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Home / Equine Research Bank / Eur J Appl Physiol Occup Physiol / Muscle ATP loss and lactate accumulation at different work i...
European journal of applied physiology and occupational physiology1991; 62(4); 235-244; doi: 10.1007/BF00571546

Muscle ATP loss and lactate accumulation at different work intensities in the exercising Thoroughbred horse.

Abstract: The effect of 2 min treadmill exercise, at speeds of 6-12 m.s-1 on an incline of 5 degrees, upon muscle adenine nucleotide loss and lactate accumulation was studied in six Thoroughbred horses. Minimal change occurred in the adenosine triphosphate (ATP) content of the middle gluteal muscle at speeds of 10 m.s-1 or less, but significant loss (up to 47%) had occurred in all horses by 12 m.s-1. The decline in ATP significantly correlated with the accumulation of muscle lactate, beginning shortly after the accumulation of 40 mmol.kg-1 dry muscle lactate. Decline in muscle ATP was mirrored closely by the appearance of ammonia, and to a lesser extent, hypoxanthine and uric acid in plasma. The results suggest that peak accumulation of any of these, or simply the concentration at a specified recovery time, may be used as a measure of ATP loss in the musculature as a whole. This was not so in the case of xanthine, which may also be formed from the degradation of guanidine nucleotides. An In-In plot of plasma ammonia against treadmill speed indicated a break point in accumulation between 8 and 9 m.s-1. The kinetics of ammonia accumulation with speed differed from those of lactate.
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Publication Date: 1991-01-01 PubMed ID: 2044532DOI: 10.1007/BF00571546Google Scholar: Lookup
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  • Journal Article
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigated the effect of treadmill exercise on muscle ATP loss and lactate build-up in thoroughbred horses. It found that significant ATP loss occurred at speeds of 12 m/s, correlating with increased muscle lactate and other physiological changes.

Introduction and Methodology

  • The study focused on evaluating the loss of adenosine triphosphate (ATP) and the accumulation of lactate in the muscles of six Thoroughbred horses during two minutes of treadmill exercise.
  • The exercises were conducted at varying intensities, specifically speeds between 6-12 m/s at an inclination of 5°.
  • The middle gluteal muscle was primarily studied for changes in ATP content and lactate accumulation.

Observations and Findings

  • The research showed that up to speeds of 10 m/s, there was minimal change in the ATP content of the horses’ gluteal muscles.
  • However, a significant loss of ATP (up to 47%) was observed when the speed reached 12 m/s in all tested horses. This marked decrease in ATP showed a strong correlation with lactate build-up in the muscle tissue, particularly after reaching an accumulation level of 40 mmol.kg-1 dry muscle lactate.
  • This decline in ATP content was simultaneously accompanied by the increased detection of ammonia, and to a lesser extent, hypoxanthine and uric acid in the horses’ plasma.
  • An interesting finding was that the peak accumulation of these substances or their concentrations at a specified recovery time could be indicators of overall muscle ATP loss.

Analysis and Conclusion

  • The results hinted at a correlation between physical exercise intensity and muscle ATP loss in horses, which also influenced lactate and other metabolic substances’ accumulation.
  • However, the accumulation kinetics of lactate and ammonia showed differences, indicating distinct physiological mechanisms underlying their production and clearance during strenuous exercise.
  • There seemed to be a tipping point between 8 and 9 m/s, where the accumulation of plasma ammonia drastically increased. This showed a nonlinear relationship with increasing treadmill speed.
  • One surprising observation was that xanthine, another metabolic substrate known to be formed from the degradation of guanidine nucleotides, was not a reliable measure of muscle ATP loss. The researchers pointed out that this anomaly needs further exploration and understanding.

Cite This Article

APA
Harris RC, Marlin DJ, Snow DH, Harkness RA. (1991). Muscle ATP loss and lactate accumulation at different work intensities in the exercising Thoroughbred horse. Eur J Appl Physiol Occup Physiol, 62(4), 235-244. https://doi.org/10.1007/BF00571546

Publication

European journal of applied physiology and occupational physiology
ISSN: 0301-5548
NlmUniqueID: 0410266
Country: Germany
Language: English
Volume: 62
Issue: 4
Pages: 235-244

Researcher Affiliations

Harris, R C
  • Department of Comparative Physiology, Animal Health Trust, Newmarket, England.
Marlin, D J
    Snow, D H
      Harkness, R A

        MeSH Terms

        • Adenosine Triphosphate / metabolism
        • Animals
        • Female
        • Horses / metabolism
        • Horses / physiology
        • Lactates / metabolism
        • Male
        • Muscles / metabolism
        • Physical Exertion

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        Citations

        This article has been cited 11 times.
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