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Allergy2018; 73(7); 1436-1446; doi: 10.1111/all.13417

Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer.

Abstract: Companion animals are also affected by IgE-mediated allergies, but the eliciting molecules are largely unknown. We aimed at refining an allergen microarray to explore sensitization in horses and compare it to the human IgE reactivity profiles. Methods: Custom-designed allergen microarray was produced on the basis of the ImmunoCAP ISAC technology containing 131 allergens. Sera from 51 horses derived from Europe or Japan were tested for specific IgE reactivity. The included horse patients were diagnosed for eczema due to insect bite hypersensitivity, chronic coughing, recurrent airway obstruction and urticaria or were clinically asymptomatic. Results: Horses showed individual IgE-binding patterns irrespective of their health status, indicating sensitization. In contrast to European and Japanese human sensitization patterns, frequently recognized allergens were Aln g 1 from alder and Cyn d 1 from Bermuda grass, likely due to specific respiratory exposure around paddocks and near the ground. The most prevalent allergen for 72.5% of the tested horses (37/51) was the 2S-albumin Fag e 2 from buckwheat, which recently gained importance not only in human but also in horse diet. Conclusions: In line with the One Health concept, covering human health, animal health and environmental health, allergen microarrays provide novel information on the allergen sensitization patterns of the companion animals around us, which may form a basis for allergen-specific preventive and therapeutic concepts.
© 2018 The Authors. Allergy Published by John Wiley & Sons Ltd.
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Publication Date: 2018-02-27 PubMed ID: 29350763PubMed Central: PMC6032949DOI: 10.1111/all.13417Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper focuses on studying the allergens affecting horses by utilizing a specially constructed allergen microarray technology. The researchers found that the most common allergen (72.5% prevalence) was Fag e 2 (2S-albumin) from buckwheat, suggesting implications on horse diet.

Research Methodology

  • The research was aimed at identifying potential allergens in horses using a custom microarray based on ImmunoCAP ISAC technology, a type of biochip used to measure the presence of certain proteins in the serum associated with allergic reactions.
  • The array contained 131 diverse allergens, providing a comprehensive platform to study sensitization in horses.
  • Fifty-one horses from Europe and Japan, diagnosed with various conditions like insect bite hypersensitivity, chronic coughing, recurrent airway obstruction and urticaria, or those that were clinically asymptomatic, were involved in the study.

Results and Findings

  • The study revealed that irrespective of the health status of the horses, individual IgE-binding patterns indicative of sensitization were observed.
  • Unlike the human sensitization patterns in Europe and Japan, common allergens recognized were Aln g 1 from alder and Cyn d 1 from Bermuda grass. This is probably due to the specific exposure of horses to these substances in their environments, like around paddocks and near the ground.
  • The most significant finding was that Fag e 2 (2S-albumin) from buckwheat emerged as the most common sensitizing allergen in 72.5% of the tested horses.
  • The implication of this finding is significant since buckwheat has recently gained importance not only in human diet but also in horse diet.

Conclusion and Implications

  • The study underscores the utility of allergen microarrays in providing novel information about allergen sensitization patterns in animals. This is in line with the One Health concept that encompasses human health, animal health, and environmental health.
  • This information can act as a springboard for developing allergen-specific preventive strategies and therapeutic concepts.

Cite This Article

APA
Einhorn L, Hofstetter G, Brandt S, Hainisch EK, Fukuda I, Kusano K, Scheynius A, Mittermann I, Resch-Marat Y, Vrtala S, Valenta R, Marti E, Rhyner C, Crameri R, Satoh R, Teshima R, Tanaka A, Sato H, Matsuda H, Pali-Schöll I, Jensen-Jarolim E. (2018). Molecular allergen profiling in horses by microarray reveals Fag e 2 from buckwheat as a frequent sensitizer. Allergy, 73(7), 1436-1446. https://doi.org/10.1111/all.13417

Publication

Allergy
ISSN: 1398-9995
NlmUniqueID: 7804028
Country: Denmark
Language: English
Volume: 73
Issue: 7
Pages: 1436-1446

Researcher Affiliations

Einhorn, L
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Hofstetter, G
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
Brandt, S
  • Research Group Oncology, Equine Clinic, University of Veterinary Medicine Vienna, Vienna, Austria.
Hainisch, E K
  • Research Group Oncology, Equine Clinic, University of Veterinary Medicine Vienna, Vienna, Austria.
Fukuda, I
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Mikoma, Japan.
Kusano, K
  • Racehorse Hospital, Miho Training Center, Japan Racing Association, Mikoma, Japan.
Scheynius, A
  • Science for Life Laboratory, Department of Clinical Science and Education, Karolinska Institutet, and Sachs' Children and Youth Hospital, Södersjukhuset, Stockholm, Sweden.
Mittermann, I
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Resch-Marat, Y
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Vrtala, S
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Valenta, R
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Marti, E
  • Department of Clinical Research and Veterinary Public Health, Vetsuisse Faculty, University of Bern, Bern, Switzerland.
Rhyner, C
  • Swiss Institute for Allergy and Asthma Research (SIAF), Davos, Switzerland.
Crameri, R
  • Swiss Institute for Allergy and Asthma Research (SIAF), Davos, Switzerland.
Satoh, R
  • Division of Food Function Research, Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan.
Teshima, R
  • National Institute of Health Sciences, Tokyo, Japan.
Tanaka, A
  • Laboratory of Comparative Animal Medicine, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Sato, H
  • Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Matsuda, H
  • Laboratory of Veterinary Molecular Pathology and Therapeutics, Division of Animal Life Science, Tokyo University of Agriculture and Technology, Fuchu, Japan.
Pali-Schöll, I
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
Jensen-Jarolim, E
  • The interuniversity Messerli Research Institute, University of Veterinary Medicine Vienna, Medical University Vienna and University Vienna, Vienna, Austria.
  • Institute of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria.
  • AllergyCare, Allergy Diagnosis and Study Center, Vienna, Austria.

MeSH Terms

  • Allergens / immunology
  • Animals
  • Antigens, Plant / immunology
  • Epitope Mapping / methods
  • Epitopes / genetics
  • Epitopes / immunology
  • Fagopyrum / adverse effects
  • Female
  • Horses
  • Humans
  • Immunoglobulin E / blood
  • Immunoglobulin E / immunology
  • Male

Grant Funding

  • P 26728 / Austrian Science Fund FWF

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