Method for quantifying net anaerobic power in exercising horses.
Abstract: There is no good method for measuring net anaerobic power in exercising horses to allow accurate estimates of total metabolic power. Objective: The increase in VO2max when breathing hyperoxic (HO) gas should be accompanied by a stoichiometrically equal (in terms of ATP turnover, i.e. energy equivalents) decrease in plasma lactate accumulation rate (Mlactate). Methods: Six 3-year-old Thoroughbreds were trained on an equine treadmill wearing a semi-open flow mask for measurement of VO2. After 4 months the horses ran with reproducible specific VO2max (VO2max/kg bwt). The mask design allowed mixing of O2 or N2 with the inward bias flow of gas so that inspired O2 concentration of the horse could be controlled. While the horse breathed either HO (25.1% O2), normoxic (NO, 21% O2) or hypoxic (LO, 19.5% O2) gas, it ran at a speed sufficient to elicit VO2max in NO while jugular venous blood was drawn at 15 sec intervals over a period of 2 min to determine Mlactate. Results: VO2max/kg bwt was not significantly different between LO and NO conditions, and LO data could not be used in the comparison. The VO2max/kg bwt increased from 2.59 +/- 0.24 (s.d.) to 2.86 +/- 0.24 mlO2 (STPD)/sec/kg in NO and HO, respectively, while Mlactate decreased from 11.5 +/- 4.2 to 9.0 +/- 3.9 mmol/min as VO2 increased. Conclusions: The ratio of delta Mlactate to delta VO2max/kg bwt suggests that Mlactate of approx 11.1 +/- 6.7 mmol/min is associated with net anaerobic power approximately equivalent to 1.0 mlO2 (STPD)/sec/kg of aerobic power (20.1 W/kg(-1)). The high variability in VO2max/kg bwt observed in data from some runs, particularly in LO, suggests that caution must be used when comparing data from the same horse during different runs. Conclusions: This study provides a tool for estimating net anaerobic power and, more accurately, evaluating total metabolic power of horses exercising at or above their aerobic capacities.
Publication Date: 2007-04-04 PubMed ID: 17402450DOI: 10.1111/j.2042-3306.2006.tb05571.xGoogle Scholar: Lookup
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- Journal Article
- Randomized Controlled Trial
Summary
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The research article describes a method for measuring net anaerobic power in exercising horses, which can contribute to a more accurate evaluation of the animals’ total metabolic power while they’re exercising at or above their aerobic capacities.
Introduction
- The authors state that there’s a lack of efficient methods for measuring net anaerobic power in exercising horses; such tools can help generate accurate estimates of total metabolic power. They aimed to achieve this by analyzing the increase in maximum oxygen consumption (VO2max) when a horse breathes hyperoxic (HO) gas, expecting a decrease in the rate of plasma lactate accumulation (Mlactate).
Methodology
- Six 3-year-old Thoroughbreds were trained on a treadmill while wearing a semi-open flow mask to measure VO2. After four months of training, the horses were able to run with a reproducible specific VO2max.
- The mask was designed to allow the mixing of oxygen or nitrogen with the inward bias flow of gas, giving researchers control over the oxygen concentration that the horse inhaled. The horses ran at a speed that provoked VO2max in normal oxygen (NO) conditions while their blood was drawn every 15 seconds over 2 minutes to determine Mlactate levels.
- The horses performed this exercise while breathing through three different gas conditions; hyperoxic (higher oxygen concentration), normoxic (normal oxygen concentration), and hypoxic (lower oxygen concentration).
Results
- The researchers found that VO2max per body weight was not significantly different in LO and NO conditions, thus the data from these tests were not used in the comparison.
- In NO and HO conditions, VO2max increased while Mlactate decreased, suggesting that a higher oxygen concentration led to a decrease in lactate production.
Conclusions
- The study found a relationship between changes in Mlactate levels and changes in VO2max per body weight, suggesting a certain amount of Mlactate is associated with net anaerobic power equivalent to a specific measure of aerobic power.
- The authors noted a high variability in VO2max per body weight in data from some runs (especially in lower oxygen concentration), which led them to conclude that caution must be exercised when comparing data from the same horse during different runs.
- Ultimately, the study provides a useful method for estimating net anaerobic power and evaluating total metabolic power of horses exercising at or above their aerobic capacities.
Cite This Article
APA
Ohmura H, Hiraga A, Jones JH.
(2007).
Method for quantifying net anaerobic power in exercising horses.
Equine Vet J Suppl(36), 370-373.
https://doi.org/10.1111/j.2042-3306.2006.tb05571.x Publication
Researcher Affiliations
- Department of Surgical and Radiological Sciences, School of Veterinary Medicine, University of California, Davis, CA 95616, USA.
MeSH Terms
- Anaerobiosis / physiology
- Animals
- Cross-Over Studies
- Energy Metabolism / physiology
- Female
- Horses / metabolism
- Lactates / blood
- Lactates / metabolism
- Male
- Oxygen / metabolism
- Oxygen / physiology
- Oxygen Consumption / physiology
- Physical Conditioning, Animal / physiology
Citations
This article has been cited 4 times.- Ohmura H, Mukai K, Takahashi Y, Takahashi T. Metabolomic analysis of skeletal muscle before and after strenuous exercise to fatigue. Sci Rep 2021 May 27;11(1):11261.
- Ohmura H, Mukai K, Matsui A, Takahashi T, Jones JH. Cardiopulmonary function during supramaximal exercise in hypoxia, normoxia and hyperoxia in Thoroughbred horses. J Equine Sci 2020;31(4):67-73.
- Birks EK, Ohmura H, Jones JH. Measuring V̇O(2) in hypoxic and hyperoxic conditions using dynamic gas mixing with a flow-through indirect calorimeter. J Equine Sci 2019;30(4):87-92.
- Ohmura H, Mukai K, Takahashi Y, Takahashi T, Jones JH. Hypoxic training increases maximal oxygen consumption in Thoroughbred horses well-trained in normoxia. J Equine Sci 2017;28(2):41-45.
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