Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil.
Abstract: Over the last recent years, the number of Q fever cases have has increased throughout the world. An epidemiological investigation was performed in the area in which the first molecular documentation of Q fever in Brazil was previously reported. Methods: Indirect immunofluorescence assay (IFA) and PCR of Coxiella burnetii targeting the htpAB gene were performed in samples from 14 dogs (blood); 1 cat (blood); 10 goats (blood, milk, vaginal swab and anal swab); 3 sheep (blood); and 2 horses (blood). Results: Two dogs, two sheep and five goats were seroreactive. DNA was amplified from 6 milk and 2 blood samples from goats and from dogs, respectively. The sequence of the amplicons exhibited 99% sequence similarity with the homologous sequence of the htpAB gene of C. burnetii RSA 331 (GenBank - CP000890). Conclusions: The results confirm C. burnetii infection in animals in Rio de Janeiro and reinforce the need for the surveillance of Q fever in Brazil.
Publication Date: 2014-05-28 PubMed ID: 24861300DOI: 10.1590/0037-8682-0076-2013Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Animal Health
- Diagnosis
- Disease control
- Disease Diagnosis
- Disease Prevalence
- Disease Surveillance
- Disease Treatment
- DNA
- Domestic Animals
- Epidemiology
- Equine Health
- Immunofluorescence Assay
- Infectious Disease
- Molecular biology
- Polymerase Chain Reaction
- Public Health
- Serology
- Veterinary Medicine
- Veterinary Research
- Veterinary Science
- Zoonotic Diseases
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
This research study investigated the occurrence of Q fever, caused by the bacterium Coxiella burnetii, in various domestic animals in the state of Rio de Janeiro, Brazil. The study found evidence of the infection in dogs, goats and sheep, underscoring the need to monitor for Q fever in Brazil.
Methodology
- The study was conducted in the same region where Q fever was first molecularly documented in Brazil.
- The researchers collected samples from various domestic animals, including 14 dogs, one cat, 10 goats, three sheep and two horses. The samples included blood, milk, vaginal swabs and anal swabs.
- The samples were tested for the presence of Coxiella burnetii using two techniques: Indirect immunofluorescence assay (IFA), which detects antibodies produced by the body in response to the infection, and PCR (Polymerase chain reaction), a technique used to amplify and hence detect the DNA of the bacterium.
Results
- Two dogs, two sheep and five goats tested positive for Q fever antibodies, suggesting they had been exposed to the infection.
- DNA of Coxiella burnetii was found in six milk and two blood samples taken from the goats and dogs respectively, confirming the presence of the infection in these animals.
- The DNA sequence of the bacteria in the samples showed a 99% similarity to the htpAB gene of C. burnetii RSA 331, a known strain of the bacterium available in a global genes archive (GenBank – CP000890).
Conclusions
- The findings provide confirmation of the C. burnetii infection in the tested animals in the region of Rio de Janeiro.
- These results emphasize the importance of continued surveillance and monitoring for Q fever in Brazil, particularly in light of the increase in reported Q fever cases worldwide in recent years.
Cite This Article
APA
Mares-Guia MA, Rozental T, Guterres A, Gomes R, Almeida DN, Moreira NS, Barreira JD, Favacho AR, Santana AL, Lemos ER.
(2014).
Molecular identification of the agent of Q fever – Coxiella burnetii – in domestic animals in State of Rio de Janeiro, Brazil.
Rev Soc Bras Med Trop, 47(2), 231-234.
https://doi.org/10.1590/0037-8682-0076-2013 Publication
Researcher Affiliations
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
- Secretaria de Saúde do Município de Itaboraí, Itaborarí, RJ.
- Laboratório de Hantaviroses e Rickettsioses, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
MeSH Terms
- Animals
- Animals, Domestic / microbiology
- Brazil
- Cats
- Coxiella burnetii / genetics
- Dogs
- Female
- Fluorescent Antibody Technique, Indirect
- Goats
- Horses
- Polymerase Chain Reaction
- Q Fever / diagnosis
- Q Fever / microbiology
- Q Fever / veterinary
- Sheep
Citations
This article has been cited 12 times.- Anastácio S, Anjos S, Neves S, Neves T, Esteves P, Craveiro H, Madeira B, Pires MDA, Sousa S, da Silva G, Vilhena H. Coxiella burnetii in Dogs and Cats from Portugal: Serological and Molecular Analysis.. Pathogens 2022 Dec 13;11(12).
- Noguera Zayas LP, Rüegg S, Torgerson P. The burden of zoonoses in Paraguay: A systematic review.. PLoS Negl Trop Dis 2021 Nov;15(11):e0009909.
- Fontes SDS, Maia FM, Ataides LS, Conte FP, Lima-Junior JDC, Rozental T, da Silva Assis MR, Júnior AAP, Fernandes J, de Lemos ERS, Rodrigues-da-Silva RN. Identification of Immunogenic Linear B-Cell Epitopes in C. burnetii Outer Membrane Proteins Using Immunoinformatics Approaches Reveals Potential Targets of Persistent Infections.. Pathogens 2021 Sep 28;10(10).
- Mahlobo-Shwabede SIC, Zishiri OT, Thekisoe OMM, Makalo MJR. Molecular Detection of Coxiella burnetii, Rickettsia africae and Anaplasma Species in Ticks from Domestic Animals in Lesotho.. Pathogens 2021 Sep 14;10(9).
- Rabaza A, Macías-Rioseco M, Fraga M, Uzal FA, Eisler MC, Riet-Correa F, Giannitti F. Coxiella burnetii abortion in a dairy farm selling artisanal cheese directly to consumers and review of Q fever as a bovine abortifacient in South America and a human milk-borne disease.. Braz J Microbiol 2021 Dec;52(4):2511-2520.
- Oliveira VDC, Junior AAVM, Ferreira LC, Calvet TMQ, Dos Santos SA, Figueiredo FB, Campos MP, Rodrigues FDCC, de Oliveira RVC, de Lemos ERS, Rozental T, da Silva RG, Amendoeira MRR, Teles-de-Freitas R, Bruno RV, Morgado FN, Miranda LFC, Menezes RC. Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum.. PLoS One 2021;16(3):e0247560.
- Rozental T, Faria LS, Forneas D, Guterres A, Ribeiro JB, Araújo FR, Lemos ERS, Silva MR. First molecular detection of Coxiella burnetii in Brazilian artisanal cheese: a neglected food safety hazard in ready-to-eat raw-milk product.. Braz J Infect Dis 2020 May-Jun;24(3):208-212.
- Macías-Rioseco M, Riet-Correa F, Miller MM, Sondgeroth K, Fraga M, Silveira C, Uzal FA, Giannitti F. Bovine abortion caused by Coxiella burnetii: report of a cluster of cases in Uruguay and review of the literature.. J Vet Diagn Invest 2019 Jul;31(4):634-639.
- Ferreira MS, Guterres A, Rozental T, Novaes RLM, Vilar EM, Oliveira RC, Fernandes J, Forneas D, Junior AA, Brandão ML, Cordeiro JLP, Del Valle Alvarez MR, Althoff SL, Moratelli R, Cordeiro-Estrela P, Silva RCD, Lemos ERS. Coxiella and Bartonella spp. in bats (Chiroptera) captured in the Brazilian Atlantic Forest biome.. BMC Vet Res 2018 Sep 10;14(1):279.
- Mares-Guia MAMM, Guterres A, Rozental T, Ferreira MDS, Lemos ERS. Clinical and epidemiological use of nested PCR targeting the repetitive element IS1111 associated with the transposase gene from Coxiella burnetii.. Braz J Microbiol 2018 Jan-Mar;49(1):138-143.
- Pradeep J, Stephen S, Pooja P, Akshayavardhini A, Sangeetha B, Antony PX. Coxiellosis in domestic livestock of Puducherry and Tamil Nadu: Detection of Coxiella burnetii DNA by polymerase chain reaction in slaughtered ruminants.. Vet World 2017 Jun;10(6):667-671.
- Mares-Guia MA, Rozental T, Guterres A, Ferreira Mdos S, Botticini Rde G, Terra AK, Marraschi S, Bochner R, Lemos ER. Molecular Identification of Q Fever in Patients with a Suspected Diagnosis of Dengue in Brazil in 2013-2014.. Am J Trop Med Hyg 2016 May 4;94(5):1090-4.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
