Microvascular permeability changes in ischemia/reperfusion injury in the ascending colon of horses.
Abstract: The normal microvascular permeability of the ascending colon in horses and the microvascular permeability of that segment after ischemia and reperfusion were investigated. Microvascular permeability was estimated by the ratio of lymphatic protein to plasma protein concentration (Cl/Cp) at high lymph flow rates in 8 adult horses in 2 equal groups: normal and ischemic (2-hour period). Lymphatic flow rates and lymph and plasma protein concentrations were determined. Intestinal biopsy specimens were obtained at the end of each experiment. Flow independent values were selected and compared by one-way ANOVA, and the mean and SEM of these values were determined. The mean Cl/Cp ratios for the flow independent part of each data set were as follows: normal = 0.36 +/- 0.08; ischemic = 0.70 +/- 0.08. These groups were significantly different (P < or = 0.0001). Microscopic evaluation revealed mild congestion and edema in the normal group. The ischemic group had mild to moderate mucosal degeneration, with moderate to severe congestion and edema. We concluded that ischemia of the ascending colon, when followed by reperfusion, results in a significant increase in microvascular permeability.
Publication Date: 1992-10-15 PubMed ID: 1429157
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research article explores the significant increase in microvascular permeability (the ability of small blood vessels to allow substances to pass through) of the ascending colon in horses, when it undergoes ischemia and reperfusion (blockage and restoration of blood flow).
Objective of the Research
- The article investigates the normal microvascular permeability of the ascending colon in horses as well as the changes in microvascular permeability following ischemia and reperfusion of the same region.
Methodology
- The study involved 8 adult horses divided into two equal groups: normal and ischemic. The ischemic group went through a 2-hour ischemic period.
- The measure for microvascular permeability was the ratio of lymphatic protein to plasma protein concentration (Cl/Cp).
- Lymphatic flow rates, and lymph and plasma protein concentrations were evaluated.
- Intestinal biopsy specimens were obtained after each experiment for microscopic evaluation.
- Data with flow independent values were selected and subjected to one-way ANOVA for statistical analysis
Results and Conclusion
- The mean Cl/Cp ratios were as follows: for the normal group it was 0.36 +/- 0.08, while for the ischemic group it was 0.70 +/- 0.08 – a statistically significant difference emphasised by a P value less than 0.0001.
- The normal group showed only mild congestion and edema.
- The ischemic group, on the other hand, displayed mild to moderate mucosal degeneration coupled with moderate to severe congestion and edema.
- The researchers concluded that ischemia of the ascending colon, followed by reperfusion, leads to a significant increase in microvascular permeability. Meaning the tissue becomes more permeable, potentially allowing harmful substances to penetrate and cause damage.
Cite This Article
APA
Henninger DD, Snyder JR, Pascoe JR, Dilling GW.
(1992).
Microvascular permeability changes in ischemia/reperfusion injury in the ascending colon of horses.
J Am Vet Med Assoc, 201(8), 1191-1196.
Publication
Researcher Affiliations
- Veterinary Medical Teaching Hospital, Department of Surgery, School of Veterinary Medicine, University of California, Davis 95616.
MeSH Terms
- Animals
- Blood Proteins / analysis
- Capillary Permeability
- Colon / blood supply
- Horse Diseases / physiopathology
- Horses
- Lymph / chemistry
- Lymphatic System / physiopathology
- Proteins / analysis
- Reperfusion Injury / physiopathology
- Reperfusion Injury / veterinary
- Venous Pressure
Citations
This article has been cited 4 times.- Faleiros RR, Macoris DG, Alves GE, Souza DG, Teixeira MM, Moore RM. Local and remote lesions in horses subjected to small colon distension and decompression. Can J Vet Res 2008 Jan;72(1):68-76.
- Sharifi K, Mostaghni K, Maleki M, Badiei K. Ischaemia/reperfusion injury in experimentally induced abomasal volvulus in sheep. Vet Res Commun 2007 Jul;31(5):575-90.
- Moore RM, Sedrish SA, Holmes EP, Koch CE, Venugopal CS. Role of endothelium and nitric oxide in modulating in vitro responses of colonic arterial and venous rings to vasodilatory neuropeptides in horses. Can J Vet Res 2005 Apr;69(2):116-22.
- Moore RM, Muir WW, Rush BR. Systemic and colonic venous plasma biochemical alterations in horses during low-flow ischemia and reperfusion of the large colon. Can J Vet Res 1998 Jan;62(1):14-20.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
