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Home / Equine Research Bank / Tissue Eng Part A / MSC frequency correlates with blood vessel density in equine...
Tissue engineering. Part A2008; 15(2); 221-229; doi: 10.1089/ten.tea.2008.0103

MSC frequency correlates with blood vessel density in equine adipose tissue.

Abstract: Mesenchymal stem cells (MSCs) are multipotent cells that have the capacity to develop into different mature mesenchymal cell types. They were originally isolated from bone marrow, but MSC-like cells have also been isolated from other tissues. The common feature of all of these tissues is that they all house blood vessels. It is, thus, possible that MSCs are associated with perivascular locations. The objective of this work was to test the hypothesis that MSCs are associated with blood vessels by verifying if MSC frequency positively correlates with blood vessel density. To this end, samples from highly and poorly vascularized adipose tissue sites of two equine donors were collected and processed for histology and cell isolation. MSC frequency in these samples was estimated by means of CFU-F assays, which were performed under MSC conditions. Culture-adherent cells from equine adipose tissue and bone marrow were culture expanded, tested for differentiation into mesenchymal cell types in vitro, and implanted in vivo in porous ceramic vehicles to assess their osteogenic capacity, using human MSCs and brain pericytes as controls. The differentiation assays showed a difference between adipose tissue-derived cells as compared to equine bone marrow MSCs. While differences in CFU-F frequencies between both donors were evident, the CFU-F numbers correlated directly with blood vessel densities (r(2) = 0.86). We consider these preliminary data as further evidence linking MSCs to blood vessels.
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Publication Date: 2008-10-14 PubMed ID: 18847356PubMed Central: PMC2810211DOI: 10.1089/ten.tea.2008.0103Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • N.I.H.
  • Extramural
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study aims to understand the connection between Mesenchymal stem cells (MSCs) and blood vessels, specifically in equine adipose tissue. Their findings suggest a correlation between the density of blood vessels and the frequency of MSCs.

Objective of the Research

  • The main goal of the study was to verify if the frequency of Mesenchymal stem cells (MSCs) correlates with the density of blood vessels. This attempt was rooted in the hypothesis that MSCs are indeed linked to perivascular locations, as they are initially found in tissues housing blood vessels.

Methodology

  • The researchers collected samples from two equine donors. High and low vascularized adipose tissue sites were selected. These samples were then processed for cell isolation and histology.
  • The frequency of MSCs was calculated by conducting CFU-F assays (Colony-Forming Unit-Fibroblast assays) under conditions suitable for MSCs. Through this method, they could quantify the number of cells capable of dividing and forming a colony.
  • To test the cells’ ability to mature into different types of mesenchymal cells in vitro, culture-adherent cells from equine adipose tissue and bone marrow were expanded in a culture. Subsequently, these cells were implanted in vivo in porous ceramic vehicles to assess their osteogenic, or bone-forming, capacity. Human MSCs and brain pericytes (perivascular cells found in the brain) were used as controls.

Findings and Conclusion

  • The results indicated a difference in the differentiation capacity of cells derived from adipose tissue compared to those derived from equine bone marrow. This evidence points to the versatility of MSCs in terms of their site of origin and their ability to develop into diverse mature cell types.
  • Even though the CFU-F frequencies differed between the two donors, a positive correlation was observed between the CFU-F numbers and blood vessel densities, with a correlation coefficient (r2) of 0.86.
  • These preliminary findings provide evidence that further supports the theory of MSCs’ association with blood vessels. This could have significant implications for understanding the behaviour and potential therapeutic uses of MSCs in regenerative medicine.

Cite This Article

APA
da Silva Meirelles L, Sand TT, Harman RJ, Lennon DP, Caplan AI. (2008). MSC frequency correlates with blood vessel density in equine adipose tissue. Tissue Eng Part A, 15(2), 221-229. https://doi.org/10.1089/ten.tea.2008.0103

Publication

Tissue engineering. Part A
ISSN: 1937-3341
NlmUniqueID: 101466659
Country: United States
Language: English
Volume: 15
Issue: 2
Pages: 221-229

Researcher Affiliations

da Silva Meirelles, Lindolfo
  • Departamento de Genética, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. lindolfo_meirelles@hotmail.com
Sand, Theodore T
    Harman, Robert J
      Lennon, Donald P
        Caplan, Arnold I

          MeSH Terms

          • Adipogenesis
          • Adipose Tissue / cytology
          • Alkaline Phosphatase / metabolism
          • Animals
          • Biological Assay
          • Blood Vessels / cytology
          • Bone Marrow Cells / cytology
          • Calcium / metabolism
          • Cell Count
          • Chondrogenesis
          • Colony-Forming Units Assay
          • Fibroblasts / cytology
          • Horses / physiology
          • Mesenchymal Stem Cells / cytology
          • Mesenchymal Stem Cells / enzymology
          • Osteogenesis

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