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Drug testing and analysis2022; 14(5); 794-807; doi: 10.1002/dta.3245

Metabolomics in clinical and forensic toxicology, sports anti-doping and veterinary residues.

Abstract: Metabolomics is a multidisciplinary field providing workflows for complementary approaches to conventional analytical determinations. It allows for the study of metabolically related groups of compounds or even the study of novel pathways within the biological system. The procedural stages of metabolomics; experimental design, sample preparation, analytical determinations, data processing and statistical analysis, compound identification and validation strategies are explored in this review. The selected approach will depend on the type of study being conducted. Experimental design influences the whole metabolomics workflow and thus needs to be properly assessed to ensure sufficient sample size, minimal introduced and biological variation and appropriate statistical power. Sample preparation needs to be simple, yet potentially global in order to detect as many compounds as possible. Analytical determinations need to be optimised either for the list of targeted compounds or a universal approach. Data processing and statistical analysis approaches vary widely and need to be better harmonised for review and interpretation. This includes validation strategies that are currently deficient in many presented workflows. Common compound identification approaches have been explored in this review. Metabolomics applications are discussed for clinical and forensic toxicology, human and equine sports anti-doping and veterinary residues.
© 2022 The Authors. Drug Testing and Analysis published by John Wiley & Sons Ltd.
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Publication Date: 2022-03-08 PubMed ID: 35194967PubMed Central: PMC9544538DOI: 10.1002/dta.3245Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper provides an in-depth examination of the procedural stages in metabolomics, which is a multidisciplinary field used as a supplementary approach to customary analytical findings. It identifies the potential application of metabolomics in areas like clinical and forensic toxicology, sports anti-doping, and veterinary residues.

Metabolomics: An Overview

  • Metabolomics is a multidisciplinary field that involves the detailed study of metabolic groups or compounds, and the identification of new pathways within biological systems. It works as a complementary methodology to routine analytical determinations.

Procedural Stages in Metabolomics

  • The research paper delves into various procedural stages of metabolomics which include experimental design, sample preparation, analytical determinations, data processing and statistical analysis, compound identification and validation strategies.
  • The choice of procedure hugely depends on the type of study being conducted.

Experimental Design

  • A well-thought-out experimental design impacts the whole metabolomics workflow. It should consider factors such as ensuring a sufficient sample size, minimizing introduced and biological variation, and achieving acceptable statistical power.

Sample Preparation

  • The sample preparation in metabolomics needs to be straightforward while trying to detect as many compounds as possible, which means it needs to be potentially global.

Analytical Determinations

  • These need optimization either for the list of targeted compounds or for a more generalized approach. The methods vary widely across studies.

Data Processing and Statistical Analysis

  • Approaches for data processing and statistical analysis often differ widely and need harmonization for easier review and interpretation.

Validation Strategies

  • These are typically poor in most presented workflows, indicating a significant gap which needs addressing.

Compound Identification

  • The paper explores different approaches to compound identification used in metabolomics.

Applications of Metabolomics

  • The paper gives an overview of the potential application of metabolomics in several sectors such as clinical and forensic toxicology, human and equine sports anti-doping, and the identification of veterinary residues.

Cite This Article

APA
Keen B, Cawley A, Reedy B, Fu S. (2022). Metabolomics in clinical and forensic toxicology, sports anti-doping and veterinary residues. Drug Test Anal, 14(5), 794-807. https://doi.org/10.1002/dta.3245

Publication

Drug testing and analysis
ISSN: 1942-7611
NlmUniqueID: 101483449
Country: England
Language: English
Volume: 14
Issue: 5
Pages: 794-807

Researcher Affiliations

Keen, Bethany
  • Centre for Forensic Science, University of Technology Sydney, Broadway, New South Wales, Australia.
Cawley, Adam
  • Australian Racing Forensic Laboratory, Racing NSW, Sydney, New South Wales, Australia.
Reedy, Brian
  • School of Mathematical and Physical Sciences, University of Technology Sydney, Broadway, New South Wales, Australia.
Fu, Shanlin
  • Centre for Forensic Science, University of Technology Sydney, Broadway, New South Wales, Australia.

MeSH Terms

  • Animals
  • Doping in Sports
  • Forensic Toxicology
  • Horses
  • Metabolomics
  • Sports
  • Workflow

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