Analyze Diet
Journal of animal science2010; 88(6); 2038-2044; doi: 10.2527/jas.2009-2693

Maximal lactate steady state during exercise in blood of horses.

Abstract: The speed producing the maximal lactate steady state (maxLASS) is supposed to be the optimal speed to condition for endurance. The maxLASS was defined as the maximal speed at which the blood lactate concentration ([LA]) between the 5th and the 25th min of continuous exercise did not increase by more than 1 mmol/L. According to the aerobic-anaerobic lactate threshold concept determined in humans, maxLASS corresponds to v(4) [speed in a standardized exercise test (SET) shown to produce an [LA] of 4 mmol/L; generalized to v(i) for the speed producing an [LA] of i mmol/L]. Four Thoroughbreds were submitted to a treadmill-based SET to determine their blood lactate-running speed (BLRS) relationship and calculate the individual v(1.5), v(2), v(2.5), v(3), and v(4) values (velocities run under defined conditions inducing 1.5, 2, 2.5, 3, and 4 mmol/L of blood LA). Afterward, horses ran on the treadmill for 40 min at their v(1.5), v(2), and v(2.5) every 3 d. Another 14 horses were submitted to SET in the field to determine their BLRS relationships and to calculate their v(2). The day after the SET, these horses ran once between 15 and 30 min at their v(2). In the horses that ran on the treadmill, maxLASS only occurred when running at their v(1.5). Blood [LA] did not increase by more than 1 mmol/L between the 10th min and the end of exercise for all the horses that ran in the field at their v(2.) These data indicate that maxLASS of horses is not greater than v(2) and therefore less than in running humans.
Publication Date: 2010-02-26 PubMed ID: 20190168DOI: 10.2527/jas.2009-2693Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study examined the maximum lactate steady state (maxLASS) during exercise in the blood of four Thoroughbred horses utilizing a treadmill exercise protocol, as well as the maxLASS of fourteen other horses under field conditions. It concluded that the maxLASS of horses was found to not exceed a speed inducing a blood lactate concentration of 2 mmol/L (v2), which is lower compared to humans.

Research Design and Methodology

  • This study involves an exploration of the maximal lactate steady state (maxLASS), defined as maximum speed during which the blood lactate concentration ([LA]) does not increase by more than 1 mmol/L between the 5th and 25th minutes of continuous exercise.
  • Four Thoroughbred horses underwent a treadmill-based Standardized Exercise Test (SET) to determine their Blood Lactate-Running Speed (BLRS) relationship and calculate individual v(1.5), v(2), v(2.5), v(3), and v(4) values. These values correspond to the speeds that produce blood LA of 1.5, 2, 2.5, 3, and 4 mmol/L respectively.
  • Following this, horses were made to run on the treadmill for 40 minutes at their v(1.5), v(2), and v(2.5) speeds, the practice being repeated every 3 days.
  • In a separate part of the study, fourteen other horses underwent a SET in open field conditions to ascertain their BLRS relationship and calculate their v(2).
  • Afterwards these fourteen horses ran between 15 and 30 minutes at their v(2) speed.

Research Findings

  • In the treadmill test, maxLASS was achieved only at v(1.5) speeds.
  • For the horses tested in open field conditions, blood LA concentration did not increase more than 1 mmol/L between the 10th minute to the end of the exercise when they ran at their v(2) speeds.
  • Overall, the study demonstrated that the maxLASS of horses is not higher than v(2), suggesting that this value is lower compared to maximum lactate steady state observations in running humans.

Significance of the Study

  • The research linked the concept of maxLASS, originally constituted for humans, to measure the aerobic-anaerobic lactate threshold in horses.
  • The findings are significant for the physical conditioning and athletic training of horses, especially those participating in endurance-related sports.
  • As the maxLASS of horses is found to be lower compared to humans, this differentiating factor should be considered in designing exercise and fitness regimes for equine athletes.

Cite This Article

APA
Lindner AE. (2010). Maximal lactate steady state during exercise in blood of horses. J Anim Sci, 88(6), 2038-2044. https://doi.org/10.2527/jas.2009-2693

Publication

ISSN: 1525-3163
NlmUniqueID: 8003002
Country: United States
Language: English
Volume: 88
Issue: 6
Pages: 2038-2044

Researcher Affiliations

Lindner, A E
  • Arbeitsgruppe Pferd, D-52428 Juelich, Germany.

MeSH Terms

  • Animals
  • Exercise Test / veterinary
  • Horses / physiology
  • Lactic Acid / blood
  • Physical Conditioning, Animal / physiology

Citations

This article has been cited 9 times.
  1. Zuluaga-Cabrera AM, Barbosa da Costa G, Martinez ID, Arias MP. Defining the Heart Rate Zone Corresponding to the Lactate Threshold in Colombian Paso Horses. Animals (Basel) 2025 Nov 17;15(22).
    doi: 10.3390/ani15223308pubmed: 41302017google scholar: lookup
  2. Liedtke AM, Meijer H, Horstmann S, von Reitzenstein C, Rump I, Kirsch K. Modelling Energy Demands of Cross-Country Tests in 2-Star to 5-Star Eventing Competitions. Animals (Basel) 2025 Jun 17;15(12).
    doi: 10.3390/ani15121775pubmed: 40564327google scholar: lookup
  3. Navas de Solis C, Ramseyer A, Stefanovski D, Haughan J, Solomon CJ, Kirsch K. Association of heart rate variability, exercise intensity and exercising arrhythmias with competition results in eventing horses. Equine Vet J 2025 Nov;57(6):1446-1456.
    doi: 10.1111/evj.14491pubmed: 40008417google scholar: lookup
  4. Ramos GV, Santos MM, Gava FN, de Lacerda-Neto JC. Effects of conditioning on the left ventricular function of young purebred Arabian horses. PLoS One 2024;19(6):e0304724.
    doi: 10.1371/journal.pone.0304724pubmed: 38829873google scholar: lookup
  5. Ramos GV, Titotto AC, da Costa GB, Ferraz GC, de Lacerda-Neto JC. Determination of speed and assessment of conditioning in horses submitted to a lactate minimum test-alternative approaches. Front Physiol 2024;15:1324038.
    doi: 10.3389/fphys.2024.1324038pubmed: 38725567google scholar: lookup
  6. Santos MM, Ramos GV, de Figueiredo IM, Silva TCBV, Lacerda-Neto JC. Cardiac Changes after Lactate-Guided Conditioning in Young Purebred Arabian Horses. Animals (Basel) 2023 May 29;13(11).
    doi: 10.3390/ani13111800pubmed: 37889733google scholar: lookup
  7. Siegers E, van Wijk E, van den Broek J, Sloet van Oldruitenborgh-Oosterbaan M, Munsters C. Longitudinal Training and Workload Assessment in Young Friesian Stallions in Relation to Fitness: Part 1. Animals (Basel) 2023 Feb 16;13(4).
    doi: 10.3390/ani13040689pubmed: 36830476google scholar: lookup
  8. De Maré L, Boshuizen B, Vidal Moreno de Vega C, de Meeûs C, Plancke L, Gansemans Y, Van Nieuwerburgh F, Deforce D, de Oliveira JE, Hosotani G, Oosterlinck M, Delesalle C. Profiling the Aerobic Window of Horses in Response to Training by Means of a Modified Lactate Minimum Speed Test: Flatten the Curve. Front Physiol 2022;13:792052.
    doi: 10.3389/fphys.2022.792052pubmed: 35392373google scholar: lookup
  9. Almeida JA, Petriz Bde A, da Costa Gomes CP, Pereira RW, Franco OL. Assessment of maximal lactate steady state during treadmill exercise in SHR. BMC Res Notes 2012 Nov 30;5:661.
    doi: 10.1186/1756-0500-5-661pubmed: 23194345google scholar: lookup