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Home / Equine Research Bank / Genes (Basel) / MSTN Regulatory Network in Mongolian Horse Muscle Satellite ...
Genes2022; 13(10); doi: 10.3390/genes13101836

MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies.

Abstract: Myostatin (MSTN), a member of the transforming growth factor-β superfamily, inhibits the activation of muscle satellite cells. However, the role and regulatory network of MSTN in equine muscle cells are not well understood yet. We discovered that MSTN knockdown significantly reduces the proliferation rate of equine muscle satellite cells. In addition, after the RNA sequencing of equine satellite cells transfected with MSTN-interference plasmid and control plasmid, an analysis of the differentially expressed genes was carried out. It was revealed that MSTN regulatory networks mainly involve genes related to muscle function and cell-cycle regulation, and signaling pathways, such as Notch, MAPK, and WNT. Subsequent real-time PCR in equine satellite cells and immunohistochemistry on newborn and adult muscle also verified the MSTN regulatory network found in RNA sequencing analysis. The results of this study provide new insight into the regulatory mechanism of equine MSTN.
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Publication Date: 2022-10-11 PubMed ID: 36292721PubMed Central: PMC9601437DOI: 10.3390/genes13101836Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study focuses on understanding the role of Myostatin (MSTN), a protein that inhibits muscle satellite cell activation, in Mongolian horse muscle cells. Researchers found that reducing MSTN levels slows down the proliferation of these muscle cells, and discovered pathways and genes that MSTN might control, offering new insight into its regulatory mechanisms in horses.

Research Background and Purpose

  • Myostatin (MSTN) is a protein belonging to the transforming growth factor-beta (TGF-β) family, known to inhibit the activation of muscle satellite cells, which are important for muscle development and repair.
  • The role and regulatory function of MSTN in equine muscle cells, particularly in Mongolian horses, is not completely understood.
  • The researchers sought to investigate the effects of MSTN depletion on equine muscle cell proliferation and uncover the underlying regulatory network.

Research Methods and Findings

  • The researchers used RNA interference technologies to silence the MSTN gene in equine muscle satellite cells.
  • They observed a significant reduction in the proliferation rate of these cells following MSTN knockdown (gene silencing), indicating its importance for cell growth and division.
  • RNA sequencing of MSTN-interfered and control satellite cells allowed researchers to identify differentially expressed genes due to MSTN manipulation.
  • The data revealed that MSTN regulatory networks are mostly associated with genes involved in muscle function and cell-cycle regulation, as well as signaling pathways such as the Notch, MAPK, and WNT pathways.
  • These results were further validated with real-time PCR on equine satellite cells and immunohistochemistry studies on newborn and adult muscle tissues.

Significance of the Study

  • This study offers fresh insights into the complex function of MSTN in equine muscle satellite cells by portraying its potential regulatory network.
  • The findings could prove beneficial for understanding and potentially manipulating muscle growth and repair in horses, with possible implications for other species as well.

Cite This Article

APA
Budsuren U, Ulaangerel T, Shen Y, Liu G, Davshilt T, Yi M, Bold D, Zhang X, Bai D, Dorjgotov D, Davaakhuu G, Jambal T, Li B, Du M, Dugarjav M, Bou G. (2022). MSTN Regulatory Network in Mongolian Horse Muscle Satellite Cells Revealed with miRNA Interference Technologies. Genes (Basel), 13(10). https://doi.org/10.3390/genes13101836

Publication

Genes
ISSN: 2073-4425
NlmUniqueID: 101551097
Country: Switzerland
Language: English
Volume: 13
Issue: 10

Researcher Affiliations

Budsuren, Undarmaa
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
  • School of Animal Science and Biotechnology, Mongolian University of Life Sciences, Ulaanbaatar 17024, Mongolia.
Ulaangerel, Tseweendolmaa
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Shen, Yingchao
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Liu, Guiqin
  • College of Agronomy and Agricultural Engineering, Liaocheng University, Liaocheng 252000, China.
Davshilt, Toli
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Yi, Minna
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bold, Demuul
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Zhang, Xinzhuang
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bai, Dongyi
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dorjgotov, Dulguun
  • School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia.
Davaakhuu, Gantulga
  • Institute of Biology, Mongolian Academy of Science, Ulaanbaatar 13330, Mongolia.
Jambal, Tuyatsetseg
  • School of Industrial Technology, Mongolian University of Science and Technology, Ulaanbaatar 14191, Mongolia.
Li, Bei
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Du, Ming
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Dugarjav, Manglai
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.
Bou, Gerelchimeg
  • Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China.

MeSH Terms

  • Horses / genetics
  • Animals
  • Myostatin / genetics
  • Myostatin / metabolism
  • MicroRNAs / genetics
  • Myoblasts / metabolism
  • Muscles / metabolism
  • Transforming Growth Factors

Conflict of Interest Statement

The authors declare no competing interests.

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