Molecular identification of Cryptosporidium spp. and Giardia duodenalis in grazing horses from Xinjiang, China.
Abstract: A total of 262 fecal specimens collected from grazing horses at five locations in Xinjiang, China were examined by PCR for Cryptosporidium spp. and Giardia duodenalis. The Cryptosporidium and G. duodenalis infection rates were 2.7% and 1.5%, respectively. Seven Cryptosporidium-positive specimens were found in foals (16.3%), and four G. duodenalis-positive specimens were found in mares (2.5%). Sequence analyses of 18S rRNA and gp60 genes revealed that seven animals were positive for the subtype VIaA15G4 of Cryptosporidium horse genotype. G. duodenalis assemblages A and B were identified by molecular characterization of the 16S rRNA and tpi genes. This is the first report of Cryptosporidium horse genotype and G. duodenalis in grazing horses from China.
Copyright © 2015 Elsevier B.V. All rights reserved.
Publication Date: 2015-03-06 PubMed ID: 25794943DOI: 10.1016/j.vetpar.2015.02.030Google Scholar: Lookup
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- Journal Article
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- Non-U.S. Gov't
Summary
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This research study examined the presence of two pathogenic microorganisms, Cryptosporidium spp. and Giardia duodenalis, in grazing horses in Xinjiang, China using molecular identification methods. It also reported the first identification of the Cryptosporidium horse genotype and G. duodenalis in horses from China.
Research Methodology
- The researchers collected 262 fecal samples from horses at five different locations in Xinjiang, China. The horses were chosen randomly.
- Using Polymerase Chain Reaction (PCR), a method used in molecular science to amplify small segments of DNA, the sample were examined for the presence of Cryptosporidium spp. and Giardia duodenalis.
Findings: Detection of Cryptosporidium spp. and Giardia duodenalis
- Infection rates of Cryptosporidium and G. duodenalis in the horses were 2.7% and 1.5% respectively – indicating that the infections are not widespread among this population.
- The highest prevalence of Cryptosporidium was found in foals (young horses) at 16.3%, while mares (adult female horses) had a G. duodenalis infection rate of 2.5%.
Genetic Sequencing of the Pathogens
- The team then carried out genetic sequencing on the samples containing the Cryptosporidium and G. duodenalis.
- They investigated the 18S rRNA and gp60 genes of Cryptosporidium and found all seven positive samples belonged to the subgroup VIaA15G4, belonging to the Cryptosporidium horse genotype.
- G. duodenalis were identified by analyzing the 16S rRNA and tpi genes. Both assemblage A and assemblage B of the microorganism were found.
Significance and Conclusion
- This study is significant because it provides the first report of the Cryptosporidium horse genotype and the presence of G. duodenalis in grazing horses in China. This may have implication for disease surveillance and control measures in horse populations.
- Further research is needed to understand the potential impact of these pathogens on the health of horse populations as well as the risk of zoonotic transmission to humans.
Cite This Article
APA
Qi M, Zhou H, Wang H, Wang R, Xiao L, Arrowood MJ, Li J, Zhang L.
(2015).
Molecular identification of Cryptosporidium spp. and Giardia duodenalis in grazing horses from Xinjiang, China.
Vet Parasitol, 209(3-4), 169-172.
https://doi.org/10.1016/j.vetpar.2015.02.030 Publication
Researcher Affiliations
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
- Department of Animal Science, Henan Vocational College of Agriculture, Zhongmu 451450, Henan, PR China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
- Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
- Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China.
- College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, PR China; International Joint Research Laboratory for Zoonotic Diseases of Henan, Zhengzhou 450002, PR China. Electronic address: zhanglx8999@henau.edu.cn.
MeSH Terms
- Animals
- China / epidemiology
- Cryptosporidiosis / epidemiology
- Cryptosporidiosis / parasitology
- Cryptosporidium / classification
- Cryptosporidium / isolation & purification
- Feces / parasitology
- Giardia lamblia / isolation & purification
- Giardiasis / epidemiology
- Giardiasis / parasitology
- Horse Diseases / epidemiology
- Horse Diseases / parasitology
- Horses
Citations
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- Hailu AW, Degarege A, Adamu H, Costa D, Villier V, Mouhajir A, Favennec L, Razakandrainibe R, Petros B. Molecular characterization of Cryptosporidium spp. from humans in Ethiopia.. PLoS One 2021;16(6):e0253186.
- Jian Y, Zhang X, Li X, Schou C, Charalambidou I, Ma L, Karanis P. Occurrence of Cryptosporidium and Giardia in wild birds from Qinghai Lake on the Qinghai-Tibetan Plateau, China.. Parasitol Res 2021 Feb;120(2):615-628.
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