Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases – an initial case study.
Abstract: The aim of this study was to develop a specific myeloperoxidase (MPO) activity assay in the synovial fluid of horses and investigate whether MPO activity is increased in different forms of joint diseases. Synovial fluid samples were taken from affected joints from horses with osteoarthritis, chronic non-septic arthritis and septic arthritis, and from healthy control horses. MPO activity was measured using a specific modified o-dianisidine-assay containing 4-aminobenzoic acid hydrazide as a potent and specific inhibitor of the MPO. This assay is characterized by high reproducibility. The results reveal only a slight elevation of MPO activity in the synovial fluid of horses with osteoarthritis and chronic non-septic arthritis. However, in the cases of septic arthritis a significant increase in MPO activity was found when compared to the controls. In conclusion the first field study suggests that synovial fluid MPO may be used as a marker for septic arthritis in horses.
Publication Date: 2007-08-06 PubMed ID: 17675122DOI: 10.1016/j.rvsc.2007.06.006Google Scholar: Lookup
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- Journal Article
Summary
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This research article explores a new method of testing for different forms of joint disease in horses by measuring the myeloperoxidase (MPO) activity in their synovial fluid. The study found that MPO levels significantly increased in cases of septic arthritis, suggesting it may serve as a marker for the disease.
Objective and Methodology
- The purpose of this study was two-fold. Firstly, it sought to develop a reliable method for measuring the activity of myeloperoxidase (MPO), an enzyme produced by certain white blood cells, in the synovial fluid of horses. Secondly, it aimed to determine whether MPO activity levels could indicate the presence of various forms of joint diseases.
- The researchers extracted synovial fluid samples from horses suffering from different joint diseases such as osteoarthritis, chronic non-septic arthritis, and septic arthritis, as well as healthy horses for control.
- MPO activity in the samples was determined using a specifically modified version of an o-dianisidine-assay. This variation of the testing method included 4-aminobenzoic acid hydrazide, a strong and specific inhibitor of MPO, to enhance the accuracy of the results.
Findings and Conclusion
- The outcomes demonstrated that MPO activity was slightly elevated in horses with osteoarthritis and chronic non-septic arthritis compared to healthy horses.
- However, the MPO activity was significantly higher in cases of septic arthritis, suggesting a strong correlation between the presence of this particular disease and elevated MPO levels.
- Therefore, the researchers concluded that the assay method they developed could be used to accurately measure MPO activity in a horse’s synovial fluid and that an increased MPO activity level could serve as an indicator of septic arthritis in horses. This finding implies that the modified MPO assay could be an important tool in diagnosing this equine condition and informing treatment strategies.
Cite This Article
APA
Fietz S, Bondzio A, Moschos A, Hertsch B, Einspanier R.
(2007).
Measurement of equine myeloperoxidase (MPO) activity in synovial fluid by a modified MPO assay and evaluation of joint diseases – an initial case study.
Res Vet Sci, 84(3), 347-353.
https://doi.org/10.1016/j.rvsc.2007.06.006 Publication
Researcher Affiliations
- Institute of Veterinary Biochemistry, Freie Universität Berlin, Oertzenweg 19b, D-14163 Berlin, Germany.
MeSH Terms
- Animals
- Arthritis / enzymology
- Arthritis / veterinary
- Arthritis, Infectious / enzymology
- Arthritis, Infectious / veterinary
- Horse Diseases / enzymology
- Horses
- Joint Diseases / enzymology
- Joint Diseases / veterinary
- Kinetics
- Knee Joint / enzymology
- Osteoarthritis / enzymology
- Osteoarthritis / veterinary
- Peroxidase / metabolism
- Reference Values
- Synovial Fluid / enzymology
Citations
This article has been cited 9 times.- Zhou L, Zhang D, Kong L, Xu X, Gong D. Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model. J Transl Med 2022 Nov 22;20(1):538.
- Hoyo J, Bassegoda A, Ferreres G, Hinojosa-Caballero D, Gutiérrez-Capitán M, Baldi A, Fernández-Sánchez C, Tzanov T. Rapid Colorimetric Detection of Wound Infection with a Fluidic Paper Device. Int J Mol Sci 2022 Aug 15;23(16).
- Haralambus R, Florczyk A, Sigl E, Gültekin S, Vogl C, Brandt S, Schnierer M, Gamerith C, Jenner F. Detection of synovial sepsis in horses using enzymes as biomarkers. Equine Vet J 2022 May;54(3):513-522.
- Torres-Manzo AP, Franco-Colín M, Blas-Valdivia V, Pineda-Reynoso M, Cano-Europa E. Hypothyroidism Causes Endoplasmic Reticulum Stress in Adult Rat Hippocampus: A Mechanism Associated with Hippocampal Damage. Oxid Med Cell Longev 2018;2018:2089404.
- Li X, Zhou X, Ye Y, Li Y, Li J, Privratsky B, Wu E, Gao H, Huang C, Wu M. Lyn regulates inflammatory responses in Klebsiella pneumoniae infection via the p38/NF-κB pathway. Eur J Immunol 2014 Mar;44(3):763-73.
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- Verwilghen DR, Martens A, Busschers E, Franck T, Deberg M, Henrotin Y, Vanderheyden L, Serteyn D. Coll2-1, Coll2-1NO2 and myeloperoxidase concentrations in the synovial fluid of equine tarsocrural joints affected with osteochondrosis. Vet Res Commun 2011 Oct;35(7):401-8.
- Ding F, Song JH, Jung JY, Lou L, Wang M, Charles L, Westover A, Smith PL, Pino CJ, Buffington DA, Humes HD. A biomimetic membrane device that modulates the excessive inflammatory response to sepsis. PLoS One 2011 Apr 14;6(4):e18584.
- Nocera I, Sgorbini M, Meucci V, Gracia-Calvo LA, Tapio H, Camisi M, Sala G, Citi S. Procalcitonin and carbonylated protein concentrations in equine synovial fluid. Vet Res Commun 2024 Apr;48(2):1263-1269.
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