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Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2001; 9(1); 53-59; doi: 10.1023/a:1026743700819

Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis.

Abstract: We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libraries were screened with PCR for clones containing these 13 Type I loci, nine of which were found in the libraries. BAC clones were used as probes in dual colour FISH to confirm their precise chromosomal origin. The remaining four genes were mapped in a somatic cell hybrid panel. All chromosomal assignments except one were in agreement with human-horse ZOO-FISH data and revealed new and more detailed information on the equine comparative map. CLU was mapped by synteny to ECA2 while human-horse ZOO-FISH data predicted that CLU would be located on ECA9. The assignment of IL1RN permitted analysis of gene order conservation between HSA2 and ECA15, which identified that an event of inversion had occurred during the evolution of these two homologous chromosomes.
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Publication Date: 2001-03-29 PubMed ID: 11272792DOI: 10.1023/a:1026743700819Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the application of two gene mapping techniques – fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis for the study of 13 different horse genes. Notably, the research also uncovers some previously unknown information about the equine comparative map.

Overview of the Methods

  • Details on how specific primers, designed from horse DNA or mRNA sequences found in GenBank, were used to trigger the PCR amplification of untranslated or intronic gene regions.
  • The researchers used two distinct bacterial artificial chromosome (BAC) libraries of the horse, which were assessed with PCR to identify clones containing any of the 13 Type I loci under investigation.
  • The use of BAC clones as probes in dual colour FISH for determining their accurate chromosomal origin.
  • The mapping of four genes using a somatic cell hybrid panel is noted. This technique utilizes hybrid cells created from two distinct organisms to study and map genes on chromosomes.

Findings

  • Among the 13 genes studied, nine were identified within the two horse BAC libraries.
  • All the chromosomal assignments of these genes, except for one, were consistent with the existing human-horse ZOO-FISH data.
  • However, the CLU gene was found, through synteny mapping, on chromosome ECA2, while the previous human-horse ZOO-FISH data had placed it on ECA9.
  • The positioning of the IL1RN gene was instrumental in analysing the conservation of gene order between HSA2 (a human chromosome) and ECA15 (a horse chromosome), highlighting an event of inversion in the evolutionary history of these two homologous chromosomes.

Significance

The research contributed significant new insights and details to the equine comparative map. It shed light on the feasibility of applying FISH and somatic cell hybrid analyses in studying horse genes and demonstrates the potential for unearthing new information about horse genetics through these methods.

Cite This Article

APA
Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. (2001). Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis. Chromosome Res, 9(1), 53-59. https://doi.org/10.1023/a:1026743700819

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 9
Issue: 1
Pages: 53-59

Researcher Affiliations

Lindgren, G
  • Department of Evolutionary Biology, Norbyvägen, Uppsala University, Sweden. gabriella.lindgren@ebc.uu.se
Breen, M
    Godard, S
      Bowling, A
        Murray, J
          Scavone, M
            Skow, L
              Sandberg, K
                Guérin, G
                  Binns, M
                    Ellegren, H

                      MeSH Terms

                      • Animals
                      • Chromosome Mapping
                      • Gene Library
                      • Horses / genetics
                      • Hybrid Cells
                      • In Situ Hybridization, Fluorescence
                      • Metaphase
                      • Polymerase Chain Reaction

                      References

                      This article includes 25 references
                      1. Swinburne J, Gerstenberg C, Breen M, Aldridge V, Lockhart L, Marti E, Antczak D, Eggleston-Stott M, Bailey E, Mickelson J, Røed K, Lindgren G, von Haeringen W, Guérin G, Bjarnason J, Allen T, Binns M. First comprehensive low-density horse linkage map based on two 3-generation, full-sibling, cross-bred horse reference families.. Genomics 2000 Jun 1;66(2):123-34.
                        pubmed: 10860657doi: 10.1006/geno.2000.6207google scholar: lookup
                      2. Shiue Y-L, Millon LV, Skow LC, Honeycutt D, Murray JD, Bowling AT. Synteny and regional marker order assignment of 26 type I and microsatellite markers to the horse X- and Y-chromosomes.. Chromosome Res 2000;8(1):45-55.
                        pubmed: 10730588doi: 10.1023/a:1009275102977google scholar: lookup
                      3. Bailey E, Graves KT, Cothran EG, Reid R, Lear TL, Ennis RB. Synteny-mapping horse microsatellite markers using a heterohybridoma panel.. Anim Genet 1995 Jun;26(3):177-80.
                        pubmed: 7793685doi: 10.1111/j.1365-2052.1995.tb03158.xgoogle scholar: lookup
                      4. de Gortari MJ, Freking BA, Cuthbertson RP, Kappes SM, Keele JW, Stone RT, Leymaster KA, Dodds KG, Crawford AM, Beattie CW. A second-generation linkage map of the sheep genome.. Mamm Genome 1998 Mar;9(3):204-9.
                        pubmed: 9501303doi: 10.1007/s003359900726google scholar: lookup
                      5. Raudsepp T, Frönicke L, Scherthan H, Gustavsson I, Chowdhary BP. Zoo-FISH delineates conserved chromosomal segments in horse and man.. Chromosome Res 1996 Apr;4(3):218-25.
                        pubmed: 8793207doi: 10.1007/BF02254963google scholar: lookup
                      6. Chevalet C, Corpet F. Statistical decision rules concerning synteny or independence between markers.. Cytogenet Cell Genet 1986;43(3-4):132-9.
                        pubmed: 3467898doi: 10.1159/000132311google scholar: lookup
                      7. Kappes SM, Keele JW, Stone RT, McGraw RA, Sonstegard TS, Smith TP, Lopez-Corrales NL, Beattie CW. A second-generation linkage map of the bovine genome.. Genome Res 1997 Mar;7(3):235-49.
                        pubmed: 9074927doi: 10.1101/gr.7.3.235google scholar: lookup
                      8. Breen M, Langford CF, Carter NP, Holmes NG, Dickens HF, Thomas R, Suter N, Ryder EJ, Pope M, Binns MM. FISH mapping and identification of canine chromosomes.. J Hered 1999 Jan-Feb;90(1):27-30.
                        pubmed: 9987898doi: 10.1093/jhered/90.1.27google scholar: lookup
                      9. Hasler-Rapacz J, Ellegren H, Fridolfsson AK, Kirkpatrick B, Kirk S, Andersson L, Rapacz J. Identification of a mutation in the low density lipoprotein receptor gene associated with recessive familial hypercholesterolemia in swine.. Am J Med Genet 1998 Apr 13;76(5):379-86.
                        pubmed: 9556295
                      10. Guérin G, Bailey E, Bernoco D, Anderson I, Antczak DF, Bell K, Binns MM, Bowling AT, Brandon R, Cholewinski G, Cothran EG, Ellegren H, Förster M, Godard S, Horin P, Ketchum M, Lindgren G, McPartlan H, Mériaux JC, Mickelson JR, Millon LV, Murray J, Neau A, Røed K, Ziegle J. Report of the International Equine Gene Mapping Workshop: male linkage map.. Anim Genet 1999 Oct;30(5):341-54.
                        pubmed: 10582279doi: 10.1046/j.1365-2052.1999.00510.xgoogle scholar: lookup
                      11. Johansson M, Ellegren H, Andersson L. Comparative mapping reveals extensive linkage conservation--but with gene order rearrangements--between the pig and the human genomes.. Genomics 1995 Feb 10;25(3):682-90.
                        pubmed: 7759103doi: 10.1016/0888-7543(95)80011-agoogle scholar: lookup
                      12. Caetano AR, Pomp D, Murray JD, Bowling AT. Comparative mapping of 18 equine type I genes assigned by somatic cell hybrid analysis.. Mamm Genome 1999 Mar;10(3):271-6.
                        pubmed: 10051323doi: 10.1007/s003359900985google scholar: lookup
                      13. Shiue YL, Bickel LA, Caetano AR, Millon LV, Clark RS, Eggleston ML, Michelmore R, Bailey E, Guérin G, Godard S, Mickelson JR, Valberg SJ, Murray JD, Bowling AT. A synteny map of the horse genome comprised of 240 microsatellite and RAPD markers.. Anim Genet 1999 Feb;30(1):1-9.
                        pubmed: 10050277doi: 10.1046/j.1365-2052.1999.00377.xgoogle scholar: lookup
                      14. Werner P, Mellersh CS, Raducha MG, DeRose S, Acland GM, Prociuk U, Wiegand N, Aguirre GD, Henthorn PS, Patterson DF, Ostrander EA. Anchoring of canine linkage groups with chromosome-specific markers.. Mamm Genome 1999 Aug;10(8):814-23.
                        pubmed: 10430668doi: 10.1007/s003359901096google scholar: lookup
                      15. Bailey E, Reid RC, Skow LC, Mathiason K, Lear TL, McGuire TC. Linkage of the gene for equine combined immunodeficiency disease to microsatellite markers HTG8 and HTG4; synteny and FISH mapping to ECA9.. Anim Genet 1997 Aug;28(4):268-73.
                        pubmed: 9345723doi: 10.1111/j.1365-2052.1997.00152.xgoogle scholar: lookup
                      16. Menotti-Raymond M, David VA, Lyons LA, Schäffer AA, Tomlin JF, Hutton MK, O'Brien SJ. A genetic linkage map of microsatellites in the domestic cat (Felis catus).. Genomics 1999 Apr 1;57(1):9-23.
                        pubmed: 10191079doi: 10.1006/geno.1999.5743google scholar: lookup
                      17. O'Brien SJ, Womack JE, Lyons LA, Moore KJ, Jenkins NA, Copeland NG. Anchored reference loci for comparative genome mapping in mammals.. Nat Genet 1993 Feb;3(2):103-12.
                        pubmed: 8499943doi: 10.1038/ng0293-103google scholar: lookup
                      18. Breen M, Lindgren G, Binns MM, Norman J, Irvin Z, Bell K, Sandberg K, Ellegren H. Genetical and physical assignments of equine microsatellites--first integration of anchored markers in horse genome mapping.. Mamm Genome 1997 Apr;8(4):267-73.
                        pubmed: 9096108doi: 10.1007/s003359900407google scholar: lookup
                      19. Bowling AT, Breen M, Chowdhary BP, Hirota K, Lear T, Millon LV, Ponce de Leon FA, Raudsepp T, Stranzinger G. International system for cytogenetic nomenclature of the domestic horse. Report of the Third International Committee for the Standardization of the domestic horse karyotype, Davis, CA, USA, 1996.. Chromosome Res 1997 Nov;5(7):433-43.
                        pubmed: 9421259doi: 10.1023/a:1018408811881google scholar: lookup
                      20. Lindgren G, Sandberg K, Persson H, Marklund S, Breen M, Sandgren B, Carlstén J, Ellegren H. A primary male autosomal linkage map of the horse genome.. Genome Res 1998 Sep;8(9):951-66.
                        pubmed: 9750194doi: 10.1101/gr.8.9.951google scholar: lookup
                      21. Oakenfull EA, Buckle VJ, Clegg JB. Localization of the horse (Equus caballus) alpha-globin gene complex to chromosome 13 by fluorescence in situ hybridization.. Cytogenet Cell Genet 1993;62(2-3):136-8.
                        pubmed: 8428512doi: 10.1159/000133456google scholar: lookup
                      22. Caetano AR, Shiue YL, Lyons LA, O'Brien SJ, Laughlin TF, Bowling AT, Murray JD. A comparative gene map of the horse (Equus caballus).. Genome Res 1999 Dec;9(12):1239-49.
                        pubmed: 10613847doi: 10.1101/gr.9.12.1239google scholar: lookup
                      23. Williams H, Richards CM, Konfortov BA, Miller JR, Tucker EM. Synteny mapping in the horse using horse-mouse heterohybridomas.. Anim Genet 1993 Aug;24(4):257-60.
                        pubmed: 8239069doi: 10.1111/j.1365-2052.1993.tb00308.xgoogle scholar: lookup
                      24. Godard S, Schibler L, Oustry A, Cribiu EP, Guérin G. Construction of a horse BAC library and cytogenetical assignment of 20 type I and type II markers.. Mamm Genome 1998 Aug;9(8):633-7.
                        pubmed: 9680383doi: 10.1007/s003359900835google scholar: lookup
                      25. Godard S, Vaiman D, Oustry A, Nocart M, Bertaud M, Guzylack S, Mériaux JC, Cribiu EP, Guérin G. Characterization, genetic and physical mapping analysis of 36 horse plasmid and cosmid-derived microsatellites.. Mamm Genome 1997 Oct;8(10):745-50.
                        pubmed: 9321468doi: 10.1007/s003359900558google scholar: lookup

                      Citations

                      This article has been cited 1 times.
                      1. Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
                        doi: 10.1007/s10577-008-1204-zpubmed: 18274866google scholar: lookup
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