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Veterinary sciences2023; 10(1); 49; doi: 10.3390/vetsci10010049

Molecular Features and Antimicrobial Susceptibilities of Streptococcus equi ssp. equi Isolates from Strangles Cases in Indonesia.

Abstract: Strangles, caused by ssp. (), is a highly infectious and frequent disease of equines worldwide. No data are available regarding the molecular epidemiology of strangles in Indonesia. This study aimed to characterize isolates obtained from suspected strangles cases in Indonesia in 2018. Isolates originated from seven diseased horses on four different farms located in three provinces of Indonesia. Whole genome sequences of these isolates were determined and used for typing, multilocus sequence typing (MLST), and core genome MLS typing (cgMLST). Genomes were also screened for known antimicrobial resistance genes and genes encoding for the recombinant antigens used in the commercial Strangvac subunit vaccine. All seven isolates from Indonesia belonged to ST179 and carried allele 166. Isolates differed from each other by only 2 to 14 cgSNPs and built an exclusive sub-cluster within the Bayesian Analysis of Population Structure (BAPS) cluster 2 (BAPS-2) of the cgMLST scheme. All isolates revealed predicted amino acid sequence identity to seven and high similarity to one of the eight antigen fragments contained in Strangvac. Furthermore, all isolates were susceptible to beta-lactam antibiotics penicillin G, ampicillin, and ceftiofur. Our data suggest that the horses from this study were affected by strains of the same novel sublineage within globally distributed BAPS-2 of . Nevertheless, penicillin G can be used as a first-choice antibiotic against these strains and Strangvac may also be protective against Indonesian strains.
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Publication Date: 2023-01-10 PubMed ID: 36669050PubMed Central: PMC9867300DOI: 10.3390/vetsci10010049Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article focuses on investigating the characteristics and anti-microbial susceptibilities of an infectious strain of Streptococcus equi ssp. equi (S. equi), found in strangles disease in horses. The analysis was aimed at various isolates captured from cases in Indonesia in 2018, using advanced techniques such as multi-locus sequence typing and whole genome sequencing.

Objective of the Research

  • The primary objective of the study was to explore the genetic and molecular features of S. equi isolates collected from suspected strangles cases in Indonesia in 2018.

Methodology

  • The isolates were collected from seven diseased horses located in three different provinces of Indonesia.
  • Whole genome sequencing of these isolates was carried out. This data was then utilised for multi-locus sequence typing (MLST) and core genome MLS typing (cgMLST).
  • The genomes were scrutinised for known antimicrobial resistance genes and other genes encoding recombinant antigens used in the commercial Strangvac subunit vaccine.

Key Findings

  • All seven S. equi isolates from Indonesia belonged to ST179 and carried seh allele 166.
  • The isolates only varied from each other by 2 to 14 cgSNPs, and created a distinct sub-cluster within the Bayesian Analysis of Population Structure (BAPS) cluster 2 (BAPS-2) of the cgMLST scheme.
  • A predicted amino acid sequence identity was established with seven of the eight antigen fragments contained in Strangvac.
  • All isolates were found to be susceptible to beta-lactam antibiotics including penicillin G, ampicillin, and ceftiofur.

Implications and Conclusion

  • The findings suggest that the diseased horses were affected by strains of the same novel sublineage within the globally distributed BAPS-2 of S. equi.
  • Penicillin G could be applied as the first choice of antibiotic against these strains.
  • The Strangvac vaccine was also considered potentially protective against Indonesian strains. This indicates a possible wider applicability of the vaccine, provided it is used in a timely manner.

Cite This Article

APA
Rotinsulu DA, Ewers C, Kerner K, Amrozi A, Soejoedono RD, Semmler T, Bauerfeind R. (2023). Molecular Features and Antimicrobial Susceptibilities of Streptococcus equi ssp. equi Isolates from Strangles Cases in Indonesia. Vet Sci, 10(1), 49. https://doi.org/10.3390/vetsci10010049

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 10
Issue: 1
PII: 49

Researcher Affiliations

Rotinsulu, Dordia Anindita
  • Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, Germany.
  • School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia.
Ewers, Christa
  • Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, Germany.
Kerner, Katharina
  • Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, Germany.
Amrozi, Amrozi
  • School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia.
Soejoedono, Retno Damayanti
  • School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor 16680, Indonesia.
Semmler, Torsten
  • NG-1 Microbial Genomics, Robert Koch Institute, 13353 Berlin, Germany.
Bauerfeind, Rolf
  • Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, 35392 Giessen, Germany.

Grant Funding

  • 57381412 / German Academic Exchange Service

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study, in the collection, analyses, or interpretation of data, in the writing of the manuscript, or in the decision to publish the results.

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Citations

This article has been cited 8 times.
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