Maintenance of equine articular cartilage explants in serum-free and serum-supplemented media, compared with that in a commercial supplemented medium.
Abstract: To evaluate the effects of a commercially defined, serum-free medium additive on equine articular cartilage explants, compared with effects of serum-free and serum-supplemented media. Methods: Articular cartilage from a 3-year-old, mixed breed horse euthanatized for reasons other than musculoskeletal disease or sepsis. Methods: Media were changed every 48 hours, and the glycosaminoglycan (GAG) content was determined in media collected at each time point. Glycosaminoglycan synthesis by explant chondrocytes, and residual GAG content of articular cartilage (as a measure of explant GAG loss) were determined at the end of the study (day 8). Results: Articular cartilage explants in serum-free medium and the commercial supplemented medium had significantly lower GAG synthesis and GAG content than did those incubated in serum-supplemented medium. There were no significant differences in GAG synthesis and content between serum-free and commercial supplemented medium groups. When comparing medium GAG content for all treatment groups, the GAG content in serum-free medium on day 8 was significantly greater than that in commercial supplemented medium, but significant differences were not evident in percentage of release of GAG (as an indicator of GAG degradation) among all 3 treatment groups. Conclusions: Commercial supplemented medium had effects on articular cartilage matrix GAG loss into medium equal to those of serum-supplemented medium (eg, both lost articular cartilage explant GAG to a similar degree). However, residual articular cartilage GAG content was higher in serum-supplemented medium, as was GAG synthesis. Commercial supplemented medium appears to either lack the proper ingredients to maintain steady-state GAG synthesis, or lacks proper concentrations of these ingredients.
Publication Date: 1996-09-01 PubMed ID: 8874717
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Articular Cartilage
- Biochemistry
- Cartilage
- Cell Culture
- Clinical Study
- Comparative Study
- Equine Health
- Equine Science
- Experimental Methods
- Extracellular matrix
- Glycosaminoglycan
- In Vitro Research
- Laboratory Methods
- Metabolism
- Musculoskeletal System
- Physiology
- Serum
- Veterinary Care
- Veterinary Medicine
- Veterinary Research
Summary
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The researchers in this study sought to understand the impact of a commercially available, serum-free medium supplement on equine articular cartilage tissue samples, comparing it to serum-free and serum-supplemented media. It was found that both the serum-free medium and the supplemented medium resulted in significantly lower synthesis and retention of glycosaminoglycan (GAG) compared to the serum-supplemented medium.
Research Methods
- The researchers used articular cartilage samples from a 3-year-old, mixed breed horse that was euthanized for reasons unrelated to musculoskeletal diseases or sepsis.
- The media in which the cartilage samples were maintained were changed every 48 hours.
- The glycosaminoglycan (GAG) content was checked in the collected media at each interval.
- At the end of the study on the 8th day, GAG synthesis by the tissue samples and residual GAG content of the cartilage (a measure for tissue GAG loss) were determined.
Findings
- The study found that articular cartilage samples maintained in serum-free and commercial supplemented media had a significantly lower GAG synthesis and content compared to the samples in serum-supplemented medium.
- Neither the serum-free nor the commercial supplemented medium groups showed significant differences with respect to GAG synthesis and content.
- On comparing the GAG content in the media for all the treatment groups, the content in the serum-free medium on day 8 was significantly higher than in the commercially supplemented medium.
- However, there were no significant differences observed in the release percentage of GAG (an indicator of GAG degradation) across all three treatment groups.
Conclusions
- The effects of the commercial supplemented medium on articular cartilage matrix GAG loss into the medium were the same as those of the serum-supplemented medium. Both mediums resulted in a similar degree of loss of GAG from the tested articular cartilage samples.
- However, the residual GAG content of the articular cartilage was higher in serum-supplemented medium, as was the GAG synthesis.
- The study concluded that the commercial supplemented medium either lacks the necessary ingredients to maintain stable GAG synthesis, or has unsuitable concentrations of these ingredients
Cite This Article
APA
Kawcak CE, Trotter GW, Frisbie DD, McIlwraith CW.
(1996).
Maintenance of equine articular cartilage explants in serum-free and serum-supplemented media, compared with that in a commercial supplemented medium.
Am J Vet Res, 57(9), 1261-1265.
Publication
Researcher Affiliations
- Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, Fort Collins 80523, USA.
MeSH Terms
- Analysis of Variance
- Animals
- Cartilage, Articular / cytology
- Cartilage, Articular / physiology
- Culture Media
- Culture Media, Serum-Free
- Glycosaminoglycans / analysis
- Glycosaminoglycans / biosynthesis
- Glycosaminoglycans / metabolism
- Horses
- Kinetics
- Organ Culture Techniques / methods
Citations
This article has been cited 2 times.- Garbin LC, McIlwraith CW, Frisbie DD. Use of allogeneic freeze-dried conditioned serum for the prevention of degradation in cartilage exposed to IL-1ß. BMC Vet Res 2022 Jul 11;18(1):265.
- Camargo Garbin L, McIlwraith CW, Frisbie DD. Evaluation of allogeneic freeze-dried platelet lysate in cartilage exposed to interleukin 1-β in vitro. BMC Vet Res 2019 Nov 1;15(1):386.
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