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Home / Equine Research Bank / Allergy / Molecular allergen profiling in horses by microarray reveals...
Allergy2018; 73(7); 1436-1446; doi: 10.1111/all.13417

Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.

Abstract: Companion animals are also affected by IgE-mediated allergies, but the eliciting molecules are largely unknown. We aimed at refining an allergen microarray to explore sensitization in horses and compare it to the human IgE reactivity profiles. Methods: Custom-designed allergen microarray was produced on the basis of the ImmunoCAP ISAC technology containing 131 allergens. Sera from 51 horses derived from Europe or Japan were tested for specific IgE reactivity. The included horse patients were diagnosed for eczema due to insect bite hypersensitivity, chronic coughing, recurrent airway obstruction and urticaria or were clinically asymptomatic. Results: Horses showed individual IgE-binding patterns irrespective of their health status, indicating sensitization. In contrast to European and Japanese human sensitization patterns, frequently recognized allergens were Aln g 1 from alder and Cyn d 1 from Bermuda grass, likely due to specific respiratory exposure around paddocks and near the ground. The most prevalent allergen for 72.5% of the tested horses (37/51) was the 2S-albumin Fag e 2 from buckwheat, which recently gained importance not only in human but also in horse diet. Conclusions: In line with the One Health concept, covering human health, animal health and environmental health, allergen microarrays provide novel information on the allergen sensitization patterns of the companion animals around us, which may form a basis for allergen-specific preventive and therapeutic concepts.
© 2018 The Authors. Allergy Published by John Wiley & Sons Ltd.
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Publication Date: 2018-02-27 PubMed ID: 29350763PubMed Central: PMC6032949DOI: 10.1111/all.13417Google Scholar: Lookup
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  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on studying the allergens affecting horses by utilizing a specially constructed allergen microarray technology. The researchers found that the most common allergen (72.5% prevalence) was Fag e 2 (2S-albumin) from buckwheat, suggesting implications on horse diet.

Research Methodology

  • The research was aimed at identifying potential allergens in horses using a custom microarray based on ImmunoCAP ISAC technology, a type of biochip used to measure the presence of certain proteins in the serum associated with allergic reactions.
  • The array contained 131 diverse allergens, providing a comprehensive platform to study sensitization in horses.
  • Fifty-one horses from Europe and Japan, diagnosed with various conditions like insect bite hypersensitivity, chronic coughing, recurrent airway obstruction and urticaria, or those that were clinically asymptomatic, were involved in the study.

Results and Findings

  • The study revealed that irrespective of the health status of the horses, individual IgE-binding patterns indicative of sensitization were observed.
  • Unlike the human sensitization patterns in Europe and Japan, common allergens recognized were Aln g 1 from alder and Cyn d 1 from Bermuda grass. This is probably due to the specific exposure of horses to these substances in their environments, like around paddocks and near the ground.
  • The most significant finding was that Fag e 2 (2S-albumin) from buckwheat emerged as the most common sensitizing allergen in 72.5% of the tested horses.
  • The implication of this finding is significant since buckwheat has recently gained importance not only in human diet but also in horse diet.

Conclusion and Implications

  • The study underscores the utility of allergen microarrays in providing novel information about allergen sensitization patterns in animals. This is in line with the One Health concept that encompasses human health, animal health, and environmental health.
  • This information can act as a springboard for developing allergen-specific preventive strategies and therapeutic concepts.

Cite This Article

APA
Einhorn L, Hofstetter G, Brandt S, Hainisch EK, Fukuda I, Kusano K, Scheynius A, Mittermann I, Resch-Marat Y, Vrtala S, Valenta R, Marti E, Rhyner C, Crameri R, Satoh R, Teshima R, Tanaka A, Sato H, Matsuda H, Pali-Schöll I, Jensen-Jarolim E. (2018). Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer. Allergy, 73(7), 1436-1446. https://doi.org/10.1111/all.13417

Publication

Allergy
ISSN: 1398-9995
NlmUniqueID: 7804028
Country: Denmark
Language: English
Volume: 73
Issue: 7
Pages: 1436-1446

Researcher Affiliations

Einhorn, L
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Hofstetter, G
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
Brandt, S
  • Research Group Oncology, Equine Clinic, University of Veterinary Medicine Vienna, Vienna, Austria.
Hainisch, E K
  • Research Group Oncology, Equine Clinic, University of Veterinary Medicine Vienna, Vienna, Austria.
Fukuda, I
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Mikoma, Japan.
Kusano, K
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Mikoma, Japan.
Scheynius, A
  • Science for Life Laboratory, Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, Södersjukhuset, Stockholm, Sweden.
Mittermann, I
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Resch-Marat, Y
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Vrtala, S
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Valenta, R
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Marti, E
  • Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Rhyner, C
  • Swiss Institute for Allergy and Asthma Research (SIAF), Davos, Switzerland.
Crameri, R
  • Swiss Institute for Allergy and Asthma Research (SIAF), Davos, Switzerland.
Satoh, R
  • Division of Food Function Research, Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan.
Teshima, R
  • National Institute of Health Sciences, Tokyo, Japan.
Tanaka, A
  • Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Sato, H
  • Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Matsuda, H
  • Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Pali-Schöll, I
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Jensen-Jarolim, E
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
  • AllergyCare, Allergy Diagnosis and Study Center, Vienna, Austria.

MeSH Terms

  • Allergens / immunology
  • Animals
  • Antigens, Plant / immunology
  • Epitope Mapping / methods
  • Epitopes / genetics
  • Epitopes / immunology
  • Fagopyrum / adverse effects
  • Female
  • Horses
  • Humans
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Male

Grant Funding

  • P 26728 / Austrian Science Fund FWF

References

This article includes 84 references
  1. Gershwin LJ. Comparative immunology of allergic responses.. Annu Rev Anim Biosci 2015;3:327‐346.
    pubmed: 25422854
  2. Jensen‐Jarolim E, Einhorn L, Herrmann I, Thalhammer JG, Panakova L. Pollen allergies in humans and their dogs, cats and horses: differences and similarities.. Clin Transl Allergy 2015;5:15.
    pmc: PMC4387677pubmed: 25852853
  3. Pali‐Scholl I, De Lucia M, Jackson H, Janda J, Mueller RS, Jensen‐Jarolim E. Comparing immediate‐type food allergy in humans and companion animals‐revealing unmet needs.. Allergy 2017;72:1643‐1656.
    pubmed: 28394404
  4. Davis MF, Rankin SC, Schurer JM. Checklist for One Health Epidemiological Reporting of Evidence (COHERE).. One Health 2017;4:14‐21.
    pmc: PMC5536878pubmed: 28825424
  5. Fadok VA. Update on equine allergies.. Vet Clin North Am Equine Pract 2013;29:541‐550.
    pubmed: 24267673
  6. Jensen‐Jarolim E, Herrmann I, Panakova L, Janda J. Allergic and Atopic Eczema in Humans and Their Animals. In: Jensen‐Jarolim E, ed. Comparative Medicine: diseases linking humans with their animals. Switzerland: Springer International Publishing; 2017:131‐150.
  7. Volland‐Francqueville M, Sabbah A. Recurrent or chronic urticaria in thoroughbred race‐horses: clinical observations.. Eur Ann Allergy Clin Immunol 2004;36:9‐12.
    pubmed: 15015746
  8. Hufnagl K, Hirt R, Robibaro B. Out of Breath: asthma in Humans and Their Animals. In: Jensen‐Jarolim E, ed. Comparative Medicine: diseases linking Humans with their animals: Switzerland: Springer International; 2017:71‐86.
  9. Couetil LL, Cardwell JM, Gerber V, Lavoie JP, Leguillette R, Richard EA. Inflammatory airway disease of horses–revised consensus statement.. J Vet Intern Med 2016;30:503‐515.
    pmc: PMC4913592pubmed: 26806374
  10. McGorum BC, Dixon PM, Halliwell RE. Quantification of histamine in plasma and pulmonary fluids from horses with chronic obstructive pulmonary disease, before and after ‘natural (hay and straw) challenges’.. Vet Immunol Immunopathol 1993;36:223‐237.
    pubmed: 8506613
  11. Tahon L, Baselgia S, Gerber V. In vitro allergy tests compared to intradermal testing in horses with recurrent airway obstruction.. Vet Immunol Immunopathol 2009;127:85‐93.
    pubmed: 19027178
  12. Schaffartzik A, Hamza E, Janda J, Crameri R, Marti E, Rhyner C. Equine insect bite hypersensitivity: what do we know?. Vet Immunol Immunopathol 2012;147:113‐126.
    pubmed: 22575371
  13. Fadok VA, Greiner EC. Equine insect hypersensitivity: skin test and biopsy results correlated with clinical data.. Equine Vet J 1990;22:236‐240.
    pubmed: 1976506
  14. Lanz S, Brunner A, Graubner C, Marti E, Gerber V. Insect Bite Hypersensitivity in Horses is Associated with Airway Hyperreactivity.. J Vet Intern Med 2017;31:1877‐1883.
    pmc: PMC5697210pubmed: 28921663
  15. Han H, Roan F, Ziegler SF. The atopic march: current insights into skin barrier dysfunction and epithelial cell‐derived cytokines.. Immunol Rev 2017;278:116‐130.
    pmc: PMC5492959pubmed: 28658558
  16. Wallace JC, Vogelnest LJ. Evaluation of the presence of house dust mites in horse rugs.. Vet Dermatol 2010;21:602‐607.
    pubmed: 20529010
  17. Stepnik CT, Outerbridge CA, White SD, Kass PH. Equine atopic skin disease and response to allergen‐specific immunotherapy: a retrospective study at the University of California‐Davis (1991‐2008).. Vet Dermatol 2012;23:29‐35.
    pubmed: 21777308
  18. Pali‐Schöll I, Herrmann I, Jensen‐Jarolim E, Iben C. Allergies, with Focus on Food Allergies, in Humans and Their Animals. In: Jensen‐Jarolim E, editor. Comparative Medicine. Disorders linking humans with their animals: Springer International Publishing Switzerland; 2017: pp 109‐129.
  19. Marsella R. Equine allergy therapy: update on the treatment of environmental, insect bite hypersensitivity, and food allergies.. Vet Clin North Am Equine Pract 2013;29:551‐557.
    pubmed: 24267674
  20. Mueller R, Jensen‐Jarolim E, F R‐W. Comparative allergology: Allergen immunotherapy in dogs, cats and horses compared to current practice in humans. EAACI position paper.. Allergy 2017;72:1643‐1656.
    pubmed: 0
  21. Halldorsdottir S, Larsen HJ, Mehl R. Intradermal challenge of Icelandic horses with extracts of four species of the genus Culicoides.. Res Vet Sci 1989;47:283‐287.
    pubmed: 2595084
  22. Lorch G, Hillier A, Kwochka KW, Saville WJ, Kohn CW, LeRoy BE. Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with and without atopy.. J Am Vet Med Assoc 2001;218:1314‐1322.
    pubmed: 11330620
  23. Lebis C, Bourdeau P, Marzin‐Keller F. Intradermal skin tests in equine dermatology: a study of 83 horses.. Equine Vet J 2002;34:666‐671.
    pubmed: 12455836
  24. Wagner B, Miller WH, Morgan EE. IgE and IgG antibodies in skin allergy of the horse.. Vet Res 2006;37:813‐825.
    pubmed: 16973120
  25. Eder C, Crameri R, Mayer C. Allergen‐specific IgE levels against crude mould and storage mite extracts and recombinant mould allergens in sera from horses affected with chronic bronchitis.. Vet Immunol Immunopathol 2000;73:241‐253.
    pubmed: 10713338
  26. Frey R, Bergvall K, Egenvall A. Allergen‐specific IgE in Icelandic horses with insect bite hypersensitivity and healthy controls, assessed by FcepsilonR1alpha‐based serology.. Vet Immunol Immunopathol 2008;126:102‐109.
    pubmed: 18676025
  27. Ziegler A, Hamza E, Jonsdottir S. Longitudinal analysis of allergen‐specific IgE and IgG subclasses as potential predictors of insect bite hypersensitivity following first exposure to Culicoides in Icelandic horses.. Vet Dermatol 2018;29:51‐e22.
    pubmed: 28980353
  28. Canonica GW, Ansotegui IJ, Pawankar R. A WAO ‐ ARIA ‐ GA(2)LEN consensus document on molecular‐based allergy diagnostics.. World Allergy Organ J 2013;6:17.
    pmc: PMC3874689pubmed: 24090398
  29. Matricardi PM, Kleine‐Tebbe J, Hoffmann HJ. EAACI Molecular Allergology User's Guide.. Pediatr Allergy Immunol 2016;27(Suppl 23):1‐250.
    pubmed: 27288833
  30. Jensen‐Jarolim E, Jensen AN, Canonica GW. Debates in allergy medicine: molecular allergy diagnosis with ISAC will replace screenings by skin prick test in the future.. World Allergy Organ J 2017;10:33.
    pmc: PMC5604284pubmed: 28959378
  31. Larenas‐Linnemann D, Luna‐Pech JA, Mosges R. Debates in allergy medicine: allergy skin testing cannot be replaced by molecular diagnosis in the near future.. World Allergy Organ J 2017;10:32.
    pmc: PMC5604190pubmed: 29043011
  32. Harwanegg C, Laffer S, Hiller R. Microarrayed recombinant allergens for diagnosis of allergy.. Clin Exp Allergy 2003;33:7‐13.
    pubmed: 12534543
  33. Hiller R, Laffer S, Harwanegg C. Microarrayed allergen molecules: diagnostic gatekeepers for allergy treatment.. FASEB J 2002;16:414‐416.
    pubmed: 11790727
  34. Lupinek C, Wollmann E, Baar A. Advances in allergen‐microarray technology for diagnosis and monitoring of allergy: the MeDALL allergen‐chip.. Methods 2014;66:106‐119.
    pmc: PMC4687054pubmed: 24161540
  35. Mueller RS, Janda J, Jensen‐Jarolim E, Rhyner C, Marti E. Allergens in veterinary medicine.. Allergy 2016;71:27‐35.
    pmc: PMC4716287pubmed: 26280544
  36. Mittermann I, Reininger R, Zimmermann M. The IgE‐reactive autoantigen Hom s 2 induces damage of respiratory epithelial cells and keratinocytes via induction of IFN‐gamma.. J Invest Dermatol 2008;128:1451‐1459.
    pubmed: 18079747
  37. Gioti A, Nystedt B, Li W. Genomic insights into the atopic eczema‐associated skin commensal yeast Malassezia sympodialis.. MBio 2013;4:e00572‐00512.
    pmc: PMC3560662pubmed: 23341551
  38. Saunders CW, Scheynius A, Heitman J. Malassezia fungi are specialized to live on skin and associated with dandruff, eczema, and other skin diseases.. PLoS Pathog 2012;8:e1002701.
    pmc: PMC3380954pubmed: 22737067
  39. Schaffartzik A, Marti E, Crameri R, Rhyner C. Cloning, production and characterization of antigen 5 like proteins from Simulium vittatum and Culicoides nubeculosus, the first cross‐reactive allergen associated with equine insect bite hypersensitivity.. Vet Immunol Immunopathol 2010;137:76‐83.
    pubmed: 20537727
  40. Schaffartzik A, Marti E, Torsteinsdottir S, Mellor PS, Crameri R, Rhyner C. Selective cloning, characterization, and production of the Culicoides nubeculosus salivary gland allergen repertoire associated with equine insect bite hypersensitivity.. Vet Immunol Immunopathol 2011;139:200‐209.
    pubmed: 21071100
  41. O'Neil SE, Heinrich TK, Hales BJ. The chitinase allergens Der p 15 and Der p 18 from Dermatophagoides pteronyssinus.. Clin Exp Allergy 2006;36:831‐839.
    pubmed: 16776685
  42. Resch Y, Blatt K, Malkus U. Molecular, structural and immunological characterization of Der p 18, a chitinase‐like house dust mite allergen.. PLoS One 2016;11:e0160641.
    pmc: PMC4993390pubmed: 27548813
  43. Stremnitzer C, Manzano‐Szalai K, Willensdorfer A. Papain Degrades Tight Junction Proteins of Human Keratinocytes In Vitro and Sensitizes C57BL/6 Mice via the Skin Independent of its Enzymatic Activity or TLR4 Activation.. J Invest Dermatol 2015;135:1790‐1800.
    pmc: PMC4471117pubmed: 25705851
  44. Lukschal A, Wallmann J, Bublin M. Mimotopes for Api g 5, a Relevant Cross‐reactive Allergen, in the Celery‐Mugwort‐Birch‐Spice Syndrome.. Allergy Asthma Immunol Res 2016;8:124‐131.
    pmc: PMC4713875pubmed: 26739405
  45. McCall C, Hunter S, Stedman K. Characterization and cloning of a major high molecular weight house dust mite allergen (Der f 15) for dogs.. Vet Immunol Immunopathol 2001;78:231‐247.
    pubmed: 11292526
  46. Weber E, Hunter S, Stedman K. Identification, characterization, and cloning of a complementary DNA encoding a 60‐kd house dust mite allergen (Der f 18) for human beings and dogs.. J Allergy Clin Immunol 2003;112:79‐86.
    pubmed: 12847483
  47. van der Meide NM, Savelkoul HF, Meulenbroeks C, Ducro BJ, Tijhaar E. Evaluation of a diagnostic ELISA for insect bite hypersensitivity in horses using recombinant Obsoletus complex allergens.. Vet J 2014;200:31‐37.
    pubmed: 24703873
  48. Wagner B, Miller WH Jr, Erb HN, Lunn DP, Antczak DF. Sensitization of skin mast cells with IgE antibodies to Culicoides allergens occurs frequently in clinically healthy horses.. Vet Immunol Immunopathol 2009;132:53‐61.
    pubmed: 19836083
  49. Wagner B, Radbruch A, Rohwer J, Leibold W. Monoclonal anti‐equine IgE antibodies with specificity for different epitopes on the immunoglobulin heavy chain of native IgE.. Vet Immunol Immunopathol 2003;92:45‐60.
    pubmed: 12628763
  50. Marti E, Ehrensperger F, Burger D, Ousey J, Day MJ, Wilson AD. Maternal transfer of IgE and subsequent development of IgE responses in the horse (Equus callabus).. Vet Immunol Immunopathol 2009;127:203‐211.
    pubmed: 19081642
  51. Morgan EE, Miller WH Jr, Wagner B. A comparison of intradermal testing and detection of allergen‐specific immunoglobulin E in serum by enzyme‐linked immunosorbent assay in horses affected with skin hypersensitivity.. Vet Immunol Immunopathol 2007;120:160‐167.
    pubmed: 17888519
  52. Gadisseur R, Chapelle J‐P, Cavalier E. A new tool in the field of in‐vitro diagnosis of allergy: preliminary results in the comparison of ImmunoCAP© 250 with the ImmunoCAP© ISAC.. Clin Chem Lab Med 2011;49:277‐280.
    pubmed: 21143018
  53. Finkina EI, Melnikova DN, Bogdanov IV, Ovchinnikova TV. Plant pathogenesis‐related proteins PR‐10 and PR‐14 as components of innate immunity system and ubiquitous allergens.. Curr Med Chem 2017;24:1772‐1787.
    pubmed: 27784212
  54. Roth‐Walter F, Gomez‐Casado C, Pacios LF. Bet v 1 from birch pollen is a lipocalin‐like protein acting as allergen only when devoid of iron by promoting Th2 lymphocytes.. J Biol Chem 2014;289:17416‐17421.
    pmc: PMC4067174pubmed: 24798325
  55. Hauser M, Asam C, Himly M. Bet v 1‐like pollen allergens of multiple Fagales species can sensitize atopic individuals.. Clin Exp Allergy 2011;41:1804‐1814.
    pmc: PMC5689382pubmed: 22092996
  56. Scala E, Abeni D, Cecchi L. Molecular recognition profiles and clinical patterns of PR‐10 Sensitization in a Birch‐Free Mediterranean Area.. Int Arch Allergy Immunol 2017;173:138‐146.
    pubmed: 28787736
  57. Homann A, Schramm G, Jappe U. Glycans and glycan‐specific IgE in clinical and molecular allergology: sensitization, diagnostics, and clinical symptoms.. J Allergy Clin Immunol 2017;140:356‐368.
    pubmed: 28479330
  58. Mattsson L, Lundgren T, Everberg H, Larsson H, Lidholm J. Prostatic kallikrein: a new major dog allergen.. J Allergy Clin Immunol 2009;123:362‐368.
    pubmed: 19135239
  59. Yi L, Cheng D, Zhang K. Intelectin contributes to allergen‐induced IL‐25, IL‐33, and TSLP expression and type 2 response in asthma and atopic dermatitis.. Mucosal Immunol 2017;10:1491‐1503.
    pmc: PMC5568519pubmed: 28224996
  60. Giuffrida MG, Villalta D, Mistrello G, Amato S, Asero R. Shrimp allergy beyond Tropomyosin in Italy: clinical relevance of Arginine Kinase, Sarcoplasmic calcium binding protein and Hemocyanin.. Eur Ann Allergy Clin Immunol 2014;46:172‐177.
    pubmed: 25224947
  61. Binder M, Mahler V, Hayek B. Molecular and immunological characterization of arginine kinase from the Indianmeal moth, Plodia interpunctella, a novel cross‐reactive invertebrate pan‐allergen.. J Immunol 2001;167:5470‐5477.
    pubmed: 11673567
  62. Gamez C, Zafra M, Boquete M. New shrimp IgE‐binding proteins involved in mite‐seafood cross‐reactivity.. Mol Nutr Food Res 2014;58:1915‐1925.
    pubmed: 24978201
  63. Jensen‐Jarolim E, Poulsen LK, With H, Kieffer M, Ottevanger V, Stahl SP. Atopic dermatitis of the face, scalp, and neck: type I reaction to the yeast Pityrosporum ovale?. J Allergy Clin Immunol 1992;89(1 Pt 1):44‐51.
    pubmed: 1730839
  64. Schmid‐Grendelmeier P, Scheynius A, Crameri R. The role of sensitization to Malassezia sympodialis in atopic eczema.. Chem Immunol Allergy 2006;91:98‐109.
    pubmed: 16354952
  65. Kekki OM, Scheynius A, Poikonen S, Koskinen A, Kautiainen H, Turjanmaa K. Sensitization to Malassezia in children with atopic dermatitis combined with food allergy.. Pediatr Allergy Immunol 2013;24:244‐249.
    pubmed: 23551180
  66. Shokri H. Occurrence and distribution of Malassezia species on skin and external ear canal of horses.. Mycoses 2016;59:28‐33.
    pubmed: 26549307
  67. Benkovic ET, Kreft S. Fagopyrins and protofagopyrins: detection, analysis, and potential phototoxicity in buckwheat.. J Agric Food Chem 2015;63:5715‐5724.
    pubmed: 26024291
  68. Sytar O, Brestic M, Zivcak M, Tran LS. The contribution of buckwheat genetic resources to health and dietary diversity.. Curr Genomics 2016;17:193‐206.
    pmc: PMC4869006pubmed: 27252586
  69. Bonafaccia G, Marocchini M, Kreft I. Composition and technological properties of the flour and bran from common and tartary buckwheat.. Food Chem 2003;80:9‐15.
  70. Christa K, Soral‐ŚMietana M. Buckwheat grains and buckwheat products ‐ nutritional and prophylactic value of their components ‐ a review.. Czech J Food Sci 2008;3:153‐162.
  71. Kayashita J, Shimaoka I, Nakajoh M, Yamazaki M, Kato N. Consumption of buckwheat protein lowers plasma cholesterol and raises fecal neutral sterols in cholesterol‐Fed rats because of its low digestibility.. J Nutr 1997;127:1395‐1400.
    pubmed: 9202097
  72. Kawa JM, Taylor CG, Przybylski R. Buckwheat concentrate reduces serum glucose in streptozotocin‐diabetic rats.. J Agric Food Chem 2003;51:7287‐7291.
    pubmed: 14640572
  73. Ma M‐S, Bae IY, Lee HG, Yang C‐B. Purification and identification of angiotensin I‐converting enzyme inhibitory peptide from buckwheat (Fagopyrum esculentum Moench).. Food Chem 2006;96:36‐42.
  74. Chan PK. Inhibition of tumor growth in vitro by the extract of fagopyrum cymosum (fago‐c).. Life Sci 2003;72:1851‐1858.
    pubmed: 12586222
  75. Choi SY, Sohn JH, Lee YW, Lee EK, Hong CS, Park JW. Application of the 16‐kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity.. Ann Allergy Asthma Immunol 2007;99:254‐260.
    pubmed: 17910329
  76. Tanaka K, Matsumoto K, Akasawa A. Pepsin‐resistant 16‐kD buckwheat protein is associated with immediate hypersensitivity reaction in patients with buckwheat allergy.. Int Arch Allergy Immunol 2002;129:49‐56.
    pubmed: 12372998
  77. Heffler E, Guida G, Badiu I, Nebiolo F, Rolla G. Anaphylaxis after eating Italian pizza containing buckwheat as the hidden food allergen.. J Investig Allergol Clin Immunol 2007;17:261‐263.
    pubmed: 17694699
  78. Heffler E, Nebiolo F, Asero R. Clinical manifestations, co‐sensitizations, and immunoblotting profiles of buckwheat‐allergic patients.. Allergy 2011;66:264‐270.
    pubmed: 20804471
  79. Wieslander G, Norback D. Buckwheat allergy.. Allergy 2001;56:703‐704.
    pubmed: 11488663
  80. Imamura T, Kanagawa Y, Ebisawa M. A survey of patients with self‐reported severe food allergies in Japan.. Pediatr Allergy Immunol 2008;19:270‐274.
    pubmed: 18397411
  81. Yang MS, Lee SH, Kim TW. Epidemiologic and clinical features of anaphylaxis in Korea.. Ann Allergy Asthma Immunol 2008;100:31‐36.
    pubmed: 18254479
  82. Asero R, Antonicelli L, Arena A. EpidemAAITO: features of food allergy in Italian adults attending allergy clinics: a multi‐centre study.. Clin Exp Allergy 2009;39:547‐555.
    pubmed: 19220321
  83. Badiu I, Olivieri E, Montagni M. Italian study on buckwheat allergy: prevalence and clinical features of buckwheat‐sensitized patients in Italy.. Int J Immunopathol Pharmacol 2013;26:801‐806.
    pubmed: 24067481
  84. Sammut D, Dennison P, Venter C, Kurukulaaratchy RJ. Buckwheat allergy: a potential problem in 21st century Britain.. BMJ Case Rep .
    doi: 10.1136/bcr.09.2011.4882pmc: PMC3214221pubmed: 22674117google scholar: lookup

Citations

This article has been cited 6 times.
  1. Ardicli S, Ardicli O, Yazici D, Pat Y, Babayev H, Xiong P, Zeyneloglu C, Garcia-Sanchez A, Shi LL, Viscardi OG, Skolnick S, Ogulur I, Dhir R, Jutel M, Agache I, Janda J, Pali-Schöll I, Nadeau KC, Akdis M, Akdis CA. Epithelial barrier dysfunction and associated diseases in companion animals: Differences and similarities between humans and animals and research needs. Allergy 2024 Dec;79(12):3238-3268.
    doi: 10.1111/all.16343pubmed: 39417247google scholar: lookup
  2. Norbäck D, Wieslander G. A Review on Epidemiological and Clinical Studies on Buckwheat Allergy. Plants (Basel) 2021 Mar 23;10(3).
    doi: 10.3390/plants10030607pubmed: 33806876google scholar: lookup
  3. Dezfouli SG, Mothes-Luksch N, Jensen AN, Untersmayr E, Kundi M, Jensen-Jarolim E. Linking cross-reactivity clusters of food and respiratory allergens in PAMD@ to asthma and duration of allergy. World Allergy Organ J 2020 Dec;13(12):100483.
    doi: 10.1016/j.waojou.2020.100483pubmed: 33294115google scholar: lookup
  4. Satoh R, Jensen-Jarolim E, Teshima R. Understanding buckwheat allergies for the management of allergic reactions in humans and animals. Breed Sci 2020 Mar;70(1):85-92.
    doi: 10.1270/jsbbs.19051pubmed: 32351307google scholar: lookup
  5. . A WAO - ARIA - GA(2)LEN consensus document on molecular-based allergy diagnosis (PAMD@): Update 2020. World Allergy Organ J 2020 Feb;13(2):100091.
    doi: 10.1016/j.waojou.2019.100091pubmed: 32180890google scholar: lookup
  6. White SJ, Moore-Colyer M, Marti E, Hannant D, Gerber V, Coüetil L, Richard EA, Alcocer M. Antigen array for serological diagnosis and novel allergen identification in severe equine asthma. Sci Rep 2019 Oct 23;9(1):15170.
    doi: 10.1038/s41598-019-51820-7pubmed: 31645629google scholar: lookup
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