Mycobacterium bovis infection in a horse with granulomatous enterocolitis.
Abstract: A 2-year-old dappled Percheron horse had a wasting condition that did not respond to antibiotic treatments and ultimately resulted in death. Thickening of the wall of the large colon and enlargement of the mesenteric lymph nodes were observed at postmortem examination, along with the presence of pinpoint whitish foci in the liver. Microscopic examination of affected tissues revealed diffuse chronic granulomatous enterocolitis, granulomatous mesenteric lymphadenitis, and multifocal granulomatous hepatitis. The DNA extracted from paraffin-embedded intestinal and lymph node samples was analyzed using both a polymerase chain reaction (PCR) assay and PCR-restriction endonuclease analysis and demonstrated the presence of Mycobacterium bovis.
© 2015 The Author(s).
Publication Date: 2015-02-12 PubMed ID: 25677270DOI: 10.1177/1040638715571359Google Scholar: Lookup
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Summary
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This study presents the case of a young horse which died due to a bacterial infection known as Mycobacterium bovis. The disease manifested as a wasting condition and caused inflammation and formation of granulomas in various tissues, revealed during microscopic examination of tissue samples.
Study Subject and Medical History
- The research revolves around a 2-year-old dappled Percheron horse, which was displaying a wasting condition characterized by progressive weight loss and general decline in health.
- Despite treatment with antibiotics, the horse’s condition did not improve and eventually led to its death.
Postmortem Examination
- Upon postmortem examination, the researchers observed thickening of the wall of the large colon and enlargement of the mesenteric lymph nodes—the glands responsible for the production and storage of cells that fight infection and disease.
- Additionally, they noted the presence of pinpoint whitish foci, indicative of disease or inflammation, in the liver.
- Microscopic examination of the affected tissues revealed diffuse chronic granulomatous enterocolitis, granulomatous mesenteric lymphadenitis, and multifocal granulomatous hepatitis. These conditions involve inflammation and the formation of granulomas, which are clusters of immune cells, in response to persistent non-degradable pathogens, foreign substances or irritants.
Disease Identification
- To identify the disease-causing agent, the researchers analyzed the DNA extracted from paraffin-embedded intestinal and lymph node samples.
- They made use of a polymerase chain reaction (PCR) assay and PCR-restriction endonuclease analysis—molecular biology techniques that generate millions of copies of a DNA sequence and allow the identification of the DNA, respectively.
- The analyses confirmed the presence of Mycobacterium bovis, a bacteria that primarily infects cattle but can also cause tuberculosis in humans and other animals.
Cite This Article
APA
Sarradell JE, Alvarez J, Biscia M, Zumarraga M, Wunschmann A, Armien AG, Perez AM.
(2015).
Mycobacterium bovis infection in a horse with granulomatous enterocolitis.
J Vet Diagn Invest, 27(2), 203-205.
https://doi.org/10.1177/1040638715571359 Publication
Researcher Affiliations
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga) jsarrade@fveter.unr.edu.ar.
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
- Department of General and Systemic Pathology, Veterinary Faculty, National University of Rosario, Casilda, Argentina (Sarradell, Biscia)Department of Veterinary Population Medicine (Alvarez, Perez), College of Veterinary Medicine, University of Minnesota, St. Paul, MNVeterinary Diagnostic Laboratory (Wunschmann, Armien), College of Veterinary Medicine, University of Minnesota, St. Paul, MNBiotechnology Institute, Centre of Agronomy and Veterinary Sciences, National Agricultural Technology Institute (INTA), Hurlingham, Buenos Aires, Argentina (Zumarraga).
MeSH Terms
- Animals
- DNA, Bacterial / analysis
- Enterocolitis / complications
- Enterocolitis / veterinary
- Fatal Outcome
- Horse Diseases / diagnosis
- Horse Diseases / microbiology
- Horse Diseases / pathology
- Horses
- Lymph Nodes / pathology
- Lymphadenitis / complications
- Lymphadenitis / veterinary
- Mycobacterium Infections / complications
- Mycobacterium Infections / diagnosis
- Mycobacterium Infections / veterinary
- Mycobacterium bovis / genetics
- Mycobacterium bovis / isolation & purification
- Polymerase Chain Reaction / veterinary
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