Localization and secretion of inhibins in the equine fetal ovaries.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research article investigates the source of inhibins in horse fetuses, discovering that these chemicals are produced by interstitial cells in the fetal ovary rather than being produced maternally. This finding could have important implications for understanding how the development of reproductive organs in equine fetuses is regulated.
Research Objective and Methods
The study was conducted to identify the source of inhibins in equine fetuses. The methodological approach used to achieve this objective involved measuring concentrations of various forms of inhibin in both fetal and maternal circulation systems and fetal ovaries. The research also examined the location of different inhibin subunits and expression of associated mRNA using immunohistochemistry and in situ hybridization.
- Immunoreactive inhibin, inhibin pro-alphaC, and inhibin A were the specific forms of inhibin targeted in these investigations.
- The focus was on fetal and maternal samples collected between 100 and 250 days of gestation, a critical developmental period.
Key Findings
The research found that levels of immunoreactive inhibin, inhibin pro-alphaC, and inhibin A were significantly higher in the fetal circulation as opposed to the maternal circulation during the specified gestational period.
- The fetal ovaries contained high amounts of the investigated forms of inhibin.
- Contrary to this, these inhibin forms were not detectable in either the maternal ovaries or the placenta.
Protein Localization and mRNA Expression
The study further revealed that inhibin alpha and inhibin/activin beta(A) and beta(B) subunit proteins were found in the enlarged interstitial cells of the equine fetal ovary.
- The expression of inhibin alpha and inhibin/activin beta(A) subunit mRNAs paralleled this protein localization, also observed in the interstitial cells.
Conclusion and Implication
From these findings, the researchers concluded that the main source of inhibins in fetal circulation is the interstitial cells of the fetal ovary, not of maternal origin. This has implications for understanding the physiological roles inhibins play in the development of fetal gonads, potentially influencing future reproductive health studies in equines.
Cite This Article
Publication
Researcher Affiliations
- Department of Basic Veterinary Science, The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.
MeSH Terms
- Animals
- Female
- Fetus / metabolism
- Horses / anatomy & histology
- Horses / physiology
- Inhibin-beta Subunits / genetics
- Inhibin-beta Subunits / metabolism
- Inhibins / genetics
- Inhibins / metabolism
- Maternal-Fetal Exchange
- Organ Size
- Ovary / anatomy & histology
- Ovary / metabolism
- Pregnancy
- RNA, Messenger / genetics
- RNA, Messenger / metabolism
Citations
This article has been cited 4 times.- Dhakal P, Tsunoda N, Nambo Y, Taniyama H, Nagaoka K, Watanabe G, Taya K. Circulating activin A during equine gestation and immunolocalization of its receptors system in utero-placental tissues and fetal gonads. J Equine Sci 2021 Jun;32(2):39-48.
- Araki M, Ohtaki T, Kimura J, Hobo S, Taya K, Tsunoda N, Taniyama H, Tsumagari S, Nambo Y. Presence of surfactant proteins in the uteri and placentae of pregnant mares. J Vet Med Sci 2021 Jul 28;83(7):1167-1172.
- Dhakal P, Tsunoda N, Nakai R, Nagaoka K, Nambo Y, Sato F, Taniyama H, Taya K. Post-Natal Dynamic Changes in Circulating Follicle-Stimulating Hormone, Luteinizing Hormone, Immunoreactive Inhibin, Progesterone, Testosterone and Estradiol-17β in Thoroughbred Colts until 6 Months of Age. J Equine Sci 2011;22(1):9-15.
- Yang P, Medan MS, Arai KY, Jin W, Watanabe G, Taya K. Secretion of inhibin and testicular expression of inhibin subunits in male duck embryos and newly hatched ducks. Endocrine 2005 Nov;28(2):171-9.
