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Home / Equine Research Bank / Transbound Emerg Dis / Molecular surveillance of equine parvovirus-hepatitis from o...
Transboundary and emerging diseases2022; 69(6); e3455-e3461; doi: 10.1111/tbed.14746

Molecular surveillance of equine parvovirus-hepatitis from oral, nasal, vaginal, and semen specimens collected from horses living in Korea.

Abstract: Equine parvovirus-hepatitis (EqPV-H) is one of the etiological agents of Theiler's disease, causing fulminant hepatitis; however, its transmission route and pathogenesis remain unclear. In the present study, we aimed to determine EqPV-H shedding in oral/nasal/vaginal swabs or semen samples from horses living in Korea using nested polymerase chain reaction. We then used the data obtained to investigate various risk factors associated with EqPV-H including viral shedding, hepatopathological changes, and genetic diversity. Our data revealed occurrence of EqPV-H shedding in these animals (oral: 3/102 [2.9%]; nasal: 3/102 [2.9%]; semen: 1/9 [11.1%]) and identified that both age and country of foaling were significantly associated with EqPV-H shedding (p < .05). In addition, we noted that one of the newly isolated strains clustered separately from the other strains in the phylogenetic tree, revealing unique nucleotide and amino acid substitutions. This is a field surveillance study providing evidence of natural and venereal shedding of EqPV-H and describing its presence in both oral/nasal fluids and semen. This epidemiological and clinical analysis may help specify the clinicopathological features of EqPV-H and facilitate the development of novel disease prevention strategies.
© 2022 Wiley-VCH GmbH.
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Publication Date: 2022-11-07 PubMed ID: 36271506DOI: 10.1111/tbed.14746Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research investigates how equine parvovirus-hepatitis (EqPV-H) is shed or transmitted in horses living in Korea thorough various bodily fluids, and the associated risk factors. The study provides field-based evidence of EqPV-H being transmitted through natural and sexual activities, which helps to understand the disease and develop prevention strategies.

Objective of the Study

  • The main aim of the study was to determine the shedding of EqPV-H in horses through oral, nasal, vaginal swabs or semen samples.
  • Another goal of the study was to investigate and identify the potential risk factors associated with EqPV-H shedding, hepatopathological changes, and genetic diversity of the virus.

Methodology

  • The researchers collected samples of oral, nasal, vaginal swab or semen from horses living in Korea.
  • These samples were then tested for EqPV-H using the nested polymerase chain reaction (PCR), a highly sensitive method which detects the DNA of the virus.

Findings

  • The data showed evidence of EqPV-H shedding in horses, with percentages varying based on the type of sample (oral, nasal, and semen).
  • The study found that both the age of the horses and their country of birth were significantly associated with EqPV-H shedding.
  • Interestingly, one of the new strains of the virus, when isolated, was found to stand apart from the others in the phylogenetic tree analysis, indicating some unique nucleotide and amino acid substitutions.

Impact of the Study

  • The study provides crucial data on how the hepatitis virus spreads in horses, an area which was previously unclear.
  • The investigation of EqPV-H’s shedding from various bodily fluids contributes to a better understanding of its transmission routes.
  • By noting the associated risk factors, it leads to further research in mitigating these risks, thereby preventing or reducing the spread of the illness.

Final Remarks

  • This study contributes significantly to the field of veterinary medicine, particularly in the control and prevention of EqPV-H.
  • It provides an essential understanding of the shedding patterns of the virus which helps in planning strategic and international health management for horses.
  • The study can help guide the development of novel disease prevention strategies and therapies.

Cite This Article

APA
Yoon J, Park T, Kim A, Park J, Park BJ, Ahn HS, Go HJ, Kim DH, Lee JB, Park SY, Song CS, Lee SW, Choi IS. (2022). Molecular surveillance of equine parvovirus-hepatitis from oral, nasal, vaginal, and semen specimens collected from horses living in Korea. Transbound Emerg Dis, 69(6), e3455-e3461. https://doi.org/10.1111/tbed.14746

Publication

Transboundary and emerging diseases
ISSN: 1865-1682
NlmUniqueID: 101319538
Country: Germany
Language: English
Volume: 69
Issue: 6
Pages: e3455-e3461

Researcher Affiliations

Yoon, Jungho
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju-si, Korea.
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Park, Taemook
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju-si, Korea.
Kim, Ahram
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju-si, Korea.
Park, Jongyoung
  • Equine Clinic, Jeju Stud Farm, Korea Racing Authority, Jeju-si, Korea.
Park, Byung-Joo
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Ahn, Hee-Seop
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Go, Hyeon-Jeong
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Kim, Dong-Hwi
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Lee, Joong-Bok
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Park, Seung-Yong
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Song, Chang-Seon
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Lee, Sang-Won
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
Choi, In-Soo
  • Department of Infectious Diseases, College of Veterinary Medicine, Konkuk University, Seoul, Korea.
  • KU Center for Animal Blood Medical Science, Konkuk University, Seoul, Korea.

MeSH Terms

  • Female
  • Animals
  • Horses
  • Parvovirus
  • Parvoviridae Infections / veterinary
  • Phylogeny
  • Semen
  • Hepatitis, Viral, Animal / epidemiology
  • Horse Diseases / epidemiology
  • Horse Diseases / pathology
  • Parvovirinae
  • Hepatitis
  • Republic of Korea

Grant Funding

  • 320005-04-3-SB0a0 / Ministry of Agriculture, Food and Rural Affairs

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