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Animals : an open access journal from MDPI2020; 10(5); 880; doi: 10.3390/ani10050880

Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid.

Abstract: Sarcoids are the most common skin neoplasm in the Equidae family. Sarcoids are benign, but may cause severe damage in affected animals. Due to the high risk of post-treatment recurrence and the lack of an effective method of treatment, it is reasonable to perform studies on the molecular aspects of this neoplasm. Therefore, the present studies analyzed five genes (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) related to cell mobility and invasion traits. Primary healthy fibroblasts and sarcoid cells were obtained from skin biopsies. Cell lines were cultured in two different medium types with different concentrations of foetal bovine serum (10% and 0.5% FBS) to study its influence on the analyzed genes. Gene expression was measured using the real-time PCR method. The results showed significant differences in two genes (coronin and vimentin) depending on culture conditions. In conclusion, the results enabled finding two new genes, related to cell motility and invasion traits, in which gene expression is deregulated. Results of the study may put new knowledge into the complexity of the genetic background of this disease and show the importance of further analysis on this subject.
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Publication Date: 2020-05-19 PubMed ID: 32438542PubMed Central: PMC7278424DOI: 10.3390/ani10050880Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the differences in gene expression related to cell mobility and invasion traits in equine sarcoids, a common skin neoplasm in horses. It sheds light on the complexities of the genetic background of this disease and identifies two genes where there’s unusual gene expression.

Research Objectives and Methodology

The intent of this research was to gain a deeper understanding of equine sarcoids, the most common skin growth found in the Equidae family. This disease, while benign, can cause significant harm to affected animals. Of particular concern is the high risk of recurrence post-treatment and the lack of a fully effective treatment method. These issues justify pursuing research into the molecular aspects of the disease.

  • The researchers focused on the analysis of five specific genes that relate to cell mobility and invasion traits to investigate their possible role in the development of equine sarcoids.
  • The genes under examination were: cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3, and vimentin.
  • The study utilized primary healthy fibroblasts and sarcoid cells obtained from skin biopsies for the experiments.
  • The cell lines were cultivated in two differing mediums, each with a different concentration of fetal bovine serum (10% and 0.5% FBS), with the intention of determining the influence of this substance on the genes.

Findings and Conclusions

The results of the study revealed noticeable differences in the expression of two genes (coronin and vimentin) contingent on the culture conditions. This discovery hints that these genes may play a role in equine sarcoid formation.

  • It is suggested that the changes in gene expression are possibly related to the characteristics of cell motility and invasion, these being key features in the development and progression of cancers.
  • This research adds to the body of knowledge regarding the genetic background of the equine sarcoid disease and emphasizes the need for continued analysis. Having this knowledge could lead to more effective treatments for equine sarcoids in the future.

Cite This Article

APA
Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. (2020). Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals (Basel), 10(5), 880. https://doi.org/10.3390/ani10050880

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 5
PII: 880

Researcher Affiliations

Podstawski, Przemysław
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland.
Witarski, Wojciech
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Szmatoła, Tomasz
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
  • University Centre of Veterinary Medicine, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland.
Bugno-Poniewierska, Monika
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland.
Ropka-Molik, Katarzyna
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.

Grant Funding

  • 297267 / Narodowe Centrum Badau0144 i Rozwoju

Conflict of Interest Statement

The authors declare no conflicts of interest. The funders had no role in the design of the study, the collection, analyses, or interpretation of data, the writing of the manuscript, or the decision to publish the results.

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Citations

This article has been cited 1 times.
  1. Podstawski P, Ropka-Molik K, Semik-Gurgul E, Samiec M, Skrzyszowska M, Podstawski Z, Szmatoła T, Witkowski M, Pawlina-Tyszko K. Assessment of BPV-1 Mediated Matrix Metalloproteinase Genes Deregulation in the In Vivo and In Vitro Models Designed to Explore Molecular Nature of Equine Sarcoids. Cells 2022 Apr 8;11(8).
    doi: 10.3390/cells11081268pubmed: 35455948google scholar: lookup
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