mL for both the mosapride citrate and M-1 (r>0.9976).
• During the intra-day assay (n=5), the relative standard deviations (RSDs) were between 1.1 to 7.8% for mosapride citrate and 1.6 to 7.2% for M-1.
• During the inter-day assay (n=3), the RSDs were between 1.0 to 13% for mosapride citrate and 0.8 to 11% for M-1.
• The extraction recovery at 1.28 microg/g of mosapride citrate from equine tissues ranged between 97 to 107%.
• The lower limit of quantification for mosapride citrate was determined to be 0.004 microg/g.
• Stability studies were also conducted at different storage conditions.

Conclusion

• The described method demonstrated reliable, precise, and accurate findings.
• The method could potentially be utilized to determine the levels of mosapride citrate and its metabolite in tissue samples.