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Home / Equine Research Bank / Int J Antimicrob Agents / Molecular basis of resistance to macrolides and lincosamides...
International journal of antimicrobial agents2007; 29(5); 528-535; doi: 10.1016/j.ijantimicag.2006.12.016

Molecular basis of resistance to macrolides and lincosamides among staphylococci and streptococci from various animal sources collected in the resistance monitoring program BfT-GermVet.

Abstract: In this study, erythromycin- and/or clindamycin-resistant isolates among 248 coagulase-positive and coagulase-variable staphylococci and 500 streptococci, collected all over Germany during 2004-2006 in the resistance monitoring program BfT-GermVet, were investigated for their genetic basis of macrolide and/or lincosamide resistance. Staphylococci were sampled from various disease conditions of dogs/cats or pigs, whereas streptococci were from dogs/cats, pigs or horses. Resistant staphylococci were further identified biochemically to species and subspecies level and tested for the resistance genes erm(A), erm(B), erm(C), erm(TR), msr(A), msr(D), mef(A), mph(C), lnu(A), lnu(B) and lnu(C). The methylase genes erm(A), erm(B) and erm(C) were detected in staphylococci, alone or in different combinations. The erm(B) gene was the predominant gene in Staphylococcus intermedius and streptococci. The efflux gene msr(A) and the genes mph(C) and lnu(A) coding for inactivating enzymes were detected in single staphylococcal isolates. The efflux genes mef(A) and msr(D) were detected in three streptococci, in one of them together with the erm(B) gene. The lnu(B) gene was detected in seven porcine streptococcal isolates with reduced susceptibility to clindamycin. These data confirm that high-level resistance to erythromycin and clindamycin in staphylococci and streptococci was mainly due to rRNA methylases. The lnu(B) gene was detected for the first time in streptococci of animal origin.
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Publication Date: 2007-02-20 PubMed ID: 17307339DOI: 10.1016/j.ijantimicag.2006.12.016Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines the genetic factors that contribute to staphylococci and streptococci bacteria’s resistance to certain antibiotics in various animals. The researchers found that the resistance to antibiotics like erythromycin and clindamycin is mainly due to specific elements, known as rRNA methylases, within their genetic makeup.

Introduction and Methodology

  • The research focused on 248 coagulase-positive and coagulase-variable staphylococci and 500 streptococci, which were collected from Germany between 2004 and 2006 through the resistance monitoring program BfT-GermVet.
  • The staphylococci were obtained from dogs/cats or pigs with various disease conditions, while the streptococci were taken from dogs/cats, pigs, or horses.
  • The resistant staphylococci were then identified biologically to species and subspecies level. After this, the staphylococci were examined for the presence of specific resistance genes.

Findings and Conclusion

  • The research found certain resistance genes, namely erm(A), erm(B), and erm(C), in the staphylococci samples. These genes were either present individually or in different combinations.
  • The erm(B) gene was most commonly found in a particular type of staphylococci known as Staphylococcus intermedius, as well as among the streptococci sampled.
  • Similarly, the efflux gene msr(A) and the genes mph(C) and lnu(A), which code for inactivating enzymes were found in some staphylococci. Additionally, the efflux genes mef(A) and msr(D) were detected in three streptococci.
  • The lnu(B) gene, which seems to reduce susceptibility to the antibiotic clindamycin, was found in seven porcine streptococcal isolates. This was the first time this gene was detected in animal-derived streptococci.
  • From their data, the researchers concluded that high-level resistance to the antibiotics erythromycin and clindamycin in staphylococci and streptococci is primarily due to rRNA methylases, a key component of these resistance genes.

Cite This Article

APA
Lüthje P, Schwarz S. (2007). Molecular basis of resistance to macrolides and lincosamides among staphylococci and streptococci from various animal sources collected in the resistance monitoring program BfT-GermVet. Int J Antimicrob Agents, 29(5), 528-535. https://doi.org/10.1016/j.ijantimicag.2006.12.016

Publication

International journal of antimicrobial agents
ISSN: 0924-8579
NlmUniqueID: 9111860
Country: Netherlands
Language: English
Volume: 29
Issue: 5
Pages: 528-535

Researcher Affiliations

Lüthje, Petra
  • Institut für Tierzucht, Bundesforschungsanstalt für Landwirtschaft (FAL), Höltystr. 10, 31535 Neustadt-Mariensee, Germany.
Schwarz, Stefan

    MeSH Terms

    • Animals
    • Anti-Bacterial Agents / pharmacology
    • Bacterial Infections / microbiology
    • Bacterial Infections / veterinary
    • DNA, Bacterial / genetics
    • Drug Resistance, Bacterial / genetics
    • Genes, Bacterial / genetics
    • Germany
    • Lincosamides
    • Macrolides / pharmacology
    • Microbial Sensitivity Tests
    • Population Surveillance
    • Reverse Transcriptase Polymerase Chain Reaction
    • Staphylococcus / drug effects
    • Staphylococcus / genetics
    • Streptococcus / drug effects
    • Streptococcus / genetics

    Citations

    This article has been cited 24 times.
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