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Veterinary sciences2021; 8(11); doi: 10.3390/vetsci8110282

Molecular Prevalence of Equine Parvovirus-Hepatitis in the Sera of Clinically Healthy Horses in South Korea.

Abstract: Equine parvovirus-hepatitis (EqPV-H) causes equine hepatitis. The prevalence of EqPV-H in healthy horses has been reported in the United States, China, Germany, and Austria. The present study determined the prevalence of EqPV-H in the sera of clinically healthy horses in South Korea to identify the potential factors for infection and examine the genetic diversity of EqPV-H DNA sequences through comparison with foreign strains. Serum samples collected from 321 horses were tested for EqPV-H using non-structural protein 1 (NS1)-specific polymerase chain reaction. The associations of EqPV-H infection with sex, age, aspartate aminotransferase and γ-glutamyl transferase levels, and race performance were analyzed. Fourteen samples tested positive for EqPV-H (4.4%, 14/321), and EqPV-H infection was associated with sex (p = 0.006) and performance (p = 0.049). In both EqPV-H-positive and control horses, liver-specific biochemical analytes were within the normal ranges. Phylogenetic analyses based on the partial sequences of EqPV-H NS1 revealed that the Korean EqPV-H isolates shared approximately 98.7-100% similarity. Of these, 11 Korean isolates shared high similarity with strains from the United States, Germany, and China, and the remaining three strains were distinct in phylogenetic analyses. The present study describes the current molecular prevalence, potential risk factors, and genetic diversity of Korean EqPV-H.
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Publication Date: 2021-11-19 PubMed ID: 34822655PubMed Central: PMC8619122DOI: 10.3390/vetsci8110282Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research study explores the prevalence of Equine parvovirus-hepatitis (EqPV-H), a cause of equine hepatitis, in healthy horses in South Korea and examines the genetic diversity of EqPV-H DNA sequences.

Study Methodology

  • The researchers collected serum samples from 321 horses which were clinically healthy.
  • These samples were tested for the presence of EqPV-H, using a test called ‘non-structural protein 1 (NS1)-specific polymerase chain reaction’.
  • They analyzed whether EqPV-H infection was related to the horse’s sex, age, aspartate aminotransferase and γ-glutamyl transferase levels, and the horse’s race performance.

Study Findings

  • 14 out of 321 samples, or roughly 4.4%, tested positive for EqPV-H.
  • There was a significant association found between EqPV-H infection and horse’s sex and performance.
  • Despite presence of EqPV-H, the liver-specific biochemical analytes were within the normal ranges in both EqPV-H positive and control horses, meaning the healthy horses were able to maintain normal liver function.

Genetic Diversity of EqPV-H

  • The researchers also analyzed the genetic diversity of the detected EqPV-H, by analyzing the partial sequences of EqPV-H NS1 – a nonstructural protein.
  • The Korean EqPV-H isolates shared approximately 98.7-100% similarity.
  • Out of all the detected Korean isolates, 11 were found to have high similarity with strains from the United States, Germany, and China. However, three strains were different as per the phylogenetic analyses.

The key takeaway from this study is that it conducted an investigation of EqPV-H prevalence in horses in South Korea, its potential risk factors and genetic similarity with strains from other countries. As EqPV-H can cause equine hepatitis, an understanding of its prevalence and genetic diversity can help initiate measures for disease control and improve treatment strategies.

Cite This Article

APA
Lee SK, Park D, Lee I. (2021). Molecular Prevalence of Equine Parvovirus-Hepatitis in the Sera of Clinically Healthy Horses in South Korea. Vet Sci, 8(11). https://doi.org/10.3390/vetsci8110282

Publication

Veterinary sciences
ISSN: 2306-7381
NlmUniqueID: 101680127
Country: Switzerland
Language: English
Volume: 8
Issue: 11

Researcher Affiliations

Lee, Sang-Kyu
  • Veterinary Centre, Korea Racing Authority, Gwacheon 13822, Korea.
Park, Dongsun
  • Department of Biology Education, Korea National University of Education, Cheongju 28173, Korea.
Lee, Inhyung
  • College of Veterinary Medicine, Seoul National University, Seoul 08826, Korea.

Conflict of Interest Statement

The authors declare no conflict of interest.

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Citations

This article has been cited 5 times.
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