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Home / Equine Research Bank / J Clin Microbiol / Molecular characterizations of human and animal group a rota...
Journal of clinical microbiology2005; 43(2); 669-675; doi: 10.1128/JCM.43.2.669-675.2005

Molecular characterizations of human and animal group a rotaviruses in the Netherlands.

Abstract: To gain more insight into interspecies transmission of rotavirus group A, human and animal fecal samples were collected between 1997 and 2001 in The Netherlands. A total of 110 human stool samples were successfully P and G genotyped by reverse transcriptase PCR. All strains belonged to the main human rotavirus genotypes G1 to G4, G9, [P4], [P6], [P8], and [P9]. [P8]G1 was predominant, and 5.5% belonged to the G9 genotype. Eleven percent of all P[8] genotypes could be genotyped only by a recently published modified primer. Rotavirus-positive fecal samples from 28 calf herds were genotyped by DNA sequencing. Genotypes G6 and G10 predominated; G6 and G10 were detected in 22 (78.6%) and 16 (57.1%) of the rotavirus-positive calf herds, respectively. In 12 (42.9%) calf herds, we found mixed infections. Genotype G8 was not found. Genotype G6 bovine rotaviruses were divided into three clusters: UK-like, VMRI-29-like, and Hun4-like. DNA sequencing of a part of the VP7 gene was shown to be useful as a quick determination of uncommon or novel strains of which the genotyping cannot be done by genotyping PCR. Of equine strains, both VP4 and VP7 genes could be used for genotyping: two [P12]G3 and four [P12]G14 equine rotaviruses were determined. We did not find indications for rotavirus interspecies transmissions, although the recently published human G6-Hun4 is genetically related to our G6 bovine isolates. All bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.
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Publication Date: 2005-02-08 PubMed ID: 15695662PubMed Central: PMC548030DOI: 10.1128/JCM.43.2.669-675.2005Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research focuses on the study of interspecies transmission of group A rotavirus in both humans and animals in the Netherlands between 1997 to 2001. The prevalent genotypes and their distributions were identified in both the species using various DNA sequencing techniques.

Methods

  • A total of 110 human stool samples and rotavirus-positive fecal samples from 28 calf herds were collected for the study.
  • Reverse transcriptase PCR was used for P and G genotyping of the human stool samples.
  • For the calf samples, genotypes were identified using DNA sequencing.

Findings

  • In humans, all identified rotavirus strains belonged to common human genotypes including G1 to G4, G9, [P4], [P6], [P8], and [P9]. The [P8]G1 was found to be predominant.
  • In calf herds, genotypes G6 and G10 were predominantly found. The presence of G8 genotype was not registered.
  • There were instances of mixed infections in the calf herds, found in about 42.9% of the population.
  • Three clusters identified within the G6 bovine rotaviruses were: UK-like, VMRI-29-like, and Hun4-like.

Significance

  • The study found that genotyping through DNA sequencing of a part of the VP7 gene was effective in quickly determining uncommon or novel strains that couldn’t be genotyped by genotyping PCR.
  • Two [P12]G3 and four [P12]G14 genotypes were determined in equine rotaviruses.
  • The research did not find indications for rotavirus interspecies transmissions, but it was found that the recently published human G6-Hun4 genotype is genetically related to the G6 bovine strain.
  • All the identified bovine, porcine, and equine rotaviruses were within genotypes previously reported for these animal species.

Cite This Article

APA
van der Heide R, Koopmans MP, Shekary N, Houwers DJ, van Duynhoven YT, van der Poel WH. (2005). Molecular characterizations of human and animal group a rotaviruses in the Netherlands. J Clin Microbiol, 43(2), 669-675. https://doi.org/10.1128/JCM.43.2.669-675.2005

Publication

Journal of clinical microbiology
ISSN: 0095-1137
NlmUniqueID: 7505564
Country: United States
Language: English
Volume: 43
Issue: 2
Pages: 669-675

Researcher Affiliations

van der Heide, R
  • Microbiological Laboratory for Health Protection, Bilthoven, The Netherlands.
Koopmans, M P G
    Shekary, N
      Houwers, D J
        van Duynhoven, Y T H P
          van der Poel, W H M

            MeSH Terms

            • Animals
            • Antigens, Viral / genetics
            • Capsid Proteins / genetics
            • Cattle
            • Cattle Diseases / epidemiology
            • Cattle Diseases / virology
            • DNA, Viral / analysis
            • Genotype
            • Humans
            • Netherlands / epidemiology
            • Phylogeny
            • Rotavirus / classification
            • Rotavirus / genetics
            • Rotavirus Infections / epidemiology
            • Rotavirus Infections / veterinary
            • Rotavirus Infections / virology
            • Sequence Analysis, DNA
            • Swine
            • Swine Diseases / epidemiology
            • Swine Diseases / virology

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