Morphology of the nerve endings in laryngeal mucosa of the horse.
Abstract: To discuss the significance of laryngeal sensation on various disorders of the horse, we studied the morphological and topographical characteristics of sensory structures in the laryngeal mucosa using immunohistochemistry and immunoelectron microscopy. Various sensory structures, i.e. glomerular endings, taste buds and intraepithelial free nerve endings, were found in the laryngeal mucosa by immunohistochemistry for protein gene product 9.5 (PGP 9.5) and neurofilament 200kD (NF200). Glomerular nerve endings were distributed mainly in the epiglottic mucosa; some endings were also found in the arytenoid region arising from thick nerve fibres running through the subepithelial connective tissue. Some terminals directly contacted the epithelial cells. Taste buds were distributed in the epithelium of the epiglottis and aryepiglottic fold. In the whole mount preparation, the taste buds were supplied by the terminal branching of the thick nerve fibres. In some cases, the taste buds were arranged around the opening of the duct of the epiglottic glands. The intraepithelial free nerve endings were found to be immunoreactive for substance P (SP) and calcitonin gene-related peptide (CGRP). These nerve endings were surrounded by the polygonal stratified epithelial cells in the supraglottic region, and by the ciliated cells in the subglottic region. The density of the intraepithelial free nerve endings was highest in the corniculate process of the arytenoid region and lowest in the vocal cord mucosa. The densities of CGRP- and SP-immunoreactive nerve endings in the arytenoid region were (mean +/- s.d.) 30.6+/-12.0 and 10.0+/-4.9 per unit epithelial length (1 mm), respectively and in the vocal fold mucosa, 1.1+/-0.9 and 0.8+/-0.7, respectively. Approximately one half of the CGRP immunoreactive nerve endings were immunoreactive for SP, and most SP-immunoreactive nerve endings were also immunoreactive for CGRP. Well-developed subepithelial plexus with numerous intraepithelial fibres were observed in flat or round mucosal projections that existed on the corniculate process of the arytenoid region. In conclusion, the laryngeal mucosa of the horse seems to have morphology- and/or location-dependent sensory mechanisms against various endo-and exogenious stimuli.
Publication Date: 2001-03-27 PubMed ID: 11266064DOI: 10.1111/j.2042-3306.2001.tb00593.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article explores the morphological structures and locations of sensory nerve endings in the laryngeal mucosa of the horse to gain insight into the effect of laryngeal sensation on various horse disorders.
Methodology
- The study used immunohistochemistry and immunoelectron microscopy to study the sensory structures present in the horse’s laryngeal mucosa.
- The sensory structures they were concerned with included the glomerular endings, taste buds, and intraepithelial free nerve endings.
- They identified these structures by looking for the presence of protein gene product 9.5 (PGP 9.5) and neurofilament 200kD (NF200), which are proteins known to be present in nerve tissue.
Results
- Golmerular nerve endings were primarily discovered in the epiglottic mucosa, although some were found in the arytenode region.
- The arytenoid region’s nerve endings appeared to originate from thick nerve fibers flowing through connective tissue beneath the epithelium.
- Taste buds were distributed in the epithelium of the aryepiglottic fold and the epiglottis, often in proximity to the openings of the ducts of the epiglottic glands.
- Intraepithelial free nerve endings, detected due to their reactivity to substance P (SP) and calcitonin gene-related peptide (CGRP), were found enveloped by supraglottic region’s polygonal stratified epithelial cells and subglottic region’s ciliated cells.
- The highest density of these intraepithelial free nerve endings was in the corniculate process of the arytenoid region while the lowest was in the vocal cord mucosa.
- The densities of CGRP- and SP-immunoreactive nerve endings in different regions of the larynx were also calculated and reported.
- It was observed that about half of the CGRP reactive nerve endings were also reactive for SP, and most SP active nerve endings were reactive for CGRP.
- A well-developed subepithelial plexus with numerous intraepithelial fibers was noticed in flat or round mucosal projections on the corniculate process of the arytenoid region.
Conclusion
- The study concluded that the morphology and location of the sensory mechanisms in a horse’s laryngeal mucosa vary and may play a role in responding to different endogenous and exogenous stimuli.
Cite This Article
APA
Yamamoto Y, Atoji Y, Hobo S, Yoshihara T, Suzuki Y.
(2001).
Morphology of the nerve endings in laryngeal mucosa of the horse.
Equine Vet J, 33(2), 150-158.
https://doi.org/10.1111/j.2042-3306.2001.tb00593.x Publication
Researcher Affiliations
- Department of Veterinary Science, Faculty of Agriculture, Gifu University, Japan.
MeSH Terms
- Animals
- Female
- Horses / anatomy & histology
- Immunohistochemistry / veterinary
- Larynx / anatomy & histology
- Larynx / ultrastructure
- Male
- Mucous Membrane / innervation
- Sensory Receptor Cells / anatomy & histology
Citations
This article has been cited 5 times.- Pilmane M, Sumerags D, Jain N, Jain S, Sumeraga G. Singer's Nodules: Investigating the Etiopathogenetic Markers Progressing Their Pathogenesis and Clinical Manifestations.. Biology (Basel) 2021 Dec 3;10(12).
- Foote AG, Thibeault SL. Sensory Innervation of the Larynx and the Search for Mucosal Mechanoreceptors.. J Speech Lang Hear Res 2021 Feb 17;64(2):371-391.
- Sasaki R, Sato T, Yajima T, Kano M, Suzuki T, Ichikawa H. The distribution of TRPV1 and TRPV2 in the rat pharynx.. Cell Mol Neurobiol 2013 Jul;33(5):707-14.
- Sato T, Fujita M, Kano M, Hosokawa H, Kondo T, Suzuki T, Kasahara E, Shoji N, Sasano T, Ichikawa H. The distribution of transient receptor potential melastatin-8 in the rat soft palate, epiglottis, and pharynx.. Cell Mol Neurobiol 2013 Mar;33(2):161-5.
- Soda Y, Yamamoto Y. Morphology and chemical characteristics of subepithelial laminar nerve endings in the rat epiglottic mucosa.. Histochem Cell Biol 2012 Jul;138(1):25-39.
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