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International journal of molecular sciences2021; 22(2); doi: 10.3390/ijms22020580

Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids.

Abstract: Articular cartilage experiences mechanical constraints leading to chondral defects that inevitably evolve into osteoarthritis (OA), because cartilage has poor intrinsic repair capacity. Although OA is an incurable degenerative disease, several dietary supplements may help improve OA outcomes. In this study, we investigated the effects of Dielen® hydrolyzed fish collagens from skin (Promerim®30 and Promerim®60) and cartilage (Promerim®40) to analyze the phenotype and metabolism of equine articular chondrocytes (eACs) cultured as organoids. Here, our findings demonstrated the absence of cytotoxicity and the beneficial effect of Promerim® hydrolysates on eAC metabolic activity under physioxia; further, Promerim®30 also delayed eAC senescence. To assess the effect of Promerim® in a cartilage-like tissue, eACs were cultured as organoids under hypoxia with or without BMP-2 and/or IL-1β. In some instances, alone or in the presence of IL-1β, Promerim®30 and Promerim®40 increased protein synthesis of collagen types I and II, while decreasing transcript levels of proteases involved in OA pathogenesis, namely Htra1, and the metalloproteinases Mmp1-3, Adamts5, and Cox2. Both Promerim® hydrolysates also decreased Htra1 protein amounts, particularly in inflammatory conditions. The effect of Promerim® was enhanced under inflammatory conditions, possibly due to a decrease in the synthesis of inflammation-associated molecules. Finally, Promerim® favored in vitro repair in a scratch wound assay through an increase in cell proliferation or migration. Altogether, these data show that Promerim®30 and 40 hold promise as dietary supplements to relieve OA symptoms in patients and to delay OA progression.
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Publication Date: 2021-01-08 PubMed ID: 33430111PubMed Central: PMC7826754DOI: 10.3390/ijms22020580Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of hydrolyzed marine collagen supplements on equine articular chondrocytes (essentially a specialized type of cell in horses’ joint cartilage), with a focus on how they may help alleviate symptoms and slow progression of osteoarthritis, a common degenerative joint disease.

Research Background

  • The study draws attention towards osteoarthritis (OA), a degenerative disease affecting joint cartilage that often results from underlying defects in the chondral (cartilage) tissue. Due to the lack of significant self-healing capabilities of this tissue, OA is essentially incurable, leading to a growing interest in potential dietary supplements that might help manage the disease’s symptoms.

Methodology and Materials

  • Three hydrolyzed fish collagen variants, namely Promerim30, Promerim40 and Promerim60, derived from fish skin and cartilage, are examined in this study.
  • The researchers studied the impact of these collagen supplements on equine articular chondrocytes (eACs) — cells that maintain cartilage structure in joints — by cultivating them in controlled environments (organoids).

Key Findings

  • The researchers found that the hydrolyzed collagens were not cytotoxic and boosted metabolic activity in eACs under specific oxygen conditions. In particular, Promerim30 was observed to delay cell aging.
  • When organoids were exposed to hypoxia (low oxygen conditions), the Promerim30 and Promerim40 supplements increased the synthesis of specific collagen proteins and reduced the transcription levels of enzymes linked to OA pathogenesis.
  • Promerim30 and 40, especially under inflammatory conditions, significantly reduced the amount of a protein called Htra1, which is associated with cartilage degradation in OA.
  • The study showed that the collagens, under inflammatory conditions, could potentially reduce the synthesis of molecules related to inflammation.
  • Furthermore, these collagens showed promising results in a scratch wound assay, implying that they might facilitate cartilage repair by promoting cell proliferation and migration.

Implications of the Study

  • The research implies that these specific marine collagen hydrolysates could be effective dietary supplements to relieve symptoms of OA.
  • Given their positive impact on cartilage cell metabolism, protein production, and inflammation control, both Promerim30 and Promerim40 may also contribute to slowing the progression of OA.

Cite This Article

APA
Bourdon B, Contentin R, Cassé F, Maspimby C, Oddoux S, Noël A, Legendre F, Gruchy N, Galéra P. (2021). Marine Collagen Hydrolysates Downregulate the Synthesis of Pro-Catabolic and Pro-Inflammatory Markers of Osteoarthritis and Favor Collagen Production and Metabolic Activity in Equine Articular Chondrocyte Organoids. Int J Mol Sci, 22(2). https://doi.org/10.3390/ijms22020580

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 22
Issue: 2

Researcher Affiliations

Bourdon, Bastien
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
  • Dielen Laboratory, 50110 Tourlaville, France.
Contentin, Romain
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
Cassé, Frédéric
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
Maspimby, Chloé
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
Oddoux, Sarah
  • Dielen Laboratory, 50110 Tourlaville, France.
Noël, Antoine
  • Dielen Laboratory, 50110 Tourlaville, France.
Legendre, Florence
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
Gruchy, Nicolas
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.
  • Department of Genetics, Normandy Center for Genomic and Personalized Medicine, Caen University Hospital, 14000 Caen, France.
Galéra, Philippe
  • Normandie University, UNICAEN, BIOTARGEN, 14000 Caen, France.

MeSH Terms

  • Animals
  • Cartilage, Articular / drug effects
  • Cartilage, Articular / growth & development
  • Cell Differentiation / drug effects
  • Cells, Cultured
  • Chondrocytes / drug effects
  • Collagen / biosynthesis
  • Horses
  • Humans
  • Inflammation / drug therapy
  • Inflammation / genetics
  • Inflammation / pathology
  • Organoids / drug effects
  • Organoids / growth & development
  • Osteoarthritis / drug therapy
  • Skin / chemistry

Conflict of Interest Statement

Mr. Bourdon was employed in the framework of a CIFRE thesis co-financed by L’Asssociation Nationale de la Recherche et de la Techonologie (ANRT) and by the Dielen laboratory which provided the marine hydrolysates investigated in this study. S. Oddoux and A. Noel are employees of the Dielen laboratory. None of the academic authors received any remuneration from the company.

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