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Home / Equine Research Bank / Vet Microbiol / Microbiology of equine wounds and evidence of bacterial biof...
Veterinary microbiology2011; 150(1-2); 152-159; doi: 10.1016/j.vetmic.2011.01.003

Microbiology of equine wounds and evidence of bacterial biofilms.

Abstract: Horse wounds have a high risk of becoming infected due to their environment. Infected wounds harbour diverse populations of microorganisms, however in some cases these microorganisms can be difficult to identify and fail to respond to antibiotic treatment, resulting in chronic non-healing wounds. In human wounds this has been attributed to the ability of bacteria to survive in a biofilm phenotypic state. Biofilms are known to delay wound healing, principally due to their recalcitrance towards antimicrobial therapies and components of the innate immune response. This study describes the presence of bacterial biofilms within equine wounds. Thirteen 8-mm diameter tissue samples were collected from (n=18) chronic wounds. Following histological staining, samples were observed for evidence of biofilms. Fifty one wounds and control skin sites were sampled using sterile swabs. Control skin sites were on the uninjured side of the horse at the same anatomical location as the wound. The isolated bacteria were cultured aerobically and anaerobically. The biofilm forming potential of all the isolated bacteria was determined using a standard crystal violet microtitre plate assay. Stained tissue samples provided evidence of biofilms within 61.5% (8 out of 13) equine wounds. In total 340 bacterial isolates were identified from all the equine wound and skin samples. Pseudomonas aeruginosa and Enterococcus faecium were the most predominantly isolated bacterial species from equine wound and skin samples respectively. Staphylococcus was the most commonly isolated genus in both environments. Bacteria cultured from chronic and acute wounds showed significantly (P<0.05) higher biofilm forming potential than bacteria isolated from skin. This paper highlights preliminary evidence supporting the presence of biofilms and a high microbial diversity in equine chronic wounds. The presence of biofilms in equine wounds partly explains the reluctance of many lower limb wounds to heal. Non-healing limb wounds in horses are a well documented welfare and economic concern. This knowledge can be used to shape future treatments in order to increase the healing rate and decrease the costs and suffering associate with equine wounds.
Copyright © 2011 Elsevier B.V. All rights reserved.
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Publication Date: 2011-01-11 PubMed ID: 21273008DOI: 10.1016/j.vetmic.2011.01.003Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study examines the microbiology of horse wounds, with a particular focus on bacterial biofilms, types of microbial communities that are notoriously resistant to treatment. The study highlights the complex diversity of bacteria in these wounds, confirms the existence of biofilms, and infers that these biofilms likely explain why many equine wounds become chronic and struggle to heal.

Research Methodology

  • The researchers collected 13 8-mm wide tissue samples from chronic wounds on 18 horses. The diversity and structure of the bacterial communities in these samples were then studied.
  • A histological staining method was used to visually observe the samples for biofilms – structural colonies of bacteria that can lodge in tissue.
  • A further 51 wound and control skin sites were sampled with sterile swabs. Control sites were chosen as the uninjured regions on the opposite side of the horse as the wound, at a similar anatomical location.
  • The isolated bacteria were cultured both in the presence (aerobically) and absence (anaerobically) of oxygen, to provide further details on the nature and diversity of the bacterial populations.
  • The bacteria’s ability to form biofilms was determined with a standard assay test using crystal violet, a common staining agent.

Key Findings

  • Following the staining process, bacteria biofilms were seen to be present in 8 of the 13 (61.5%) equine wounds analysed.
  • The researchers identified 340 unique bacterial isolates from all the wound and skin samples studied. The most commonly isolated species were Pseudomonas aeruginosa from wound samples and Enterococcus faecium from the skin samples.
  • Alongside these, Staphylococcus was the most commonly isolated bacterial genus across both types of samples.
  • The study found that bacteria cultured from both the chronic and acute wounds had a significantly higher potential to form biofilms than bacteria from skin samples.

Significance of the Study

  • This study provides preliminary evidence of bacterial biofilms existing within chronic horse wounds, backing the hypothesis of their role in making these wounds resistant to healing.
  • It calculates a high level of microbial diversity in these wounds, detailing bacterial species that may be crucial in understanding the treatment (or lack thereof) of such wounds.
  • The research underlines the welfare and economic concern of non-healing limb wounds in horses, and the importance of understanding the underlying factors to inform future treatment strategies. The aim is to increase healing rates, decrease costs, and alleviate the suffering associated with persistent equine wounds.

Cite This Article

APA
Westgate SJ, Percival SL, Knottenbelt DC, Clegg PD, Cochrane CA. (2011). Microbiology of equine wounds and evidence of bacterial biofilms. Vet Microbiol, 150(1-2), 152-159. https://doi.org/10.1016/j.vetmic.2011.01.003

Publication

Veterinary microbiology
ISSN: 1873-2542
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 150
Issue: 1-2
Pages: 152-159

Researcher Affiliations

Westgate, S J
  • University of Liverpool, School of Veterinary Sciences, Faculty of Health and Life Sciences, Leahurst, Neston, South Wirral CH64 7TE, United Kingdom. s.j.westgate@liv.ac.uk
Percival, S L
    Knottenbelt, D C
      Clegg, P D
        Cochrane, C A

          MeSH Terms

          • Animals
          • Biofilms
          • Enterococcus faecium / isolation & purification
          • Enterococcus faecium / pathogenicity
          • Gram-Positive Bacterial Infections / microbiology
          • Horse Diseases / microbiology
          • Horses / microbiology
          • Pseudomonas Infections / microbiology
          • Pseudomonas Infections / pathology
          • Pseudomonas aeruginosa / isolation & purification
          • Pseudomonas aeruginosa / pathogenicity
          • Skin Diseases, Bacterial / microbiology
          • Staphylococcal Infections / microbiology
          • Staphylococcus / isolation & purification
          • Staphylococcus / pathogenicity
          • Wound Infection / microbiology

          Citations

          This article has been cited 33 times.
          1. Shouqair D, Alghafri R, Naji M, Albastaki A, Nassar R, Mohamed L, Aloba B, Awad BS, Al Dhaheri F, Everett D, Habib I, Almashadani M, Shibl AA, Rodríguez J, Moradigaravand D, Monecke S, Ehricht R, Khan M, Goering R, Senok A. Culture-Based Wastewater Surveillance for the Detection and Monitoring of Antimicrobial Resistance in Staphylococcal Species. Vet Sci 2025 Dec 23;13(1).
            doi: 10.3390/vetsci13010014pubmed: 41600670google scholar: lookup
          2. Wesołowska M, Szczuka E. Analysis of Staphylococcal Diversity in the Skin Microbiota of Healthy Riding Horses. Antibiotics (Basel) 2025 Oct 16;14(10).
            doi: 10.3390/antibiotics14101037pubmed: 41148728google scholar: lookup
          3. Köhne M, Hüsch R, Peh E, Hirnet J, Tönissen A, Müsken M, Plötz M, Kittler S, Sieme H. Newly isolated bacteriophages show efficacy and phage-antibiotic synergy in vitro against the equine genital pathogens Klebsiella pneumoniae and Pseudomonas aeruginosa. BMC Vet Res 2025 Oct 3;21(1):568.
            doi: 10.1186/s12917-025-04989-1pubmed: 41044673google scholar: lookup
          4. Beshir A, Kemal J, Abraha B, Tola EH. Isolation of major bacterial species associated with equine skin wounds and in-vitro antibacterial activities of selected medicinal plants. Sci Rep 2025 Aug 29;15(1):31942.
            doi: 10.1038/s41598-025-01062-7pubmed: 40883320google scholar: lookup
          5. Marcolina M, Williams ZJ, Hendrickson D, Pezzanite LM. Evaluation of Sterility of Saline Formulations Manufactured for Wound Care in Veterinary Practice. Vet Sci 2025 Apr 30;12(5).
            doi: 10.3390/vetsci12050431pubmed: 40431524google scholar: lookup
          6. Allano M, Arsenault J, Archambault M, Fairbrother JH, Sauvé F. Prevalence and Risk Factors of Staphylococcus aureus Nasal Colonization in Horses Admitted to a Veterinary Teaching Hospital. J Vet Intern Med 2025 May-Jun;39(3):e70027.
            doi: 10.1111/jvim.70027pubmed: 40135807google scholar: lookup
          7. Mayer P, Smith AC, Hurlow J, Morrow BR, Bohn GA, Bowler PG. Assessing Biofilm at the Bedside: Exploring Reliable Accessible Biofilm Detection Methods. Diagnostics (Basel) 2024 Sep 24;14(19).
            doi: 10.3390/diagnostics14192116pubmed: 39410520google scholar: lookup
          8. Ali HR, Collier P, Bayston R. A Three-Dimensional Model of Bacterial Biofilms and Its Use in Antimicrobial Susceptibility Testing. Microorganisms 2024 Jan 19;12(1).
            doi: 10.3390/microorganisms12010203pubmed: 38276189google scholar: lookup
          9. Pimenta J, Dias C, Cotovio M, Saavedra MJ. In Vitro Effect of Eucalyptus Essential Oils and Antiseptics (Chlorhexidine Gluconate and Povidone-Iodine) against Bacterial Isolates from Equine Wounds. Vet Sci 2023 Dec 26;11(1).
            doi: 10.3390/vetsci11010012pubmed: 38250918google scholar: lookup
          10. Labens R, Raidal S, Borgen-Nielsen C, Pyecroft S, Pant SD, De Ridder T. Wound healing of experimental equine skin wounds and concurrent microbiota in wound dressings following topical propylene glycol gel treatment. Front Vet Sci 2023;10:1294021.
            doi: 10.3389/fvets.2023.1294021pubmed: 38155761google scholar: lookup
          11. Afonso AC, Sousa M, Pinto AR, Cotovio M, Simões M, Saavedra MJ. Biofilm Production by Critical Antibiotic-Resistant Pathogens from an Equine Wound. Animals (Basel) 2023 Apr 13;13(8).
            doi: 10.3390/ani13081342pubmed: 37106905google scholar: lookup
          12. Nesse LL, Osland AM, Vestby LK. The Role of Biofilms in the Pathogenesis of Animal Bacterial Infections. Microorganisms 2023 Feb 28;11(3).
            doi: 10.3390/microorganisms11030608pubmed: 36985183google scholar: lookup
          13. Brock AK, Chamoun-Emanuelli AM, Howard EA, Huntzinger KD, Lawhon SD, Bryan LK, Cosgriff-Hernandez EM, Cohen ND, Whitfield-Cargile CM. Wound swabs versus biopsies to detect methicillin resistant Staphylococcus aureus in experimental equine wounds. Vet Surg 2022 Nov;51(8):1196-1205.
            doi: 10.1111/vsu.13872pubmed: 36102600google scholar: lookup
          14. Jørgensen E, Bjarnsholt T, Jacobsen S. Biofilm and Equine Limb Wounds. Animals (Basel) 2021 Sep 27;11(10).
            doi: 10.3390/ani11102825pubmed: 34679846google scholar: lookup
          15. Marx C, Gardner S, Harman RM, Wagner B, Van de Walle GR. Mesenchymal stromal cell-secreted CCL2 promotes antibacterial defense mechanisms through increased antimicrobial peptide expression in keratinocytes. Stem Cells Transl Med 2021 Dec;10(12):1666-1679.
            doi: 10.1002/sctm.21-0058pubmed: 34528765google scholar: lookup
          16. Little SV, Hillhouse AE, Lawhon SD, Bryan LK. Analysis of Virulence and Antimicrobial Resistance Gene Carriage in Staphylococcus aureus Infections in Equids Using Whole-Genome Sequencing. mSphere 2021 Aug 25;6(4):e0019620.
            doi: 10.1128/mSphere.00196-20pubmed: 34346711google scholar: lookup
          17. Rahman AZA, Adzahan NM, Zakaria Z, Mayaki AM. Antibacterial effect of acidic ionized water on horse wounds bacterial isolates. Vet World 2021 May;14(5):1128-1132.
            doi: 10.14202/vetworld.2021.1128-1132pubmed: 34220113google scholar: lookup
          18. El-Deeb NM, Abo-Eleneen MA, Al-Madboly LA, Sharaf MM, Othman SS, Ibrahim OM, Mubarak MS. Biogenically Synthesized Polysaccharides-Capped Silver Nanoparticles: Immunomodulatory and Antibacterial Potentialities Against Resistant Pseudomonas aeruginosa. Front Bioeng Biotechnol 2020;8:643.
            doi: 10.3389/fbioe.2020.00643pubmed: 32793561google scholar: lookup
          19. Wilmink JM, Ladefoged S, Jongbloets A, Vernooij JCM. The evaluation of the effect of probiotics on the healing of equine distal limb wounds. PLoS One 2020;15(7):e0236761.
            doi: 10.1371/journal.pone.0236761pubmed: 32726347google scholar: lookup
          20. Lawless SP, Cohen ND, Lawhon SD, Chamoun-Emanuelli AM, Wu J, Rivera-Vélez A, Weeks BR, Whitfield-Cargile CM. Effect of gallium maltolate on a model of chronic, infected equine distal limb wounds. PLoS One 2020;15(6):e0235006.
            doi: 10.1371/journal.pone.0235006pubmed: 32559258google scholar: lookup
          21. Marx C, Gardner S, Harman RM, Van de Walle GR. The mesenchymal stromal cell secretome impairs methicillin-resistant Staphylococcus aureus biofilms via cysteine protease activity in the equine model. Stem Cells Transl Med 2020 Jul;9(7):746-757.
            doi: 10.1002/sctm.19-0333pubmed: 32216094google scholar: lookup
          22. Jørgensen E, Bay L, Skovgaard LT, Bjarnsholt T, Jacobsen S. An Equine Wound Model to Study Effects of Bacterial Aggregates on Wound Healing. Adv Wound Care (New Rochelle) 2019 Oct 1;8(10):487-498.
            doi: 10.1089/wound.2018.0901pubmed: 31456906google scholar: lookup
          23. Kamus LJ, Theoret C, Costa MC. Use of next generation sequencing to investigate the microbiota of experimentally induced wounds and the effect of bandaging in horses. PLoS One 2018;13(11):e0206989.
            doi: 10.1371/journal.pone.0206989pubmed: 30475922google scholar: lookup
          24. Ferris RA, McCue PM, Borlee GI, Glapa KE, Martin KH, Mangalea MR, Hennet ML, Wolfe LM, Broeckling CD, Borlee BR. Model of Chronic Equine Endometritis Involving a Pseudomonas aeruginosa Biofilm. Infect Immun 2017 Dec;85(12).
            doi: 10.1128/IAI.00332-17pubmed: 28970274google scholar: lookup
          25. Harman RM, Yang S, He MK, Van de Walle GR. Antimicrobial peptides secreted by equine mesenchymal stromal cells inhibit the growth of bacteria commonly found in skin wounds. Stem Cell Res Ther 2017 Jul 4;8(1):157.
            doi: 10.1186/s13287-017-0610-6pubmed: 28676123google scholar: lookup
          26. Olofsson TC, Butler É, Lindholm C, Nilson B, Michanek P, Vásquez A. Fighting Off Wound Pathogens in Horses with Honeybee Lactic Acid Bacteria. Curr Microbiol 2016 Oct;73(4):463-73.
            doi: 10.1007/s00284-016-1080-2pubmed: 27324340google scholar: lookup
          27. Nour El Din S, El-Tayeb TA, Abou-Aisha K, El-Azizi M. In vitro and in vivo antimicrobial activity of combined therapy of silver nanoparticles and visible blue light against Pseudomonas aeruginosa. Int J Nanomedicine 2016;11:1749-58.
            doi: 10.2147/IJN.S102398pubmed: 27175075google scholar: lookup
          28. Drzewiecka D. Significance and Roles of Proteus spp. Bacteria in Natural Environments. Microb Ecol 2016 Nov;72(4):741-758.
            doi: 10.1007/s00248-015-0720-6pubmed: 26748500google scholar: lookup
          29. Ferris RA, McCue PM, Borlee GI, Loncar KD, Hennet ML, Borlee BR. In Vitro Efficacy of Nonantibiotic Treatments on Biofilm Disruption of Gram-Negative Pathogens and an In Vivo Model of Infectious Endometritis Utilizing Isolates from the Equine Uterus. J Clin Microbiol 2016 Mar;54(3):631-9.
            doi: 10.1128/JCM.02861-15pubmed: 26719448google scholar: lookup
          30. Jones EM, Cochrane CA, Percival SL. The Effect of pH on the Extracellular Matrix and Biofilms. Adv Wound Care (New Rochelle) 2015 Jul 1;4(7):431-439.
            doi: 10.1089/wound.2014.0538pubmed: 26155386google scholar: lookup
          31. Percival SL, Vuotto C, Donelli G, Lipsky BA. Biofilms and Wounds: An Identification Algorithm and Potential Treatment Options. Adv Wound Care (New Rochelle) 2015 Jul 1;4(7):389-397.
            doi: 10.1089/wound.2014.0574pubmed: 26155381google scholar: lookup
          32. Percival SL, McCarty SM, Lipsky B. Biofilms and Wounds: An Overview of the Evidence. Adv Wound Care (New Rochelle) 2015 Jul 1;4(7):373-381.
            doi: 10.1089/wound.2014.0557pubmed: 26155379google scholar: lookup
          33. Percival SL, Emanuel C, Cutting KF, Williams DW. Microbiology of the skin and the role of biofilms in infection. Int Wound J 2012 Feb;9(1):14-32.
            doi: 10.1111/j.1742-481X.2011.00836.xpubmed: 21973162google scholar: lookup
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