Mechanism of cell death in inflamed superficial digital flexor tendon in the horse.
Abstract: The aim of the present study was to clarify the presence and determine the role of apoptosis in the degenerative process of the superficial digital flexor tendon (SDFT) in the horse. Samples were obtained from normal and inflamed SDFTs of horses. To detect apoptosis and to identify apoptotic cells, the samples were subjected to immunohistochemical labelling and Western blot analysis. Although a large number of cells in degenerate areas showed positive reactions with caspase-3 and single stranded DNA antibodies, cells in normal tendon samples showed very weak reactions. Excessive apoptosis was confirmed by the results of Western blot analysis, which showed a significant increase in activated caspase-3 protein in the inflamed SDFTs, suggesting that apoptosis occurred in the tendinocytes via a caspase-3-dependent pathway. This is the first report of excessive apoptosis in inflamed SDFT of the horse. The results indicate that apoptosis may play an important role in the process of degeneration of the tendon as well as other tissues.
Publication Date: 2005-01-05 PubMed ID: 15629479DOI: 10.1016/j.jcpa.2004.06.006Google Scholar: Lookup
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- Journal Article
Summary
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This research aims to understand the role of cell death (apoptosis) in the degenerative process of a specific tendon (Superficial Digital Flexor Tendon, SDFT) in horses, especially when it is inflamed. Researchers have found significant evidence of apoptosis in inflamed SDFTs, pointing toward it being a critical factor in tendon degeneration.
Objective of the Study:
- The study set out to identify the presence and role of apoptosis (programmed cell death) in the process of degradation of the superficial digital flexor tendon (SDFT) in horses when the tendon is inflamed.
Methodology:
- The study was carried out using samples from normal and inflamed SDFTs in horses.
- The researchers used processes like immunohistochemical labelling and Western blot analysis to detect signs of apoptosis and to identify apoptotic cells.
Findings:
- In areas of the tendon that were degenerating, a large number of cells showed positive reactions to caspase-3, an enzyme that plays a vital role in the process of apoptosis, and to single-stranded DNA antibodies. Such reactions were very weak in cells from normal tendon samples.
- Through Western blot analysis, the researchers were able to establish that there was significant increase in the presence of activated caspase-3 protein in the inflamed SDFTs. This indicated that the apoptosis happened via a caspase-3-dependent pathway.
Significance of the Study:
- This study is the first to find evidence of extensive apoptosis in inflamed SDFTs in horses.
- The findings suggest that apoptosis could potentially play a key role in the process of degeneration of not only the tendon but possibly other tissues as well.
Cite This Article
APA
Hosaka Y, Teraoka H, Yamamoto E, Ueda H, Takehana K.
(2005).
Mechanism of cell death in inflamed superficial digital flexor tendon in the horse.
J Comp Pathol, 132(1), 51-58.
https://doi.org/10.1016/j.jcpa.2004.06.006 Publication
Researcher Affiliations
- Department of Veterinary Anatomy, School of Veterinary Medicine, Rakuno Gakuen University, 582 Bunkyodai-Midorimachi, Ebetsu, Hokkaido 069-8501, Japan.
MeSH Terms
- Animals
- Apoptosis / physiology
- Blotting, Western / veterinary
- Caspase 3
- Caspases / analysis
- DNA Fragmentation
- DNA, Single-Stranded / analysis
- Female
- Forelimb / pathology
- Horse Diseases / pathology
- Horses
- Immunoenzyme Techniques / veterinary
- In Situ Nick-End Labeling / veterinary
- Male
- Tendinopathy / metabolism
- Tendinopathy / pathology
- Tendinopathy / veterinary
- Tendon Injuries / pathology
- Tendon Injuries / veterinary
- Tendons / chemistry
- Tendons / metabolism
- Tendons / pathology
Citations
This article has been cited 6 times.- Brandt L, Schubert S, Scheibe P, Brehm W, Franzen J, Gross C, Burk J. Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment.. Int J Mol Sci 2018 Aug 28;19(9).
- Kondratko-Mittnacht J, Lakes R, Vanderby R Jr. Shear loads induce cellular damage in tendon fascicles.. J Biomech 2015 Sep 18;48(12):3299-305.
- Egerbacher M, Arnoczky SP, Caballero O, Lavagnino M, Gardner KL. Loss of homeostatic tension induces apoptosis in tendon cells: an in vitro study.. Clin Orthop Relat Res 2008 Jul;466(7):1562-8.
- Scott A, Alfredson H, Forsgren S. VGluT2 expression in painful Achilles and patellar tendinosis: evidence of local glutamate release by tenocytes.. J Orthop Res 2008 May;26(5):685-92.
- Stanley RL, Fleck RA, Becker DL, Goodship AE, Ralphs JR, Patterson-Kane JC. Gap junction protein expression and cellularity: comparison of immature and adult equine digital tendons.. J Anat 2007 Sep;211(3):325-34.
- Arnoczky SP, Lavagnino M, Egerbacher M. The mechanobiological aetiopathogenesis of tendinopathy: is it the over-stimulation or the under-stimulation of tendon cells?. Int J Exp Pathol 2007 Aug;88(4):217-26.
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