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Archives of Razi Institute2024; 79(2); 327-334; doi: 10.32592/ARI.2024.79.2.327

Molecular Study of Anaplasma spp. in Horses, Sheep, and Goats with Phylogenetic Analysis in Northwest Iran.

Abstract: Anaplasmosis, a tick-borne disease with worldwide distribution, impacts ruminants, equines, carnivores, and humans. This study aimed to investigate Anaplasma phagocytophilum in horses from Ardabil province and Anaplasma ovis in small ruminants from East Azerbaijan province using the Nested Polymerase Chain Reaction (PCR) method. Blood samples were taken from the jugular vein of 100 healthy horses in the Ardabil province and 156 healthy sheep and goats (116 sheep and 40 goats) in the East Azerbaijan province during the spring and summer seasons of 2016 in northwest Iran. The collected blood samples were stored at -20°C until the molecular experiments were conducted. Nested PCR was employed to detect A. phagocytophilum in horses and A. ovis in small ruminants using extracted DNA and amplifying 16S rRNA and msp4 genes. The Chi-square test of independence was used to determine the relationship between Anaplasma spp., infection, and independent variables, including age, gender, animal species, and sampling location. None of the 100 samples collected from horses in the Ardabil province were positive for A. phagocytophilum. In the East Azerbaijan province, 11 out of the 156 (7.05%) blood samples collected from sheep and goats tested positive for A. ovis. In addition, A. ovis infection was not significantly related to the independent variables. Phylogenetic analysis showed that the sequence obtained in this study (MH790273) had 100% homology with the sequence obtained from sheep infected with Anaplasma in Ahvaz province (JQ621903.1). The findings of this study can contribute to the prevention and control of anaplasmosis in farm animals in northwestern Iran.
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Publication Date: 2024-04-30 PubMed ID: 39463709PubMed Central: PMC11512185DOI: 10.32592/ARI.2024.79.2.327Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study investigates the prevalence of the tick-borne disease Anaplasmosis in horses, sheep, and goats in two provinces of northwest Iran using a method called Nested Polymerase Chain Reaction (PCR). The research demonstrated that none of the studied horses were carriers, but small ruminants showed a low-level presence of the disease.

Methodology

  • The researchers collected blood samples from the jugular vein of 100 healthy horses in Ardabil province and 156 healthy sheep and goats in East Azerbaijan province during the spring and summer seasons of 2016.
  • The blood samples were stored at -20°C until needed for molecular experimentation.
  • Nested PCR, a method of DNA amplification, was used to detect the presence of Anaplasma spp. (the bacteria that causes Anaplasmosis) in the animals’ blood.
  • The researchers aimed to amplify two specific genes, pCS20 and msp4, which are associated with Anaplasma bacteria.

Results and Analysis

  • None of the 100 horse samples showed the presence of Anaplasma spp.
  • In contrast, 7.05% of blood samples, totaling 11 out of 156, from sheep and goats in East Azerbaijan province tested positive for Anaplasma spp.
  • The presence or absence of the bacteria did not show a significant connection with variables such as age, gender, animal species, or sampling location.

Phylogenetic Analysis

  • Researchers employed phylogenetic analysis to trace the genetic lineage of the discovered bacteria strains.
  • The sequence obtained from this study (MH790273) matched 100% with a previous sequence obtained from sheep infected with Anaplasma in Ahvaz province (JQ621903.1).

Conclusion

  • The research provided valuable data about the incidence of Anaplasma spp. in domesticated animals.
  • Though the disease prevalence was low among the tested animals and nonexistent in horses, it could still have implications for Anaplasmosis disease control and prevention in northwest Iran.

Cite This Article

APA
Akbari H, Basaki M, Imani Baran A, Akbarzadeh Z. (2024). Molecular Study of Anaplasma spp. in Horses, Sheep, and Goats with Phylogenetic Analysis in Northwest Iran. Arch Razi Inst, 79(2), 327-334. https://doi.org/10.32592/ARI.2024.79.2.327

Publication

Archives of Razi Institute
ISSN: 2008-9872
NlmUniqueID: 101549567
Country: Iran
Language: English
Volume: 79
Issue: 2
Pages: 327-334

Researcher Affiliations

Akbari, H
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Basaki, M
  • Department of Basic Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Imani Baran, A
  • Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
Akbarzadeh, Z
  • Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.

MeSH Terms

  • Animals
  • Iran / epidemiology
  • Goats
  • Sheep
  • Goat Diseases / microbiology
  • Goat Diseases / epidemiology
  • Sheep Diseases / microbiology
  • Sheep Diseases / epidemiology
  • Anaplasmosis / epidemiology
  • Anaplasmosis / microbiology
  • Phylogeny
  • Horses
  • RNA, Ribosomal, 16S / analysis
  • Anaplasma ovis / genetics
  • Anaplasma ovis / isolation & purification
  • Horse Diseases / microbiology
  • Horse Diseases / epidemiology
  • Male
  • Female
  • Anaplasma phagocytophilum / genetics
  • Anaplasma phagocytophilum / isolation & purification
  • Polymerase Chain Reaction / veterinary
  • Anaplasma / genetics
  • Anaplasma / isolation & purification
  • Sheep, Domestic

Conflict of Interest Statement

The authors declared no conflict of interest.

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This article includes 25 references
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