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Matrix-Induced Autologous Chondrocyte Implantation (MACI) Using a Cell-Seeded Collagen Membrane Improves Cartilage Healing in the Equine Model.
Abstract: Autologous chondrocyte implantation (ACI) using a collagen scaffold (matrix-induced ACI; MACI) is a next-generation approach to traditional ACI that provides the benefit of autologous cells and guided tissue regeneration using a biocompatible collagen scaffold. The MACI implant also has inherent advantages including surgical implantation via arthroscopy or miniarthrotomy, the elimination of periosteal harvest, and the use of tissue adhesive in lieu of sutures. This study evaluated the efficacy of the MACI implant in an equine full-thickness cartilage defect model at 1 year. Methods: Autologous chondrocytes were seeded onto a collagen type-I/III membrane and implanted into one of two 15-mm defects in the femoral trochlear ridge of 24 horses. Control defects either were implanted with cell-free collagen type-I/III membrane (12 horses) or were left ungrafted as empty defects (12 horses). An additional 3 horses had both 15-mm defects remain empty as nonimplanted joints. The repair was scored by second-look arthroscopy (12 weeks), and necropsy examination (53 weeks). Healing was assessed by arthroscopic scoring, gross assessment, histology and immunohistology, cartilage matrix component assay, and gene expression determination. Toxicity was examined by prostaglandin E2 formation in joint fluid, and lymph node morphology combined with histologic screening of organs. Results: MACI-implanted defects had improved gross healing and composite histologic scores, as well as increases in chondrocyte predominance, toluidine blue-stained matrix, and collagen type-II content compared with scaffold-only implanted or empty defects. There was minimal evidence of reaction to the implant in the synovial membrane (minor perivascular cuffing), subchondral bone, or cartilage. There were no adverse clinical effects, signs of organ toxicity, or evidence of chondrocytes or collagen type-I/III membrane in draining lymph nodes. Conclusions: The MACI implant appeared to improve cartilage healing in a critical-sized defect in the equine model compared with collagen matrix alone. Conclusions: These results indicate that the MACI implant is quick to insert, provides chondrocyte security in the defect, and improves cartilage healing compared with ACI.
Publication Date: 2017-12-06 PubMed ID: 29206788DOI: 10.2106/JBJS.16.00603Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
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The researchers investigated if the use of Matrix-Induced Autologous Chondrocyte Implantation (MACI) using a cell-seeded collagen membrane would improve the healing of cartilage in horses. The study found that the MACI implantation not only improved cartilage repair but also has added advantages such as simpler surgical installation and the elimination of periosteal harvest.
Methods
- The study used 24 horses, where autologous chondrocytes were seeded onto a collagen type-I/III membrane and implanted into one of two 15-mm defects in the femoral trochlear ridge.
- The control defects were either implanted with cell-free collagen type-I/III membrane (12 horses) or were left ungrafted as empty defects (12 horses).
- An additional 3 horses had both 15-mm defects remain empty as nonimplanted joints.
- Healing was examined using arthroscopic scoring, gross assessment, histology and immunohistology, cartilage matrix component assay, and gene expression determination.
- Toxicity was assessed by examining prostaglandin E2 formation in joint fluid, and lymph node morphology combined with a histologic screening of organs.
Results
- MACI-implanted defects showed improved gross healing and composite histologic scores.
- There was an increase in chondrocyte predominance, toluidine blue-stained matrix, and collagen type-II content compared with scaffold-only implanted or empty defects.
- Minimal evidence was found on reaction to the implant in the synovial membrane or the subchondral bone or cartilage.
- There were no adverse clinical effects, signs of organ toxicity, or evidence of chondrocytes or collagen type-I/III membrane in draining lymph nodes.
Conclusion
- MACI implant appeared to improve cartilage healing in a critical-sized defect in the equine model compared with collagen matrix alone.
- The MACI implant is quicker to insert, provides chondrocyte security in the defect, and improves cartilage healing compared with Autologous Chondrocyte Implantation (ACI).
Cite This Article
APA
Nixon AJ, Sparks HD, Begum L, McDonough S, Scimeca MS, Moran N, Matthews GL.
(2017).
Matrix-Induced Autologous Chondrocyte Implantation (MACI) Using a Cell-Seeded Collagen Membrane Improves Cartilage Healing in the Equine Model.
J Bone Joint Surg Am, 99(23), 1987-1998.
https://doi.org/10.2106/JBJS.16.00603 Publication
Researcher Affiliations
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences (A.J.N., H.D.S., L.B., and M.S.S.), and Department of Biomedical Sciences, College of Veterinary Medicine (S.M.), Cornell University, Ithaca, New York.
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences (A.J.N., H.D.S., L.B., and M.S.S.), and Department of Biomedical Sciences, College of Veterinary Medicine (S.M.), Cornell University, Ithaca, New York.
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences (A.J.N., H.D.S., L.B., and M.S.S.), and Department of Biomedical Sciences, College of Veterinary Medicine (S.M.), Cornell University, Ithaca, New York.
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences (A.J.N., H.D.S., L.B., and M.S.S.), and Department of Biomedical Sciences, College of Veterinary Medicine (S.M.), Cornell University, Ithaca, New York.
- Comparative Orthopaedics Laboratory, Department of Clinical Sciences (A.J.N., H.D.S., L.B., and M.S.S.), and Department of Biomedical Sciences, College of Veterinary Medicine (S.M.), Cornell University, Ithaca, New York.
- Histogenics Corporation, Waltham, Massachusetts.
- Histogenics Corporation, Waltham, Massachusetts.
MeSH Terms
- Animals
- Arthroscopy
- Cartilage, Articular / surgery
- Cell Transplantation / methods
- Chondrocytes / transplantation
- Collagen Type I / administration & dosage
- Collagen Type I / pharmacology
- Collagen Type III
- Disease Models, Animal
- Guided Tissue Regeneration / methods
- Horses
- Patellofemoral Joint / surgery
- Transplantation, Autologous
- Wound Healing / physiology
Citations
This article has been cited 17 times.- Shajib MS, Futrega K, Franco RAG, McKenna E, Guillesser B, Klein TJ, Crawford RW, Doran MR. Method for manufacture and cryopreservation of cartilage microtissues. J Tissue Eng 2023 Jan-Dec;14:20417314231176901.
- Rashidi A, Theruvath AJ, Huang CH, Wu W, Mahmoud EE, Jesu Raj JG, Marycz K, Daldrup-Link HE. Vascular injury of immature epiphyses impair stem cell engraftment in cartilage defects. Sci Rep 2022 Jul 9;12(1):11696.
- Voga M, Majdic G. Articular Cartilage Regeneration in Veterinary Medicine. Adv Exp Med Biol 2022;1401:23-55.
- Fernandes TL, de Faria RR, Gonzales MA, Sherman SL, Goldchmit S, Fleury A. Innovation in Orthopaedics: Part 2-How to Translate Ideas and Research into Clinical Practice. Curr Rev Musculoskelet Med 2022 Apr;15(2):150-155.
- Xie J, Wang W, Zhao R, Lu W, Chen L, Su W, Zeng M, Hu Y. Fabrication and characterization of microstructure-controllable COL-HA-PVA hydrogels for cartilage repair. J Mater Sci Mater Med 2021 Aug 18;32(9):100.
- Aldrich ED, Cui X, Murphy CA, Lim KS, Hooper GJ, McIlwraith CW, Woodfield TBF. Allogeneic mesenchymal stromal cells for cartilage regeneration: A review of in vitro evaluation, clinical experience, and translational opportunities. Stem Cells Transl Med 2021 Nov;10(11):1500-1515.
- Nouri Barkestani M, Naserian S, Uzan G, Shamdani S. Post-decellularization techniques ameliorate cartilage decellularization process for tissue engineering applications. J Tissue Eng 2021 Jan-Dec;12:2041731420983562.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases. Animals (Basel) 2021 Jan 19;11(1).
- Lv X, Sun C, Hu B, Chen S, Wang Z, Wu Q, Fu K, Xia Z, Shao Z, Wang B. Simultaneous Recruitment of Stem Cells and Chondrocytes Induced by a Functionalized Self-Assembling Peptide Hydrogel Improves Endogenous Cartilage Regeneration. Front Cell Dev Biol 2020;8:864.
- Peal BT, Gagliardi R, Su J, Fortier LA, Delco ML, Nixon AJ, Reesink HL. Synovial fluid lubricin and hyaluronan are altered in equine osteochondral fragmentation, cartilage impact injury, and full-thickness cartilage defect models. J Orthop Res 2020 Aug;38(8):1826-1835.
- Fugazzola MC, van Weeren PR. Surgical osteochondral defect repair in the horse-a matter of form or function?. Equine Vet J 2020 Jul;52(4):489-499.
- Li X, Liang Y, Xu X, Xiong J, Ouyang K, Duan L, Wang D. Cell-to-Cell Culture Inhibits Dedifferentiation of Chondrocytes and Induces Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cells. Biomed Res Int 2019;2019:5871698.
- Yoon TH, Jung M, Choi CH, Kim HS, Lee YH, Choi YS, Kim SJ, Kim SH. Arthroscopic gel-type autologous chondrocyte implantation presents histologic evidence of regenerating hyaline-like cartilage in the knee with articular cartilage defect. Knee Surg Sports Traumatol Arthrosc 2020 Mar;28(3):941-951.
- Cucchiarini M, Madry H. Biomaterial-guided delivery of gene vectors for targeted articular cartilage repair. Nat Rev Rheumatol 2019 Jan;15(1):18-29.
- Żylińska B, Silmanowicz P, Sobczyńska-Rak A, Jarosz Ł, Szponder T. Treatment of Articular Cartilage Defects: Focus on Tissue Engineering. In Vivo 2018 Nov-Dec;32(6):1289-1300.
- Palhares GM, Retzky JS, Coxe F, Hinkley P, Rizy ME, Neijna AG, Gomoll AH, Strickland SM. Patellofemoral Joint Chondral Defects Treated With Third-Generation Matrix-Induced Autologous Chondrocyte Implantation on Porcine Collagen Membrane: Minimum 2-Year Follow-up. Orthop J Sports Med 2025 May;13(5):23259671251341474.
- Patel K, Ozhava D, Mao Y. Expansion and Delivery of Human Chondrocytes on Gelatin-Based Cell Carriers. Gels 2025 Mar 13;11(3).
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