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Animals : an open access journal from MDPI2020; 10(5); 880; doi: 10.3390/ani10050880

Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid.

Abstract: Sarcoids are the most common skin neoplasm in the Equidae family. Sarcoids are benign, but may cause severe damage in affected animals. Due to the high risk of post-treatment recurrence and the lack of an effective method of treatment, it is reasonable to perform studies on the molecular aspects of this neoplasm. Therefore, the present studies analyzed five genes (cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3 and vimentin) related to cell mobility and invasion traits. Primary healthy fibroblasts and sarcoid cells were obtained from skin biopsies. Cell lines were cultured in two different medium types with different concentrations of foetal bovine serum (10% and 0.5% FBS) to study its influence on the analyzed genes. Gene expression was measured using the real-time PCR method. The results showed significant differences in two genes (coronin and vimentin) depending on culture conditions. In conclusion, the results enabled finding two new genes, related to cell motility and invasion traits, in which gene expression is deregulated. Results of the study may put new knowledge into the complexity of the genetic background of this disease and show the importance of further analysis on this subject.
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Publication Date: 2020-05-19 PubMed ID: 32438542PubMed Central: PMC7278424DOI: 10.3390/ani10050880Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates the differences in gene expression related to cell mobility and invasion traits in equine sarcoids, a common skin neoplasm in horses. It sheds light on the complexities of the genetic background of this disease and identifies two genes where there’s unusual gene expression.

Research Objectives and Methodology

The intent of this research was to gain a deeper understanding of equine sarcoids, the most common skin growth found in the Equidae family. This disease, while benign, can cause significant harm to affected animals. Of particular concern is the high risk of recurrence post-treatment and the lack of a fully effective treatment method. These issues justify pursuing research into the molecular aspects of the disease.

  • The researchers focused on the analysis of five specific genes that relate to cell mobility and invasion traits to investigate their possible role in the development of equine sarcoids.
  • The genes under examination were: cell cycle control binding protein alpha, coronin 1b, metalloproteinase 2, tissue inhibitor of metalloproteinases 3, and vimentin.
  • The study utilized primary healthy fibroblasts and sarcoid cells obtained from skin biopsies for the experiments.
  • The cell lines were cultivated in two differing mediums, each with a different concentration of fetal bovine serum (10% and 0.5% FBS), with the intention of determining the influence of this substance on the genes.

Findings and Conclusions

The results of the study revealed noticeable differences in the expression of two genes (coronin and vimentin) contingent on the culture conditions. This discovery hints that these genes may play a role in equine sarcoid formation.

  • It is suggested that the changes in gene expression are possibly related to the characteristics of cell motility and invasion, these being key features in the development and progression of cancers.
  • This research adds to the body of knowledge regarding the genetic background of the equine sarcoid disease and emphasizes the need for continued analysis. Having this knowledge could lead to more effective treatments for equine sarcoids in the future.

Cite This Article

APA
Podstawski P, Witarski W, Szmatoła T, Bugno-Poniewierska M, Ropka-Molik K. (2020). Mobility and Invasion Related Gene Expression Patterns in Equine Sarcoid. Animals (Basel), 10(5), 880. https://doi.org/10.3390/ani10050880

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 10
Issue: 5
PII: 880

Researcher Affiliations

Podstawski, Przemysław
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland.
Witarski, Wojciech
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
Szmatoła, Tomasz
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.
  • University Centre of Veterinary Medicine, University of Agriculture in Kraków, Mickiewicza 24/28, 30-059 Kraków, Poland.
Bugno-Poniewierska, Monika
  • Department of Animal Reproduction, Anatomy and Genomics, University of Agriculture in Kraków, Mickiewicza24/28, 30-059 Kraków, Poland.
Ropka-Molik, Katarzyna
  • Department of Animal Molecular Biology, Laboratory of Genomics, National Research Institute of Animal Production, Krakowska 1, 32-083 Balice, Poland.

Grant Funding

  • 297267 / Narodowe Centrum Badau0144 i Rozwoju

Conflict of Interest Statement

The authors declare no conflicts of interest. The funders had no role in the design of the study, the collection, analyses, or interpretation of data, the writing of the manuscript, or the decision to publish the results.

References

This article includes 38 references
  1. Knottenbelt DC. A Suggested Clinical Classification for the Equine Sarcoid. Clin. Tech. Equine Pr. 2005;4:278–295.
    doi: 10.1053/j.ctep.2005.10.008google scholar: lookup
  2. Haralambus R, Burgstaller J, Klukowska-Rötzler J, Steinborn R, Buchinger S, Gerber V, Brandt S. Intralesional bovine papillomavirus DNA loads reflect severity of equine sarcoid disease.. Equine Vet J 2010 May;42(4):327-31.
    doi: 10.1111/j.2042-3306.2010.00078.xpubmed: 20525051google scholar: lookup
  3. Christen G, Gerber V, Dolf G, Burger D, Koch C. Inheritance of equine sarcoid disease in Franches-Montagnes horses.. Vet J 2014 Jan;199(1):68-71.
    doi: 10.1016/j.tvjl.2013.09.053pubmed: 24152383google scholar: lookup
  4. Knottenbelt DC, Matthews JB. A positive step forwards in the diagnosis of equine sarcoid.. Vet J 2001 May;161(3):224-6.
    doi: 10.1053/tvjl.2000.0555pubmed: 11352479google scholar: lookup
  5. Taylor SD, Toth B, Baseler LJ, Charney VA, Miller MA. Lack of Correlation Between Papillomaviral DNA in Surgical Margins and Recurrence of Equine Sarcoids. J. Equine Veter-Sci. 2014;34:722–725.
    doi: 10.1016/j.jevs.2013.12.012google scholar: lookup
  6. Nasir L, Reid SW. Bovine papillomaviral gene expression in equine sarcoid tumours.. Virus Res 1999 Jun;61(2):171-5.
    doi: 10.1016/S0168-1702(99)00022-2pubmed: 10475087google scholar: lookup
  7. Yuan Z, Gallagher A, Gault EA, Campo MS, Nasir L. Bovine papillomavirus infection in equine sarcoids and in bovine bladder cancers.. Vet J 2007 Nov;174(3):599-604.
    doi: 10.1016/j.tvjl.2006.10.012pubmed: 17150387google scholar: lookup
  8. Yuan ZQ, Gault EA, Gobeil P, Nixon C, Campo MS, Nasir L. Establishment and characterization of equine fibroblast cell lines transformed in vivo and in vitro by BPV-1: model systems for equine sarcoids.. Virology 2008 Apr 10;373(2):352-61.
    doi: 10.1016/j.virol.2007.11.037pubmed: 18191170google scholar: lookup
  9. Bogaert L, Martens A, Kast WM, Van Marck E, De Cock H. Bovine papillomavirus DNA can be detected in keratinocytes of equine sarcoid tumors.. Vet Microbiol 2010 Dec 15;146(3-4):269-75.
    doi: 10.1016/j.vetmic.2010.05.032pubmed: 21095508google scholar: lookup
  10. Gaynor AM, Zhu KW, Dela Cruz FN Jr, Affolter VK, Pesavento PA. Localization of Bovine Papillomavirus Nucleic Acid in Equine Sarcoids.. Vet Pathol 2016 May;53(3):567-73.
    doi: 10.1177/0300985815594852pubmed: 26215759google scholar: lookup
  11. Rector A, Van Ranst M. Animal papillomaviruses.. Virology 2013 Oct;445(1-2):213-23.
    doi: 10.1016/j.virol.2013.05.007pubmed: 23711385google scholar: lookup
  12. Bravo IG, Félez-Sánchez M. Papillomaviruses: Viral evolution, cancer and evolutionary medicine.. Evol Med Public Health 2015 Jan 28;2015(1):32-51.
    doi: 10.1093/emph/eov003pmc: PMC4356112pubmed: 25634317google scholar: lookup
  13. Semik E, Gurgul A, Ząbek T, Ropka-Molik K, Koch C, Mählmann K, Bugno-Poniewierska M. Transcriptome analysis of equine sarcoids.. Vet Comp Oncol 2017 Dec;15(4):1370-1381.
    doi: 10.1111/vco.12279pubmed: 27779365google scholar: lookup
  14. Pawlina K, Gurgul A, Szmatoła T, Koch C, Mählmann K, Witkowski M, Bugno-Poniewierska M. Comprehensive characteristics of microRNA expression profile of equine sarcoids.. Biochimie 2017 Jun;137:20-28.
    doi: 10.1016/j.biochi.2017.02.017pubmed: 28259757google scholar: lookup
  15. Pawlina-Tyszko K, Gurgul A, Szmatoła T, Ropka-Molik K, Semik-Gurgul E, Klukowska-Rötzler J, Koch C, Mählmann K, Bugno-Poniewierska M. Genomic landscape of copy number variation and copy neutral loss of heterozygosity events in equine sarcoids reveals increased instability of the sarcoid genome.. Biochimie 2017 Sep;140:122-132.
    doi: 10.1016/j.biochi.2017.07.006pubmed: 28743673google scholar: lookup
  16. Unger L, Jagannathan V, Pacholewska A, Leeb T, Gerber V. Differences in miRNA differential expression in whole blood between horses with sarcoid regression and progression.. J Vet Intern Med 2019 Jan;33(1):241-250.
    doi: 10.1111/jvim.15375pmc: PMC6335546pubmed: 30506726google scholar: lookup
  17. Unger L, Gerber V, Pacholewska A, Leeb T, Jagannathan V. MicroRNA fingerprints in serum and whole blood of sarcoid-affected horses as potential non-invasive diagnostic biomarkers.. Vet Comp Oncol 2019 Mar;17(1):107-117.
    doi: 10.1111/vco.12451pubmed: 30430738google scholar: lookup
  18. Taylor S, Haldorson G. A review of equine sarcoid. Equine Veter-Educ. 2012;25:210–216.
    doi: 10.1111/j.2042-3292.2012.00411.xgoogle scholar: lookup
  19. Hanahan D, Weinberg RA. The hallmarks of cancer.. Cell 2000 Jan 7;100(1):57-70.
    doi: 10.1016/S0092-8674(00)81683-9pubmed: 10647931google scholar: lookup
  20. Lambert AW, Pattabiraman DR, Weinberg RA. Emerging Biological Principles of Metastasis.. Cell 2017 Feb 9;168(4):670-691.
    doi: 10.1016/j.cell.2016.11.037pmc: PMC5308465pubmed: 28187288google scholar: lookup
  21. Tomasek JJ, Haaksma CJ, Eddy RJ, Vaughan MB. Fibroblast contraction occurs on release of tension in attached collagen lattices: dependency on an organized actin cytoskeleton and serum.. Anat Rec 1992 Mar;232(3):359-68.
    doi: 10.1002/ar.1092320305pubmed: 1543260google scholar: lookup
  22. Teifke JP, Kidney BA, Löhr CV, Yager JA. Detection of papillomavirus-DNA in mesenchymal tumour cells and not in the hyperplastic epithelium of feline sarcoids.. Vet Dermatol 2003 Feb;14(1):47-56.
    doi: 10.1046/j.1365-3164.2003.00324.xpubmed: 12603685google scholar: lookup
  23. Primer3. [(accessed on 17 July 2017)]; Available online: http://bioinfo.ut.ee/primer3-0.4.0.
  24. Pfaffl MW. A new mathematical model for relative quantification in real-time RT-PCR.. Nucleic Acids Res 2001 May 1;29(9):e45.
    doi: 10.1093/nar/29.9.e45pmc: PMC55695pubmed: 11328886google scholar: lookup
  25. . The R Project for Statistical Computing. [(accessed on 13 February 2012)]; Available online: http://www.r-project.org/
  26. Fife CM, McCarroll JA, Kavallaris M. Movers and shakers: cell cytoskeleton in cancer metastasis.. Br J Pharmacol 2014 Dec;171(24):5507-23.
    doi: 10.1111/bph.12704pmc: PMC4290699pubmed: 24665826google scholar: lookup
  27. Stengel K, Zheng Y. Cdc42 in oncogenic transformation, invasion, and tumorigenesis.. Cell Signal 2011 Sep;23(9):1415-23.
    doi: 10.1016/j.cellsig.2011.04.001pmc: PMC3115433pubmed: 21515363google scholar: lookup
  28. Guo Y, Kenney SR, Muller CY, Adams S, Rutledge T, Romero E, Murray-Krezan C, Prekeris R, Sklar LA, Hudson LG, Wandinger-Ness A. R-Ketorolac Targets Cdc42 and Rac1 and Alters Ovarian Cancer Cell Behaviors Critical for Invasion and Metastasis.. Mol Cancer Ther 2015 Oct;14(10):2215-27.
    doi: 10.1158/1535-7163.MCT-15-0419pmc: PMC4596774pubmed: 26206334google scholar: lookup
  29. Abreu-Velez AM, Howard MS. Collagen IV in Normal Skin and in Pathological Processes.. N Am J Med Sci 2012 Jan;4(1):1-8.
    doi: 10.4103/1947-2714.92892pmc: PMC3289483pubmed: 22393540google scholar: lookup
  30. Vihinen P, Kähäri VM. Matrix metalloproteinases in cancer: prognostic markers and therapeutic targets.. Int J Cancer 2002 May 10;99(2):157-66.
    doi: 10.1002/ijc.10329pubmed: 11979428google scholar: lookup
  31. Yuan Z, Gobeil PA, Campo MS, Nasir L. Equine sarcoid fibroblasts over-express matrix metalloproteinases and are invasive.. Virology 2010 Jan 5;396(1):143-51.
    doi: 10.1016/j.virol.2009.10.010pubmed: 19896685google scholar: lookup
  32. Anania MC, Sensi M, Radaelli E, Miranda C, Vizioli MG, Pagliardini S, Favini E, Cleris L, Supino R, Formelli F, Borrello MG, Pierotti MA, Greco A. TIMP3 regulates migration, invasion and in vivo tumorigenicity of thyroid tumor cells.. Oncogene 2011 Jul 7;30(27):3011-23.
    doi: 10.1038/onc.2011.18pubmed: 21339735google scholar: lookup
  33. Adissu HA, McKerlie C, Di Grappa M, Waterhouse P, Xu Q, Fang H, Khokha R, Wood GA. Timp3 loss accelerates tumour invasion and increases prostate inflammation in a mouse model of prostate cancer.. Prostate 2015 Dec;75(16):1831-43.
    doi: 10.1002/pros.23056pubmed: 26332574google scholar: lookup
  34. Williams HC, San Martín A, Adamo CM, Seidel-Rogol B, Pounkova L, Datla SR, Lassègue B, Bear JE, Griendling K. Role of coronin 1B in PDGF-induced migration of vascular smooth muscle cells.. Circ Res 2012 Jun 22;111(1):56-65.
    doi: 10.1161/CIRCRESAHA.111.255745pmc: PMC3412545pubmed: 22619279google scholar: lookup
  35. Kim GY, Park JH, Kim H, Lim HJ, Park HY. Coronin 1B serine 2 phosphorylation by p38α is critical for vascular endothelial growth factor-induced migration of human umbilical vein endothelial cells.. Cell Signal 2016 Dec;28(12):1817-1825.
    doi: 10.1016/j.cellsig.2016.08.009pubmed: 27592029google scholar: lookup
  36. Priya R, Wee K, Budnar S, Gomez GA, Yap AS, Michael M. Coronin 1B supports RhoA signaling at cell-cell junctions through Myosin II.. Cell Cycle 2016 Nov 16;15(22):3033-3041.
    doi: 10.1080/15384101.2016.1234549pmc: PMC5134703pubmed: 27650961google scholar: lookup
  37. Dave JM, Bayless KJ. Vimentin as an integral regulator of cell adhesion and endothelial sprouting.. Microcirculation 2014 May;21(4):333-44.
    doi: 10.1111/micc.12111pubmed: 24387004google scholar: lookup
  38. Battaglia RA, Delic S, Herrmann H, Snider NT. Vimentin on the move: new developments in cell migration.. F1000Res 2018;7.
    doi: 10.12688/f1000research.15967.1pmc: PMC6241562pubmed: 30505430google scholar: lookup

Citations

This article has been cited 1 times.
  1. Podstawski P, Ropka-Molik K, Semik-Gurgul E, Samiec M, Skrzyszowska M, Podstawski Z, Szmatoła T, Witkowski M, Pawlina-Tyszko K. Assessment of BPV-1 Mediated Matrix Metalloproteinase Genes Deregulation in the In Vivo and In Vitro Models Designed to Explore Molecular Nature of Equine Sarcoids. Cells 2022 Apr 8;11(8).
    doi: 10.3390/cells11081268pubmed: 35455948google scholar: lookup
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