Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.
Abstract: To confirm the positive conversion of antibodies against erythrocyte antigens in horses, possible blood transfusion donor horses selected from draft horse populations were periodically monitored with an indirect antiglobulin (Coombs) test for approximately 3 years. In this study, 19 horses (16 females and 3 males) were investigated, and five mares showed alloantibodies during the monitoring period. Four mares were typically pregnant when positive conversion was detected, whereas no particular cause of conversion could be observed for one mare based on its clinical records. In the analyzed horses, most positive conversions were possibly due to pregnancy, as conversion occurred more often during this period than after parturition. Pregnancy is considered a key event for positive conversion. Additionally, in cases in which unknown causative sensitization is confirmed, continuous monitoring with a test to detect antibodies should be performed, even if the possible donor is selected and maintained.
©2023 The Japanese Society of Equine Science.
Publication Date: 2023-06-12 PubMed ID: 37405070PubMed Central: PMC10315640DOI: 10.1294/jes.34.47Google Scholar: Lookup
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Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research involved monitoring potential equine blood donors for signs of developing antibodies against erythrocyte antigens. The primary finding is that pregnancy seems to contribute significantly to this occurrence.
Objective of the study
- The research intended to verify the formation of antibodies against erythrocyte antigens in horses, especially those potential for blood transfusion work.
- This was accomplished by monitoring a selection of draft horses over roughly 3 years using an indirect antiglobulin test or Coombs test.
Research methodology
- The study involved 19 horses comprising 16 females and 3 males.
- Throughout the period, these horses went under close monitoring with an objective to notice the presence of alloantibodies.
- The use of Coombs test, known for detecting the antibodies that might be harming the red blood cells (RBCs), was a key technique in this research.
Findings of the study
- Out of the total selected horses, five mares were found with alloantibodies during the monitoring period.
- Of these five mares, four were pregnant when the researchers detected the positive conversion of antibodies, hinting at a possible link between pregnancy and antibody conversion.
- For one mare with a positive conversion, the researchers could not establish an identifiable cause, as per the clinical records.
- Pregnancy was identified as a pivotal event contributing to this positive antibody conversion, as the conversion frequency was higher during pregnancy than after childbirth.
Implications of the study
- The findings suggest that for optimal selection and maintenance of potential blood donor horses, continuous monitoring for antibodies is crucial even if the triggering factor is unknown.
- The association between pregnancy and positive antibody conversion is an important consideration for blood transfusion programs in horses.
Cite This Article
APA
Kakoi H, Kikuchi M, Ishige T, Hirosawa Y, Tanaka S, Nagata SI.
(2023).
Monitoring the positive conversion of anti-erythrocyte antibodies in blood transfusion donor horses.
J Equine Sci, 34(2), 47-49.
https://doi.org/10.1294/jes.34.47 Publication
Researcher Affiliations
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
- National Livestock Breeding Center Tokachi Station, Hokkaido 080-0572, Japan.
- National Livestock Breeding Center Tokachi Station, Hokkaido 080-0572, Japan.
- Laboratory of Racing Chemistry, Tochigi 320-0851, Japan.
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