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Parasites & vectors2016; 9(1); 561; doi: 10.1186/s13071-016-1844-3

Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China.

Abstract: Enterocytozoon bieneusi is one of the most prevalent causative species of diarrhea and enteric diseases in various hosts. E. bieneusi has been identified in humans, mammals, birds, rodents and reptiles in China, but few studies have reported E. bieneusi in horses. Therefore, the present study was conducted to assess the prevalence, molecular characteristics and zoonotic potential of E. bieneusi among horses in southwestern China. Three hundred and thirty-three fecal specimens were collected from horses on five farms in the Sichuan and Yunnan provinces of southwestern China. The prevalence of E. bieneusi was 22.5 % (75/333), as determined by nested polymerase chain reaction and sequencing analysis of the internal transcribed spacer region of the ribosomal RNA gene of E. bieneusi. Altogether, 10 genotypes were identified among the 75 E. bieneusi-positive samples: four of these genotypes were known (horse1, horse2, SC02 and D) and six were novel (SCH1-4 and YNH1-2). Multilocus sequence typing using three microsatellites (MS1, MS3 and MS7) and one minisatellite (MS4) revealed three, two, three and three genotypes at these four loci, respectively. In phylogenetic analysis, all the genotypes of E. bieneusi obtained in this study were clustered into three distinct groups: D, SC02 and SCH1-3 were clustered into group 1 (zoonotic potential); SCH4 was clustered into group 2 (cattle-hosted); whereas horse2, YNH1 and YNH2 were clustered into group 6 (unclear zoonotic potential). This is the first report of E. bieneusi among horses in southwestern China. This is also the first multilocus genotyping analysis using microsatellite and minisatellite markers of E. bieneusi in horses. The presence of genotype D, which was previously identified in humans, and genotypes SC02 and SCH1-3, which belong to potential zoonotic group 1, these results indicate that horses are a potential source of human E. bieneusi infections in China.
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Publication Date: 2016-10-25 PubMed ID: 27776551PubMed Central: PMC5078948DOI: 10.1186/s13071-016-1844-3Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article centers primarily around a study that investigates the prevalence, molecular traits, and possible zoonotic potential of Enterocytozoon bieneusi in horses within southwestern China, providing critical insights on the possible role of horses as sources of human E. bieneusi infections within the region.

Research Objectives and Methodology

  • The primary objective of this study was to assess the occurrence and molecular properties of Enterocytozoon bieneusi in horses in the southwestern parts of China, a geographical region where prior studies on this subject are limited.
  • As part of the research, 333 fecal samples were obtained from horses in five different farms across the Sichuan and Yunnan provinces.
  • The internal transcribed spacer (ITS) region of the E. bieneusi ribosomal RNA gene was analyzed through nested polymerase chain reaction and sequencing to determine its prevalence in the collected samples.
  • The study further conducted multilocus sequence typing by employing three microsatellites (MS1, MS3, and MS7) and a minisatellite (MS4) to identify the distinct genotypes.

Research Findings and Implications

  • This study found the prevalence of E. bieneusi to be 22.5% among the tested samples. From 75 E. bieneusi-positive samples, 10 distinct genotypes were discovered, namely horse1, horse2, SC02, D, SCH1-4, and YNH1-2.
  • Among these identified genotypes, four were already documented (horse1, horse2, SC02, and D), while the rest (SCH1-4 and YNH1-2) were newly discovered.
  • In a phylogenetic examination, it was determined that these genotypes fell into three distinct groups. Group 1 consisted of genotypes D, SC02, and SCH1-3, identified to possess potential zoonotic capability. The genotype SCH4 fell into Group 2, widely associated with cattle, while genotypes horse2, YNH1, and YNH2 formed group 6, which has unclear zoonotic potential.
  • This research constitutes the initial report of E. bieneusi among horses in southwestern China. It also represents the pioneering multilocus genotyping study of E. bieneusi employing microsatellite and minisatellite markers.
  • Importantly, the presence of genotype D, which was formerly recognized in humans, along with genotypes SC02 and SCH1-3 (falling under potential zoonotic group 1), indicates that horses may be possible sources of human E. bieneusi infections within China.

Cite This Article

APA
Deng L, Li W, Zhong Z, Gong C, Liu X, Huang X, Xiao L, Zhao R, Wang W, Feng F, Zhang Y, Hu Y, Fu H, He M, Zhang Y, Wu K, Peng G. (2016). Molecular characterization and multilocus genotypes of Enterocytozoon bieneusi among horses in southwestern China. Parasit Vectors, 9(1), 561. https://doi.org/10.1186/s13071-016-1844-3

Publication

Parasites & vectors
ISSN: 1756-3305
NlmUniqueID: 101462774
Country: England
Language: English
Volume: 9
Issue: 1
Pages: 561
PII: 561

Researcher Affiliations

Deng, Lei
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Li, Wei
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhong, Zhijun
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Gong, Chao
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Liu, Xuehan
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Huang, Xiangming
  • Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China.
Xiao, Li
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhao, Ruoxuan
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Wang, Wuyou
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Feng, Fan
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhang, Yue
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Hu, Yanchun
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Fu, Hualin
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
He, Min
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China.
Zhang, Yue
  • Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China.
Wu, Kongju
  • Chengdu Giant Panda Breeding Research Base, Chengdu, Sichuan Province, 625001, China. 646401864@qq.com.
Peng, Guangneng
  • The Key Laboratory of Animal Disease and Human Health of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan Province, 611130, China. pgn.sicau@163.com.

MeSH Terms

  • Animals
  • China / epidemiology
  • Cluster Analysis
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / chemistry
  • DNA, Ribosomal Spacer / genetics
  • Enterocytozoon / classification
  • Enterocytozoon / genetics
  • Enterocytozoon / isolation & purification
  • Feces / microbiology
  • Genotype
  • Horse Diseases / epidemiology
  • Horse Diseases / microbiology
  • Horses / microbiology
  • Microsporidiosis / epidemiology
  • Microsporidiosis / microbiology
  • Microsporidiosis / veterinary
  • Multilocus Sequence Typing
  • Phylogeny
  • Polymerase Chain Reaction
  • Prevalence
  • Sequence Analysis, DNA

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This article has been cited 25 times.
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