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Home / Equine Research Bank / Immunogenetics / MHC haplotype diversity in Persian Arabian horses determined...
Immunogenetics2017; 70(5); 305-315; doi: 10.1007/s00251-017-1039-x

MHC haplotype diversity in Persian Arabian horses determined using polymorphic microsatellites.

Abstract: Previous research on the equine major histocompatibility complex (MHC) demonstrated strong correlations between haplotypes defined by polymorphic intra-MHC microsatellites and haplotypes defined using classical serology. Here, we estimated MHC diversity in a sample of 124 Arabian horses from an endangered strain native to Iran (Persian Asil Arabians), using a validated 10-marker microsatellite panel. In a group of 66 horses related as parent-offspring pairs or half-sibling groups, we defined 51 MHC haplotypes, 49 of which were new. In 47 of the remaining 58 unrelated horses, we could assign one previously identified MHC haplotype, and by default, we gave provisional haplotype status to the remaining constellation of microsatellite alleles. In these horses, we found 21 haplotypes that we had previously defined and 31 provisional haplotypes, two of which had been identified in an earlier study. This gave a total of 78 new MHC haplotypes. The final 11 horses were MHC heterozygotes that we could not phase using information from any of the previously validated or provisional haplotypes. However, we could determine that these horses carried a total of 22 different undefined haplotypes. In the overall population sample, we detected three homozygous horses and one maternally inherited recombinant from 21 informative segregations. Virtually all of the horses tested were MHC heterozygotes, and most unrelated horses (98%) were heterozygous for rare microsatellite-defined haplotypes found less than three times in the sampled horses. This is evidence for a very high level of MHC haplotype variation in the Persian Asil Arabian horse.
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Publication Date: 2017-11-23 PubMed ID: 29170799DOI: 10.1007/s00251-017-1039-xGoogle Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research studied genetic diversity in the major histocompatibility complex (MHC) of Persian Asil Arabian horses using a 10-marker microsatellite panel. The conducted study revealed exceptionally high MHC haplotype variation in this specific breed.

Understanding the Major Histocompatibility Complex (MHC)

  • MHC is a set of genes in animals that play a critical role in the immune response. They are responsible for recognizing foreign cells in the host’s body and marking them for destruction by the immune system.
  • The diversity of these genes, as it relates, to individuals of a species is used as an indicator of the species’ overall genetic diversity.

Microsatellites and Their Role in This Research

  • Microsatellites are a kind of genetic marker that consists of short sequences of DNA tandemly repeated.
  • The researchers made use of a validated 10-marker microsatllite panel to estimate the MHC diversity in Persian Asil Arabian horses.
  • These microsatellites lies within the MHC itself and are helpful in establishing MHC haplotypes.

Explanation of the Findings

  • The research was carried out on 124 Arabian horses of a native endangered strain from Iran. Of these, 66 were part of parent-offspring pairs or half-sibling groups from which 51 MHC haplotypes (unique combinations of alleles) were defined. Interestingly, 49 of these haplotypes were new and previously unidentified ones.
  • In the other 58 unrelated horses, 47 could be assigned one previously recognized MHC haplotype, while the remaining had provisional haplotype status with 31 provisional haplotypes, some of which were identified in a previous study.
  • The undetermined MHC heterozygotes contained 22 different undefined MHC haplotypes, contributing to the overall haplotype count (78 new MHC haplotypes).
  • Further, three homozygous horses and one maternally inherited recombinant horse were also found in the population sample.
  • The majority of the horses (~98% of unrelated ones) were MHC heterozygotes and had rare microsatellite-defined haplotypes, found fewer than three times in the sampled population, indicating significant genetic diversity.

Conclusion

  • The research concluded that Persian Asil Arabian horses exhibit a high level of MHC haplotype variation. This high level of genetic variation is vital for the species’ ability to adapt to changing environments and resist diseases.

Cite This Article

APA
Sadeghi R, Moradi-Shahrbabak M, Miraei Ashtiani SR, Miller DC, Antczak DF. (2017). MHC haplotype diversity in Persian Arabian horses determined using polymorphic microsatellites. Immunogenetics, 70(5), 305-315. https://doi.org/10.1007/s00251-017-1039-x

Publication

Immunogenetics
ISSN: 1432-1211
NlmUniqueID: 0420404
Country: United States
Language: English
Volume: 70
Issue: 5
Pages: 305-315

Researcher Affiliations

Sadeghi, R
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
  • Department of Animal Science, University of Tehran, Karaj, 4111, Iran.
Moradi-Shahrbabak, Mohammad
  • Department of Animal Science, University of Tehran, Karaj, 4111, Iran. moradim@ut.ac.ir.
Miraei Ashtiani, S R
  • Department of Animal Science, University of Tehran, Karaj, 4111, Iran.
Miller, D C
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA.
Antczak, Douglas F
  • Baker Institute for Animal Health, College of Veterinary Medicine, Cornell University, Ithaca, NY, 14853, USA. dfa1@cornell.edu.

MeSH Terms

  • Animals
  • Female
  • Haplotypes
  • Horses / genetics
  • Horses / immunology
  • Major Histocompatibility Complex
  • Male
  • Microsatellite Repeats
  • Persia
  • Polymorphism, Genetic
  • Sequence Analysis, DNA

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