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BMC veterinary research2021; 17(1); 131; doi: 10.1186/s12917-021-02832-x

Molecular characterization and antimicrobial resistance of potentially human-pathogenic Escherichia coli strains isolated from riding horses.

Abstract: Transmission of antimicrobial resistant and virulent Escherichia coli (E. coli) from animal to human has been considered as a public health concern. This study aimed to determine the phylogenetic background and prevalence of diarrheagenic E. coli and antimicrobial resistance in healthy riding-horses in Iran. In this research, the genes related to six main pathotypes of E. coli were screened. Also, genotypic and phenotypic antimicrobial resistance against commonly used antibiotics were studied, then phylo-grouping was performed on all the isolates. Results: Out of 65 analyzed isolates, 29.23 % (n = 19) were determined as STEC and 6.15 % (n = 4) as potential EPEC. The most prevalent antimicrobial resistance phenotypes were against amoxicillin/clavulanic acid (46.2 %) and ceftriaxone (38.5 %). bla was the most detected resistance gene (98.4 %) among the isolates and 26.15 % of the E. coli isolates were determined as multi-drug resistant (MDR). Three phylo-types including B1 (76.92 %), A (13.85 %) and D (3.08 %) were detected among the isolates. Conclusions: Due to the close interaction of horses and humans, these findings would place emphasis on the pathogenic and zoonotic potential of the equine strains and may help to design antimicrobial resistance stewardship programs to control the dissemination of virulent and multi-drug resistant E. coli strains in the community.
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Publication Date: 2021-03-25 PubMed ID: 33766016PubMed Central: PMC7992949DOI: 10.1186/s12917-021-02832-xGoogle Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper explores the antimicrobial resistance and virulence of Escherichia coli (E. coli) strains originating from riding horses in Iran. The study probes into the possibility of these horses acting as a source of dangerous bacteria, highlighting antibiotic resistance, making it a significant issue for public health.

Research Objectives

  • This study was conducted with the aim of determining the prevalence and phylogenetic background of diarrheagenic E. coli in healthy riding horses in Iran.
  • The research team analyzed the genes associated with six main pathotypes or pathogenic types of E. coli.
  • Furthermore, the researchers investigated both genotypic (based on genetic makeup) and phenotypic (observable characteristics) antimicrobial resistance among these bacteria against commonly used antibiotics.
  • Finally, they tried to categorize or ‘phylo-group’ all the bacterial isolates they studied.

Results

  • The research identified that roughly 30% of the analyzed isolates were Shiga toxin-producing E. coli (STEC) and about 6% were potentially Enteropathogenic E. coli (EPEC).
  • The most common antibiotic resistance was found against amoxicillin/clavulanic acid and ceftriaxone, affecting 46.2% and 38.5% of the isolates respectively.
  • The prevalent resistance gene found was bla, detected in 98.4% of the isolates.
  • About 26.15% E. coli isolates were identified as multi-drug resistant (MDR).
  • The identified isolates were mainly grouped into three phylo-types: B1 (77%), A (14%), and D (3%).

Conclusions

  • Based on the close interaction between horses and humans, the research places emphasis on the zoonotic (disease transferable from animals to humans) potential of equine E. coli strains.
  • The presence of highly virulent and multi-drug resistant strains adds to the public health concern, underscoring the threat of antibiotic resistance.
  • The findings of this study should inform the design of antimicrobial resistance stewardship programs, helping to control the spread of such strains in the community.

Cite This Article

APA
Reshadi P, Heydari F, Ghanbarpour R, Bagheri M, Jajarmi M, Amiri M, Alizade H, Badouei MA, Sahraei S, Adib N. (2021). Molecular characterization and antimicrobial resistance of potentially human-pathogenic Escherichia coli strains isolated from riding horses. BMC Vet Res, 17(1), 131. https://doi.org/10.1186/s12917-021-02832-x

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 17
Issue: 1
Pages: 131
PII: 131

Researcher Affiliations

Reshadi, Pouya
  • Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Heydari, Fatemeh
  • Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Ghanbarpour, Reza
  • Molecular Microbiology Research Group, Shahid Bahonar University of Kerman, Kerman, Iran.
Bagheri, Mahboube
  • Department of Food Science and Technology, Bardsir Faculty of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran.
Jajarmi, Maziar
  • Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran. maziar.jajarmi@uk.ac.ir.
Amiri, Mohadese
  • Mashhad Branch, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Mashhad, Iran.
Alizade, Hesam
  • Infectious and Tropical Diseases Research Center, Hormozgan Health Institute, Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
Badouei, Mahdi Askari
  • Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran.
Sahraei, Shademan
  • Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.
Adib, Nasrin
  • Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran.

MeSH Terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Drug Resistance, Bacterial
  • Drug Resistance, Multiple, Bacterial
  • Enteropathogenic Escherichia coli / isolation & purification
  • Escherichia coli / classification
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Horses / microbiology
  • Iran / epidemiology
  • Microbial Sensitivity Tests / veterinary
  • Shiga-Toxigenic Escherichia coli / isolation & purification

Conflict of Interest Statement

All authors declare that the authors have no competing interests as defined by BMC, or other interests that might be perceived to influence the results and/or discussion reported in this paper.

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Citations

This article has been cited 3 times.
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