Molecular Characterization of Equine APRIL and its Expression Analysis During the Adipogenic Differentiation of Equine Adipose-Derived Stem Cell In Vitro.
Abstract: A proliferation inducing ligand (APRIL) is a member of the TNF superfamily. It shares two receptors with B-cell activating factor (BAFF), B-cell maturation antigen (BCMA), and transmembrane activator and CAML interactor (TACI). Herein, the equine APRIL was identified from equine adipose-derived stem cell (ASC), and the protein expression of APRIL and its related molecules were detected during the adipogenic differentiation of equine ASC in vitro. The equine APRIL gene was located on chromosome 11, spans 1852 base pairs (bp). Its open reading frame covers 753 bp, encoding a 250-amino acid protein with the typical TNF structure domain. During the two weeks' adipogenic differentiation of equine ASC, although the protein expression of APRIL and TACI had an insignificant change, that of BCMA increased significantly. Moreover, with the addition of recombinant protein His6-sAPRIL, a reduced differentiation of equine ASC toward adipocyte was detected. These results may provide the basis for investigating the role of APRIL in ASC adipogenic differentiation.
Publication Date: 2016-08-28 PubMed ID: 27565870DOI: 10.1080/10495398.2016.1182540Google Scholar: Lookup
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- Journal Article
Summary
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This study identified the equine A proliferation inducing ligand (APRIL) gene from adipose-derived stem cells (ASC) and tracked its protein expression during adipogenic differentiation. Results suggest that APRIL could possibly influence the differentiation of equine ASC.
Molecular Identification of Equine APRIL
- The first part of the study detailed the identification of Equine APRIL, a member of the Tumor Necrosis Factor (TNF) superfamily.
- Its gene was found on chromosome 11 and spans around 1852 base pairs.
- The open reading frame of the gene covered 753 base pairs, encoding 250 amino acids to form a protein with a typical structure of the TNF superfamily.
Expression Analysis of Equine APRIL during Adipogenic Differentiation
- Researchers then conducted an expression analysis of this equine APRIL gene during the adipogenic differentiation of equine ASC, a process that spans two weeks.
- They found that though the protein expression of APRIL and TACI (Transmembrane activator and CAML interactor, one of APRIL’s two receptors) did not significantly change during this process, the protein expression of BCMA (B-cell maturation antigen, another receptor of APRIL) increased significantly.
Additional Findings and Conclusions
- An important finding was that the addition of a recombinant protein His6-sAPRIL led to reduced differentiation of equine ASC towards adipocytes, hinting towards a potential role of APRIL protein in controlling adipogenic differentiation.
- The study thus provides valuable insights that might form the basis for future investigation into the function of APRIL in stem cell adipogenic differentiation
Cite This Article
APA
Wu H, Bi X, Cao F, Zhu C, Liu H, Song J, Ma L, Ma L, Zhang Y, Zhao D, Liu H, Xu X, Zhang S.
(2016).
Molecular Characterization of Equine APRIL and its Expression Analysis During the Adipogenic Differentiation of Equine Adipose-Derived Stem Cell In Vitro.
Anim Biotechnol, 27(4), 262-268.
https://doi.org/10.1080/10495398.2016.1182540 Publication
Researcher Affiliations
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- b Basic Medical College , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
- c College of Pharmacy , Nanjing University of Chinese Medicine , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
- a Jiangsu Key Laboratory for Molecular and Medical Biotechnology , Life Science College, Nanjing Normal University , Nanjing , P.R. China.
MeSH Terms
- Adipocytes / cytology
- Adipogenesis / physiology
- Animals
- Cell Differentiation / physiology
- Cell Line
- Horses
- Stem Cells / cytology
- Tumor Necrosis Factor Ligand Superfamily Member 13 / analysis
- Tumor Necrosis Factor Ligand Superfamily Member 13 / chemistry
- Tumor Necrosis Factor Ligand Superfamily Member 13 / genetics
- Tumor Necrosis Factor Ligand Superfamily Member 13 / metabolism
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