Low-density lipoproteins and milk serum proteins improve the quality of stallion sperm after vitrification in straws.
Abstract: Lipids and proteins can be used for sperm vitrification to preserve the integrity of sperm membranes or to increase the viscosity of the medium. This study evaluated the effect of low-density lipoproteins (LDL) and milk serum proteins (Pronexcell) for stallion sperm vitrification. Hippex extender (Barex Biochemical Products, The Netherlands), plus 1% of bovine serum albumin and 100 mM of trehalose, was used as control for sperm vitrification. In experiment 1, different concentrations of LDL (L1 = 0.25, L2 = 0.5, L3 = 1%) and in experiment 2 of Pronexcell (P1 = 1, P2 = 5, P3 = 10%) were added to control extender. Vitrification was performed in 0.25-ml straws directly plunged into liquid nitrogen. Total motility (TM, %) and progressive motility (PM, %) were analysed by CASA, and plasma membrane (IMS, %) and acrosome membrane integrity (AIS, %) were assessed under epifluorescence microscopy. Post-warmed sperm parameters were compared between treatments by ANOVA. Results were expressed as mean ± SEM. In both experiments, the minimum concentration of LDL and Pronexcell obtained significantly higher values (p < 0.01) than the control extender for TM (L1 = 52.95 ± 4.4; P1 = 58.99 ± 4.6; C = 30.88 ± 3.0), PM (L1 = 36.79 ± 5.5; P1 = 47.25 ± 4.3; C = 19.20 ± 2.4), IMS (L1 = 68.88 ± 3.6; P1 = 47.25 ± 4.3; C = 52.81 ± 2.6) and AIS (L1 = 45.88 ± 3.6; P1 = 47.25 ± 4.3; C = 26.00 ± 2.1). No differences in sperm parameters were found among different concentrations of LDL or Pronexcell. In conclusion, the addition of 0.25% LDL and 1% Pronexcell to the vitrification extender is recommended to improve the quality of stallion sperm after vitrification.
© 2019 Blackwell Verlag GmbH.
Publication Date: 2019-10-19 PubMed ID: 31625235DOI: 10.1111/rda.13495Google Scholar: Lookup
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- Journal Article
Summary
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The research article investigates how low-density lipoproteins (LDL) and milk serum proteins (Pronexcell) can be used to improve the quality of horse sperm after a preservation process known as vitrification. The study found that both LDL and Pronexcell significantly improved sperm motility and integrity after preservation.
Research Methodology
- The researchers conducted two experiments. They used a preservation medium, Hippex extender, supplemented with bovine serum albumin and trehalose as a control for sperm vitrification.
- In the first experiment, different concentrations of LDL were added to the control extender. The concentrations were 0.25% (L1), 0.5% (L2), and 1% (L3).
- In the second experiment, different concentrations of the milk serum protein Pronexcell were added to the control extender. The concentrations were 1% (P1), 5% (P2), and 10% (P3).
- Vitrification was performed using 0.25-ml straws, which were then directly plunged into liquid nitrogen.
Data Collection and Analysis
- Sperm parameters including total motility (TM), progressive motility (PM), plasma membrane integrity (IMS), and acrosome membrane integrity (AIS) were evaluated.
- The parameters were analyzed using Computer-assisted Sperm Analysis (CASA) and examined under epifluorescence microscopy.
- The post-warmed sperm parameters were compared between treatments by ANOVA (Analysis of Variance) and results were expressed as mean ± SEM (Standard Error of Mean).
Findings
- The results showed that both LDL and Pronexcell significantly improved total motility, progressive motility, and plasma and acrosome membrane integrity compared to the control extender.
- No significant differences in sperm parameters were found among different concentrations of LDL or Pronexcell.
Conclusion
- In conclusion, the addition of 0.25% LDL and 1% Pronexcell to the vitrification extender is recommended to improve the quality of stallion sperm after vitrification.
- These findings suggest that lipids (like LDL) and proteins (like Pronexcell) are useful in the preservation of sperm, potentially leading to more successful breeding outcomes in the long term.
Cite This Article
APA
Consuegra C, Crespo F, Dorado J, Diaz-Jimenez M, Pereira B, Hidalgo M.
(2019).
Low-density lipoproteins and milk serum proteins improve the quality of stallion sperm after vitrification in straws.
Reprod Domest Anim, 54 Suppl 4, 86-89.
https://doi.org/10.1111/rda.13495 Publication
Researcher Affiliations
- Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Centro Militar de Cría Caballar, Avila, Spain.
- Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
- Veterinary Reproduction Group, University of Cordoba, Cordoba, Spain.
MeSH Terms
- Acrosome / drug effects
- Animals
- Cryopreservation / methods
- Cryopreservation / veterinary
- Cryoprotective Agents / pharmacology
- Horses
- Lipoproteins, LDL / pharmacology
- Male
- Milk Proteins / pharmacology
- Semen Analysis / veterinary
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility / drug effects
- Spermatozoa / cytology
- Spermatozoa / drug effects
- Vitrification
References
This article includes 14 references
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Citations
This article has been cited 2 times.- Diaz-Jimenez M, Wang M, Wang W, Isachenko E, Rahimi G, Kumar P, Mallmann P, von Brandenstein M, Hidalgo M, Isachenko V. Cryo-banking of human spermatozoa by aseptic cryoprotectants-free vitrification in liquid air: Positive effect of elevated warming temperature. Cell Tissue Bank 2022 Mar;23(1):17-29.
- Hidalgo M, Ortiz I. Sperm Vitrification in Horse and Donkey. Methods Mol Biol 2025;2897:137-145.
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