Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry.
Abstract: Serotyping is an important element for surveillance of Salmonella. In this study, an anti-O:4 Salmonella monoclonal antibody-based competitive enzyme-linked immunosorbent assay that could identify Salmonella infection in cow, pig, horse, and chicken was developed. This detection system can therefore be useful for a wide range of animals and for humans.
Copyright © 2014 Elsevier B.V. All rights reserved.
Publication Date: 2014-11-13 PubMed ID: 25447889DOI: 10.1016/j.mimet.2014.11.002Google Scholar: Lookup
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- Evaluation Study
- Journal Article
- Research Support
- Non-U.S. Gov't
- Animal Health
- Animal Science
- Antibodies
- Disease Surveillance
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Epidemiology
- Equine Health
- Food Safety
- Infectious Disease
- Livestock
- Monoclonal Antibodies
- Public Health
- Salmonellosis
- Serodiagnosis
- Seroprevalence
- Serotypes
- Veterinary Medicine
- Veterinary Research
- Veterinary Science
Summary
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This research paper discusses the development of a monoclonal antibody-based test that can detect the presence of Salmonella bacteria in various livestock and poultry animals.
Objective of the Research
- The main goal of this study was to develop a test using monoclonal antibodies, which can help in identifying the presence of the O:4 Salmonella bacteria in various animals such as cows, pigs, horses and chickens.
Importance of Serotyping
- Serotyping is a process that identifies bacteria based on the types of antigens present on their cell surfaces.
- In the context of Salmonella bacteria, serotyping is crucial for the successful surveillance and control of the bacteria’s spread in animal populations and to humans.
Development of the Assay
- The researchers developed a competitive enzyme-linked immunosorbent assay (ELISA) which is a common laboratory technique used for measuring antigens (foreign substances that induce an immune response) in biological samples.
- Specifically, they incorporated a monoclonal antibody against O:4 Salmonella into the assay. Monoclonal antibodies are laboratory-made molecules designed to mimic the immune system’s response to specific pathogens, in this case, the O:4 Salmonella bacteria.
Utility of the Test
- This developed assay could potentially be very useful as it can be applied to a wide range of animals. It allows for rapid detection and identification of O:4 Salmonella, thereby enhancing efforts to monitor and control the spread of this bacteria in livestock and poultry populations, and ultimately, to humans.
Cite This Article
APA
Aribam SD, Ogawa Y, Matsui H, Hirota J, Okamura M, Akiba M, Shimoji Y, Eguchi M.
(2014).
Monoclonal antibody-based competitive enzyme-linked immunosorbent assay to detect antibodies to O:4 Salmonella in the sera of livestock and poultry.
J Microbiol Methods, 108, 1-3.
https://doi.org/10.1016/j.mimet.2014.11.002 Publication
Researcher Affiliations
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
- Kitasato Institute for Life Sciences and Graduate School of Control Sciences, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan.
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
- Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine, Towada, Aomori 034-8628, Japan.
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan.
- Bacterial and Parasitic Disease Research Division, National Institute of Animal Health, NARO, Tsukuba, Ibaraki 305-0856, Japan. Electronic address: egumaro@affrc.go.jp.
MeSH Terms
- Animals
- Antibodies, Bacterial / analysis
- Antibodies, Monoclonal / analysis
- Cattle
- Chickens
- Enzyme-Linked Immunosorbent Assay / instrumentation
- Enzyme-Linked Immunosorbent Assay / methods
- Horses
- Livestock / blood
- Poultry Diseases / blood
- Poultry Diseases / microbiology
- Salmonella / immunology
- Salmonella / isolation & purification
- Salmonella Infections, Animal / blood
- Salmonella Infections, Animal / microbiology
- Swine
Citations
This article has been cited 2 times.- Zhang L, Du X, Chen Z, Chen C, Gong N, Song Y, Song Y, Han Q, Xia X, Luo H, Zhang J. Instrument-Free and Visual Detection of Salmonella Based on Magnetic Nanoparticles and an Antibody Probe Immunosensor. Int J Mol Sci 2019 Sep 19;20(18).
- Li Q, Zhu Y, Yin K, Xu L, Yin C, Li Y, Ren J, Yuan Y, Jiao X. Purification of recombinant IpaJ to develop an indirect ELISA-based method for detecting Salmonella enterica serovar Pullorum infections in chickens. BMC Vet Res 2019 Jan 3;15(1):3.
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