MCT1 and CD147 gene polymorphisms in standardbred horses.
Abstract: Transport of lactate across membranes is facilitated by proton-monocarboxylate transporters (MCT). The most widely distributed isoform is MCT1, which needs an ancillary protein CD147. Studies on erythrocytes have shown that high activity of MCT1 is inherited as the dominant allele and that activity is regulated through CD147. Mutations of human MCT1 have been described that appear to impair lactate transport in muscles and cause exertional rhabdomyolysis. There are no reports of this potential relationship in the horse. Objective: To obtain sequences of equine MCT1 and CD147 to examine differences between horses with high and low lactate transport activity in their erythrocytes. Methods: Muscle biopsy samples were taken from 3 healthy Standardbred horses and from 7 horses which according to the owners had signs of myopathy after intense exercise. DNA and RNA were isolated and PCR analysis and sequencing performed. Results: Currently, PCR fragments covering 100% of MCT1 and 70% of CD147 coding region are retained and sequence analysis has demonstrated one single nucleotide polymorphism (SNP) in the C-terminal area of MCT1 and one SNP in the extracellular domain of CD147. Both cause an amino acid change. The SNPs found are not related to lactate transport activity in erythrocytes or signs of myopathy. Conclusions: More samples need to be analysed to make conclusions on the significance of the polymorphisms found. Furthermore, full sequence coverage of the coding region of CD147 is needed. Conclusions: The molecular probes produced could be used as tools to study gene regulation of lactate transport.
Publication Date: 2007-04-04 PubMed ID: 17402440DOI: 10.1111/j.2042-3306.2006.tb05561.xGoogle Scholar: Lookup
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- Journal Article
Summary
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The research studies the genetic differences in horses that may affect lactate transport in their muscles. It specifically focuses on the variants of MCT1 and CD147 genes which facilitate this process. The results found some genetic differences but these do not seem to be linked to muscle health or lactate transport.
Objective of the Research
- The research aimed at exploring the genetic differences within the MCT1 and CD147 genes in Standardbred horses. These two genes play a critical role in the lactate transport process which is crucial for muscle function. The study sought to examine if any variations in these sequences were connected to higher or lower lactacyte transport activities or signs of myopathy.
Methodology
- The research was conducted using muscle biopsy samples taken from three healthy horses and seven horses with myopathy symptoms following intense exercise as reported by their owners.
- The DNA and RNA were isolated from these samples and subjected to PCR analysis and sequencing to decipher the genetic sequences of MCT1 and CD147 in these horses.
Findings
- From the sequencing, PCR fragments covering 100% of MCT1 and 70% of CD147 coding region were obtained.
- The analysis revealed a single nucleotide polymorphism (SNP) each in MCT1 and CD147 genes. Both SNPs resulted in an amino acid alteration.
- However, these SNPs did not seem to be responsible for variations in lactate transport or signs of myopathy, contrary to what was initially assumed.
Conclusions
- The researchers conclude that extensive data from more samples is necessary for deriving any substantial conclusions regarding the significance of the SNPs identified.
- Furthermore, they postulated that full sequence coverage of the CD147 coding region could be a potential area of exploration.
- Finally, the molecular probes created during this research could potentially be employed to study the gene regulation aspects of lactate transport in the future.
Cite This Article
APA
Reeben M, Koho NM, Raekallio M, Hyyppä S, Pösö AR.
(2007).
MCT1 and CD147 gene polymorphisms in standardbred horses.
Equine Vet J Suppl(36), 322-325.
https://doi.org/10.1111/j.2042-3306.2006.tb05561.x Publication
Researcher Affiliations
- Department of Basic Veterinary Sciences and tDepartment of Clinical Veterinary Sciences, POB 66, 00014 University of Helsinki, Finland.
MeSH Terms
- Animals
- Basigin / genetics
- Basigin / metabolism
- Erythrocytes / metabolism
- Female
- Horses
- Lactates / metabolism
- Male
- Monocarboxylic Acid Transporters / genetics
- Monocarboxylic Acid Transporters / metabolism
- Muscle, Skeletal / metabolism
- Physical Conditioning, Animal / physiology
- Polymerase Chain Reaction / methods
- Polymerase Chain Reaction / veterinary
- Polymorphism, Single Nucleotide
Citations
This article has been cited 2 times.- Chowdhary BP, Raudsepp T. The horse genome derby: racing from map to whole genome sequence. Chromosome Res 2008;16(1):109-27.
- Yang XM, Bian H, Chen ZN. CD147/Basigin: From Integrative Molecular Hub to Translational Therapeutic Target. Adv Sci (Weinh) 2026 Feb;13(8):e18884.
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