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Home / Equine Research Bank / Chromosome Res / Mapping of 13 horse genes by fluorescence in-situ hybridizat...
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology2001; 9(1); 53-59; doi: 10.1023/a:1026743700819

Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis.

Abstract: We report fluorescence in-situ hybridization (FISH) and somatic cell hybrid mapping data for 13 different horse genes (ANP, CD2, CLU, CRISP3, CYP17, FGG, IL1RN, IL10, MMP13, PRM1, PTGS2, TNFA and TP53). Primers for PCR amplification of intronic or untranslated regions were designed from horse-specific DNA or mRNA sequences in GenBank. Two different horse bacterial artificial chromosome (BAC) libraries were screened with PCR for clones containing these 13 Type I loci, nine of which were found in the libraries. BAC clones were used as probes in dual colour FISH to confirm their precise chromosomal origin. The remaining four genes were mapped in a somatic cell hybrid panel. All chromosomal assignments except one were in agreement with human-horse ZOO-FISH data and revealed new and more detailed information on the equine comparative map. CLU was mapped by synteny to ECA2 while human-horse ZOO-FISH data predicted that CLU would be located on ECA9. The assignment of IL1RN permitted analysis of gene order conservation between HSA2 and ECA15, which identified that an event of inversion had occurred during the evolution of these two homologous chromosomes.
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Publication Date: 2001-03-29 PubMed ID: 11272792DOI: 10.1023/a:1026743700819Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article discusses the application of two gene mapping techniques – fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis for the study of 13 different horse genes. Notably, the research also uncovers some previously unknown information about the equine comparative map.

Overview of the Methods

  • Details on how specific primers, designed from horse DNA or mRNA sequences found in GenBank, were used to trigger the PCR amplification of untranslated or intronic gene regions.
  • The researchers used two distinct bacterial artificial chromosome (BAC) libraries of the horse, which were assessed with PCR to identify clones containing any of the 13 Type I loci under investigation.
  • The use of BAC clones as probes in dual colour FISH for determining their accurate chromosomal origin.
  • The mapping of four genes using a somatic cell hybrid panel is noted. This technique utilizes hybrid cells created from two distinct organisms to study and map genes on chromosomes.

Findings

  • Among the 13 genes studied, nine were identified within the two horse BAC libraries.
  • All the chromosomal assignments of these genes, except for one, were consistent with the existing human-horse ZOO-FISH data.
  • However, the CLU gene was found, through synteny mapping, on chromosome ECA2, while the previous human-horse ZOO-FISH data had placed it on ECA9.
  • The positioning of the IL1RN gene was instrumental in analysing the conservation of gene order between HSA2 (a human chromosome) and ECA15 (a horse chromosome), highlighting an event of inversion in the evolutionary history of these two homologous chromosomes.

Significance

The research contributed significant new insights and details to the equine comparative map. It shed light on the feasibility of applying FISH and somatic cell hybrid analyses in studying horse genes and demonstrates the potential for unearthing new information about horse genetics through these methods.

Cite This Article

APA
Lindgren G, Breen M, Godard S, Bowling A, Murray J, Scavone M, Skow L, Sandberg K, Guérin G, Binns M, Ellegren H. (2001). Mapping of 13 horse genes by fluorescence in-situ hybridization (FISH) and somatic cell hybrid analysis. Chromosome Res, 9(1), 53-59. https://doi.org/10.1023/a:1026743700819

Publication

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology
ISSN: 0967-3849
NlmUniqueID: 9313452
Country: Netherlands
Language: English
Volume: 9
Issue: 1
Pages: 53-59

Researcher Affiliations

Lindgren, G
  • Department of Evolutionary Biology, Norbyvägen, Uppsala University, Sweden. gabriella.lindgren@ebc.uu.se
Breen, M
    Godard, S
      Bowling, A
        Murray, J
          Scavone, M
            Skow, L
              Sandberg, K
                Guérin, G
                  Binns, M
                    Ellegren, H

                      MeSH Terms

                      • Animals
                      • Chromosome Mapping
                      • Gene Library
                      • Horses / genetics
                      • Hybrid Cells
                      • In Situ Hybridization, Fluorescence
                      • Metaphase
                      • Polymerase Chain Reaction

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                      Citations

                      This article has been cited 1 times.
                      1. Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
                        doi: 10.1007/s10577-008-1204-zpubmed: 18274866google scholar: lookup
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